American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/37704 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Programa de Pós-graduação em Inovação Terapêutica. Recife, PE, Brasil. |
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Mendes, Andresa Pereira OliveiraOliveira, Beatriz CoutinhoPereira, Allana Maria S.Castro, Maria Carolina Accioly BrelazSouza, Marina AssisBrito, Maria Edileuza FelintoAraújo, Fernanda FortesTeixeira-Carvalho, AndreaMartins-Filho, Olindo AssisPereira, Valeria Rêgo Alves2019-12-09T13:18:25Z2019-12-09T13:18:25Z2019MENDES, Andresa Pereira Oliveira et al. American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification. BMC Infectious Diseases, v. 19, n. 1, p. 1-11, Nov. 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/3770410.1186/s12879-019-4642-7engAmerican tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identificationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Programa de Pós-graduação em Inovação Terapêutica. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Programa de Pós-graduação em Inovação Terapêutica. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Centro Acadêmico de Vitória. Laboratório de Parasitologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Background: The present study aimed to demonstrate the applicability of a flow cytometry-based serology approach to identify spontaneous cure by the detection of immunoglobulin G, and also, the diagnosis and cure criterion by the IgG1 isotype in American Tegumentary Leishmaniasis – ATL caused by L. (V.) braziliensis. Also, a comparison between flow cytometry with the serological conventional technique was performed. Methods: Forty five individuals were included in study. They were assessed in two moments: First, 8 subjects spontaneously cured of ATL, 8 healthy individuals and 15 patients who had a positive diagnosis for ATL were selected before treatment to identify spontaneous cure by immunoglobulin G detection. Secondly, 14 patients who were positive for ATL were selected and had their blood collected before and 1, 2 and 5 years after treatment, respectively, for the diagnostic tests (ELISA and flow cytometry) and cure criterion evaluation using the IgG1 isotype Results: The analysis of the mean percentage of positive fluorescent parasites (PPFP) along with the titration curves of IgG anti-fixed promastigotes of L.(V.)braziliensis, confirmed the applicability of this method for monitoring spontaneous cure in ATL with outstanding co-positivity (100%) and co-negativity (100%) performance indexes. Regarding the results of the comparison between flow cytometry and ELISA it was seen that there was a better accuracy of the first one in relation to the other. When IgG1 applicability was evaluated, it was observed that before treatment, 36.8% of the patients were negative; in patients 1 year post-treatment, 82.3%; 2 years post-treatment, 27.2% and in patients 5 years post-treatment, 87.5%. The overall analysis of the results suggests that flow cytometry can be applied to ATL detection, and that the use of IgG1 isotype has possibilities to contribute as a more specific diagnostic method. Conclusions: Therefore, this area has great perspectives use for the diagnosis and cure criterion, and also it can be scaled up with the possibility to characterize the different clinical stages of the disease. Together, these findings demonstrate the applicability of a flow cytometry-based serology approach and opens up new avenues of research with this technique, such as the understanding the humoral response in ATL patients.ATLCure criterion and spontaneous cureELISAFlow cytometryL. (V.) braziliensisLeishmaniose CutâneaEnsaio de Imunoadsorção EnzimáticaCitometria de Fluxoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83085https://www.arca.fiocruz.br/bitstream/icict/37704/1/license.txt4194663c61da82c21746deb8d6361188MD51ORIGINALAmerican tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes_ a comparative performance of serological tests and spontaneous cure identification.pdfAmerican tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes_ a comparative performance of serological tests and spontaneous cure identification.pdfapplication/pdf1152465https://www.arca.fiocruz.br/bitstream/icict/37704/2/American%20tegumentary%20leishmaniasis%20diagnosis%20using%20L.%20%28V.%29%20braziliensis%20fixed%20promastigotes_%20a%20comparative%20performance%20of%20serological%20tests%20and%20spontaneous%20cure%20identification.pdf9b7ccb5eee8bd72a080cbee44a3879e6MD52TEXTAmerican tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes_ a comparative performance of serological tests and spontaneous cure identification.pdf.txtAmerican tegumentary leishmaniasis diagnosis using L. 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| dc.title.pt_BR.fl_str_mv |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| title |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| spellingShingle |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification Mendes, Andresa Pereira Oliveira ATL Cure criterion and spontaneous cure ELISA Flow cytometry L. (V.) braziliensis Leishmaniose Cutânea Ensaio de Imunoadsorção Enzimática Citometria de Fluxo |
| title_short |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| title_full |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| title_fullStr |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| title_full_unstemmed |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| title_sort |
American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification |
| author |
Mendes, Andresa Pereira Oliveira |
| author_facet |
Mendes, Andresa Pereira Oliveira Oliveira, Beatriz Coutinho Pereira, Allana Maria S. Castro, Maria Carolina Accioly Brelaz Souza, Marina Assis Brito, Maria Edileuza Felinto Araújo, Fernanda Fortes Teixeira-Carvalho, Andrea Martins-Filho, Olindo Assis Pereira, Valeria Rêgo Alves |
| author_role |
author |
| author2 |
Oliveira, Beatriz Coutinho Pereira, Allana Maria S. Castro, Maria Carolina Accioly Brelaz Souza, Marina Assis Brito, Maria Edileuza Felinto Araújo, Fernanda Fortes Teixeira-Carvalho, Andrea Martins-Filho, Olindo Assis Pereira, Valeria Rêgo Alves |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendes, Andresa Pereira Oliveira Oliveira, Beatriz Coutinho Pereira, Allana Maria S. Castro, Maria Carolina Accioly Brelaz Souza, Marina Assis Brito, Maria Edileuza Felinto Araújo, Fernanda Fortes Teixeira-Carvalho, Andrea Martins-Filho, Olindo Assis Pereira, Valeria Rêgo Alves |
| dc.subject.en.pt_BR.fl_str_mv |
ATL Cure criterion and spontaneous cure ELISA Flow cytometry L. (V.) braziliensis |
| topic |
ATL Cure criterion and spontaneous cure ELISA Flow cytometry L. (V.) braziliensis Leishmaniose Cutânea Ensaio de Imunoadsorção Enzimática Citometria de Fluxo |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmaniose Cutânea Ensaio de Imunoadsorção Enzimática Citometria de Fluxo |
| description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil / Universidade Federal de Pernambuco. Programa de Pós-graduação em Inovação Terapêutica. Recife, PE, Brasil. |
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2019 |
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2019-12-09T13:18:25Z |
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2019-12-09T13:18:25Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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MENDES, Andresa Pereira Oliveira et al. American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification. BMC Infectious Diseases, v. 19, n. 1, p. 1-11, Nov. 2019. |
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https://www.arca.fiocruz.br/handle/icict/37704 |
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1471-2334 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s12879-019-4642-7 |
| identifier_str_mv |
MENDES, Andresa Pereira Oliveira et al. American tegumentary leishmaniasis diagnosis using L. (V.) braziliensis fixed promastigotes: a comparative performance of serological tests and spontaneous cure identification. BMC Infectious Diseases, v. 19, n. 1, p. 1-11, Nov. 2019. 1471-2334 10.1186/s12879-019-4642-7 |
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