Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites

Detalhes bibliográficos
Autor(a) principal: Mosquera, Katherine D.
Data de Publicação: 2023
Outros Autores: Villegas, Luis Eduardo Martínez, Fernandes, Gabriel Rocha, David, Mariana Rocha, Freitas, Rafael Maciel de, Moreira, Luciano A., Lorenzo, Marcelo G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/58192
Resumo: Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento Vetorial e Interação com Patógenos. Belo Horizonte, MG, Brasil.
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spelling Mosquera, Katherine D.Villegas, Luis Eduardo MartínezFernandes, Gabriel RochaDavid, Mariana RochaFreitas, Rafael Maciel deMoreira, Luciano A.Lorenzo, Marcelo G.2023-05-06T20:41:08Z2023-05-06T20:41:08Z2023MOSQUERA, Katherine D. et al. Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites. BMC Biology, v. 21, 97, p. 1 - 15, 2023.1741-7007https://www.arca.fiocruz.br/handle/icict/58192engBMCAedes aegyptiMicrobiotaLocais de reproduçãoConstrução de nichoOviposiçãoAedes aegyptiBreeding sitesOvipositionNiche constructionMicrobiotaEgg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sitesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento Vetorial e Interação com Patógenos. Belo Horizonte, MG, Brasil.Department of Entomology, The Ohio State University, 2001 Fyffe Rd., Room 232 Howlett Hall, Columbus, OH 43210, USA / Fundação Oswaldo Cruz. Instituto René Rachou. Vetores de Mosquito: Grupo de Interações de Endossimbiontes e Patógeno-Vetor. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou / Biossistemas Informática. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de hematiozoários. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de hematiozoários. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Vetores de Mosquito: Grupo de Interações de Endossimbiontes e Patógeno-Vetor. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento Vetorial e Interação com Patógenos. Belo Horizonte, MG, Brasil.Background Aedes aegypti, the main arboviral mosquito vector, is attracted to human dwellings and makes use of human-generated breeding sites. Past research has shown that bacterial communities associated with such sites undergo compositional shifts as larvae develop and that exposure to different bacteria during larval stages can have an impact on mosquito development and life-history traits. Based on these facts, we hypothesized that female Ae. aegypti shape the bacteria communities of breeding sites during oviposition as a form of niche construction to favor offspring fitness. Results To test this hypothesis, we first verified that gravid females can act as mechanical vectors of bacteria. We then elaborated an experimental scheme to test the impact of oviposition on breeding site microbiota. Five different groups of experimental breeding sites were set up with a sterile aqueous solution of larval food, and subsequently exposed to (1) the environment alone, (2) surface-sterilized eggs, (3) unsterilized eggs, (4) a non-egg laying female, or (5) oviposition by a gravid female. The microbiota of these differently treated sites was assessed by amplicon-oriented DNA sequencing once the larvae from the sites with eggs had completed development and formed pupae. Microbial ecology analyses revealed significant differences between the five treatments in terms of diversity. In particular, between-treatment shifts in abundance profiles were detected, showing that females induce a significant decrease in microbial alpha diversity through oviposition. In addition, indicator species analysis pinpointed bacterial taxa with significant predicting values and fidelity coefficients for the samples in which single females laid eggs. Furthermore, we provide evidence regarding how one of these indicator taxa, Elizabethkingia, exerts a positive effect on the development and fitness of mosquito larvae. Conclusions Ovipositing females impact the composition of the microbial community associated with a breeding site, promoting certain bacterial taxa over those prevailing in the environment. Among these bacteria, we found known mosquito symbionts and showed that they can improve offspring fitness if present in the water where eggs are laid. We deem this oviposition-mediated bacterial community shaping as a form of niche construction initiated by the gravid female.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/58192/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRapahelFreitas_MarianaDavid_etal-IOC_2023.pdfRapahelFreitas_MarianaDavid_etal-IOC_2023.pdfapplication/pdf2919964https://www.arca.fiocruz.br/bitstream/icict/58192/2/RapahelFreitas_MarianaDavid_etal-IOC_2023.pdffef20867c95a55bf988b8bedfbd3d793MD52icict/581922023-09-04 10:37:09.607oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-04T13:37:09Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
title Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
spellingShingle Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
Mosquera, Katherine D.
Aedes aegypti
Microbiota
Locais de reprodução
Construção de nicho
Oviposição
Aedes aegypti
Breeding sites
Oviposition
Niche construction
Microbiota
title_short Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
title_full Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
title_fullStr Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
title_full_unstemmed Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
title_sort Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites
author Mosquera, Katherine D.
author_facet Mosquera, Katherine D.
Villegas, Luis Eduardo Martínez
Fernandes, Gabriel Rocha
David, Mariana Rocha
Freitas, Rafael Maciel de
Moreira, Luciano A.
Lorenzo, Marcelo G.
author_role author
author2 Villegas, Luis Eduardo Martínez
Fernandes, Gabriel Rocha
David, Mariana Rocha
Freitas, Rafael Maciel de
Moreira, Luciano A.
Lorenzo, Marcelo G.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mosquera, Katherine D.
Villegas, Luis Eduardo Martínez
Fernandes, Gabriel Rocha
David, Mariana Rocha
Freitas, Rafael Maciel de
Moreira, Luciano A.
Lorenzo, Marcelo G.
dc.subject.other.en_US.fl_str_mv Aedes aegypti
Microbiota
Locais de reprodução
Construção de nicho
Oviposição
topic Aedes aegypti
Microbiota
Locais de reprodução
Construção de nicho
Oviposição
Aedes aegypti
Breeding sites
Oviposition
Niche construction
Microbiota
dc.subject.en.en_US.fl_str_mv Aedes aegypti
Breeding sites
Oviposition
Niche construction
Microbiota
description Fundação Oswaldo Cruz. Instituto René Rachou. Grupo de Comportamento Vetorial e Interação com Patógenos. Belo Horizonte, MG, Brasil.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-05-06T20:41:08Z
dc.date.available.fl_str_mv 2023-05-06T20:41:08Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MOSQUERA, Katherine D. et al. Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites. BMC Biology, v. 21, 97, p. 1 - 15, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/58192
dc.identifier.issn.en_US.fl_str_mv 1741-7007
identifier_str_mv MOSQUERA, Katherine D. et al. Egg‑laying by female Aedes aegypti shapes the bacterial communities of breeding sites. BMC Biology, v. 21, 97, p. 1 - 15, 2023.
1741-7007
url https://www.arca.fiocruz.br/handle/icict/58192
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/58192/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/58192/2/RapahelFreitas_MarianaDavid_etal-IOC_2023.pdf
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