Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27510 |
Resumo: | Programa Ciências sem fronteiras Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) of Brazil and Brasil SemMiséria Program of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superios (Capes) of Brazil. |
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Santos, Luciane AmorimAdhikarla, HarithaYan, XitingWang, ZhengFouts, Derrick EVinetz, Joseph MAlcantara, Luiz Carlos JúniorHartskeerl, Rudy AGoris, Marga G. APicardeau, MathieuReis, Mitermayer Galvão dosTownsend, Jeffrey PZhao, HongyuKo, Albert IcksangWunder Junior, Elsio Augusto2018-07-12T16:27:00Z2018-07-12T16:27:00Z2018SANTOS, L. A. et al. Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel. Frontiers in Cellular and Infection Microbiology, v. 8, art. 193, 2018.2235-2988https://www.arca.fiocruz.br/handle/icict/27510Programa Ciências sem fronteiras Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) of Brazil and Brasil SemMiséria Program of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superios (Capes) of Brazil.Yale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United StatesYale School of Public Health. Department of Biostatistics. New Haven, CT, United StatesYale School of Public Health. Department of Biostatistics. New Haven, CT, United StatesJ. Craig Venter Institute. Rockville, MD, United StatesFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilRoyal Tropical Institute. KIT Biomedical Research. Amsterdam, NetherlandsRoyal Tropical Institute. KIT Biomedical Research. Amsterdam, NetherlandsInstitut Pasteur. ,Biology of Spirochetes Unit. Paris, FranceFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilYale School of Public Health. Department of Biostatistics. New Haven, CT, United StatesYale School of Public Health. Department of Biostatistics. New Haven, CT, United StatesYale School of Public Health. Department of Epidemiology of Microbial Diseases. New Haven, CT, United States / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilLeptospirosis is a worldwide zoonosis, responsible for more than 1 million cases and 60,000 deaths every year. Among the 13 pathogenic species of the genus Leptospira, serovars belonging to L. interrogans serogroup Icterohaemorrhagiae are considered to be the most virulent strains, and responsible for majority of the reported severe cases. Serovars Copenhageni and Icterohaemorrhagiae are major representatives of this serogroup and despite their public health relevance, little is known regarding the genetic differences between these two serovars. In this study, we analyzed the genome sequences of 67 isolates belonging to L. interrogans serovars Copenhageni and Icterohaemorrhagiae to investigate the influence of spatial and temporal variations on DNA sequence diversity. Out of the 1072 SNPs identified, 276 were in non-coding regions and 796 in coding regions. Indel analyses identified 258 indels, out of which 191 were found in coding regions and 67 in non-coding regions. Our phylogenetic analyses based on SNP dataset revealed that both serovars are closely related but showed distinct spatial clustering. However, likelihood ratio test of the indel data statistically confirmed the presence of a frameshift mutation within a homopolymeric tract of lic12008 gene (related to LPS biosynthesis) in all the L. interrogans serovar Icterohaemorrhagiae strains but not in the Copenhageni strains. Therefore, this internal indel identified can genetically distinguish L. interrogans serovar Copenhageni from serovar Icterohaemorrhagiae with high discriminatory power. To our knowledge, this is the first study to identify global sequence variations (SNPs and Indels) in L. interrogans serovars Copenhageni and Icterohaemorrhagiae.engFrontiers MediaLeptospiraLeptospiroseCopenhageniIcterohaemorrhagiaeSequenciamento do genoma completoSNPsIndelsFilogeniaLeptospiraLeptospirosisCopenhageniIcterohaemorrhagiaeWhole-genome sequencingSNPsIndelsPhylogenyGenomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27510/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSantos LA Genomic Comparison Among Global...pdfSantos LA Genomic Comparison Among 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| dc.title.pt_BR.fl_str_mv |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| title |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| spellingShingle |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel Santos, Luciane Amorim Leptospira Leptospirose Copenhageni Icterohaemorrhagiae Sequenciamento do genoma completo SNPs Indels Filogenia Leptospira Leptospirosis Copenhageni Icterohaemorrhagiae Whole-genome sequencing SNPs Indels Phylogeny |
| title_short |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| title_full |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| title_fullStr |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| title_full_unstemmed |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| title_sort |
Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel |
| author |
Santos, Luciane Amorim |
| author_facet |
Santos, Luciane Amorim Adhikarla, Haritha Yan, Xiting Wang, Zheng Fouts, Derrick E Vinetz, Joseph M Alcantara, Luiz Carlos Júnior Hartskeerl, Rudy A Goris, Marga G. A Picardeau, Mathieu Reis, Mitermayer Galvão dos Townsend, Jeffrey P Zhao, Hongyu Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| author_role |
author |
| author2 |
Adhikarla, Haritha Yan, Xiting Wang, Zheng Fouts, Derrick E Vinetz, Joseph M Alcantara, Luiz Carlos Júnior Hartskeerl, Rudy A Goris, Marga G. A Picardeau, Mathieu Reis, Mitermayer Galvão dos Townsend, Jeffrey P Zhao, Hongyu Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Luciane Amorim Adhikarla, Haritha Yan, Xiting Wang, Zheng Fouts, Derrick E Vinetz, Joseph M Alcantara, Luiz Carlos Júnior Hartskeerl, Rudy A Goris, Marga G. A Picardeau, Mathieu Reis, Mitermayer Galvão dos Townsend, Jeffrey P Zhao, Hongyu Ko, Albert Icksang Wunder Junior, Elsio Augusto |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospira Leptospirose Copenhageni Icterohaemorrhagiae Sequenciamento do genoma completo SNPs Indels Filogenia |
| topic |
Leptospira Leptospirose Copenhageni Icterohaemorrhagiae Sequenciamento do genoma completo SNPs Indels Filogenia Leptospira Leptospirosis Copenhageni Icterohaemorrhagiae Whole-genome sequencing SNPs Indels Phylogeny |
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Leptospira Leptospirosis Copenhageni Icterohaemorrhagiae Whole-genome sequencing SNPs Indels Phylogeny |
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Programa Ciências sem fronteiras Conselho Nacional de Desenvolvimento Cientifico e Tecnológico (CNPq) of Brazil and Brasil SemMiséria Program of the Coordenação de Aperfeiçoamento de Pessoal de Nível Superios (Capes) of Brazil. |
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2018 |
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2018-07-12T16:27:00Z |
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2018-07-12T16:27:00Z |
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2018 |
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SANTOS, L. A. et al. Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel. Frontiers in Cellular and Infection Microbiology, v. 8, art. 193, 2018. |
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https://www.arca.fiocruz.br/handle/icict/27510 |
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2235-2988 |
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SANTOS, L. A. et al. Genomic Comparison Among Global Isolates of L. interrogans Serovars Copenhageni and Icterohaemorrhagiae Identified Natural Genetic Variation Caused by an Indel. Frontiers in Cellular and Infection Microbiology, v. 8, art. 193, 2018. 2235-2988 |
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