Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection

Detalhes bibliográficos
Autor(a) principal: Torices, Silvia
Data de Publicação: 2021
Outros Autores: Cabrera, Rosalba, Stangis, Michael, Naranjo, Oaindy, Fattakhov, Nikolai, Teglas, Timea, Adesse, Daniel, Toborek, Michal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/48452
Resumo: Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.
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spelling Torices, SilviaCabrera, RosalbaStangis, MichaelNaranjo, OaindyFattakhov, NikolaiTeglas, TimeaAdesse, DanielToborek, Michal2021-07-31T13:55:22Z2021-07-31T13:55:22Z2021TORICES, Silvia et al. Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection. Journal of Neuroinflamation, v.18, n. 167, 16 p, 2021.1742-2094https://www.arca.fiocruz.br/handle/icict/4845210.1186/s12974-021-02210-2engBMCSARS-CoV-2HIV-1Barreira hemencefálicaEnzima de Conversão de Angiotensina 2SARS-CoV-2Blood-brain barrierHIV-1ACE2TMPRSS2Enzima de Conversão de Angiotensina 2Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDepartment of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural Rio de Janeiro, RJ, Brasil.Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.Background: Neurological complications are common in patients affected by COVID-19 due to the ability of SARS CoV-2 to infect brains. While the mechanisms of this process are not fully understood, it has been proposed that SARS-CoV-2 can infect the cells of the neurovascular unit (NVU), which form the blood-brain barrier (BBB). The aim of the current study was to analyze the expression pattern of the main SARS-CoV-2 receptors in naïve and HIV-1- infected cells of the NVU in order to elucidate a possible pathway of the virus entry into the brain and a potential modulatory impact of HIV-1 in this process. Methods: The gene and protein expression profile of ACE2, TMPRSS2, ADAM17, BSG, DPP4, AGTR2, ANPEP, cathepsin B, and cathepsin L was assessed by qPCR, immunoblotting, and immunostaining, respectively. In addition, we investigated if brain endothelial cells can be affected by the exposure to the S1 subunit of the S protein, the domain responsible for the direct binding of SARS-CoV-2 to the ACE2 receptors. Results: The receptors involved in SARS-CoV-2 infection are co-expressed in the cells of the NVU, especially in astrocytes and microglial cells. These receptors are functionally active as exposure of endothelial cells to the SARS CoV-2 S1 protein subunit altered the expression pattern of tight junction proteins, such as claudin-5 and ZO-1. Additionally, HIV-1 infection upregulated ACE2 and TMPRSS2 expression in brain astrocytes and microglia cells. Conclusions: These findings provide key insight into SARS-CoV-2 recognition by cells of the NVU and may help to develop possible treatment of CNS complications of COVID-19.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48452/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDanielAdesse_etal_IOC_2021.pdfDanielAdesse_etal_IOC_2021.pdfapplication/pdf3124488https://www.arca.fiocruz.br/bitstream/icict/48452/2/DanielAdesse_etal_IOC_2021.pdf99cf2df33b1de63d3081e6cf13d977d4MD52TEXTDanielAdesse_etal_IOC_2021.pdf.txtDanielAdesse_etal_IOC_2021.pdf.txtExtracted texttext/plain69008https://www.arca.fiocruz.br/bitstream/icict/48452/3/DanielAdesse_etal_IOC_2021.pdf.txta65bd213e0939a562eb5edc375d8e2e3MD53icict/484522021-08-01 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dc.title.pt_BR.fl_str_mv Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
title Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
spellingShingle Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
Torices, Silvia
SARS-CoV-2
HIV-1
Barreira hemencefálica
Enzima de Conversão de Angiotensina 2
SARS-CoV-2
Blood-brain barrier
HIV-1
ACE2
TMPRSS2
Enzima de Conversão de Angiotensina 2
title_short Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
title_full Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
title_fullStr Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
title_full_unstemmed Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
title_sort Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection
author Torices, Silvia
author_facet Torices, Silvia
Cabrera, Rosalba
Stangis, Michael
Naranjo, Oaindy
Fattakhov, Nikolai
Teglas, Timea
Adesse, Daniel
Toborek, Michal
author_role author
author2 Cabrera, Rosalba
Stangis, Michael
Naranjo, Oaindy
Fattakhov, Nikolai
Teglas, Timea
Adesse, Daniel
Toborek, Michal
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Torices, Silvia
Cabrera, Rosalba
Stangis, Michael
Naranjo, Oaindy
Fattakhov, Nikolai
Teglas, Timea
Adesse, Daniel
Toborek, Michal
dc.subject.other.pt_BR.fl_str_mv SARS-CoV-2
HIV-1
Barreira hemencefálica
Enzima de Conversão de Angiotensina 2
topic SARS-CoV-2
HIV-1
Barreira hemencefálica
Enzima de Conversão de Angiotensina 2
SARS-CoV-2
Blood-brain barrier
HIV-1
ACE2
TMPRSS2
Enzima de Conversão de Angiotensina 2
dc.subject.en.pt_BR.fl_str_mv SARS-CoV-2
Blood-brain barrier
HIV-1
ACE2
TMPRSS2
dc.subject.decs.pt_BR.fl_str_mv Enzima de Conversão de Angiotensina 2
description Department of Biochemistry and Molecular Biology, University of Miami Miller School of Medicine. Miami, FL, USA.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-07-31T13:55:22Z
dc.date.available.fl_str_mv 2021-07-31T13:55:22Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TORICES, Silvia et al. Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection. Journal of Neuroinflamation, v.18, n. 167, 16 p, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/48452
dc.identifier.issn.pt_BR.fl_str_mv 1742-2094
dc.identifier.doi.none.fl_str_mv 10.1186/s12974-021-02210-2
identifier_str_mv TORICES, Silvia et al. Expression of SARS-CoV-2-related receptors in cells of the neurovascular unit: implications for HIV-1 infection. Journal of Neuroinflamation, v.18, n. 167, 16 p, 2021.
1742-2094
10.1186/s12974-021-02210-2
url https://www.arca.fiocruz.br/handle/icict/48452
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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