Binding of Sulpiride to Seric Albumins

Detalhes bibliográficos
Autor(a) principal: Fragoso, Viviane Muniz da Silva
Data de Publicação: 2016
Outros Autores: Coura, Carla Patrícia de Morais, Hoppe, Luanda Yanaan, Soares, Marília Amável Gomes, Silva, Dilson, Cortez, Celia Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/17838
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.
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spelling Fragoso, Viviane Muniz da SilvaCoura, Carla Patrícia de MoraisHoppe, Luanda YanaanSoares, Marília Amável GomesSilva, DilsonCortez, Celia Martins2017-02-16T14:50:57Z2017-02-16T14:50:57Z2016FRAGOSO, Viviane Muniz da Silva; et al. Binding of Sulpiride to Seric Albumins. Int. J. Mol. Sci., v.17, n.1, 10p, 2016.1661-6596https://www.arca.fiocruz.br/handle/icict/1783810.3390/ijms170100591422-0067engMDPIAlbuminasExtinção de fluorescênciaInteraçãoSulpiridaalbuminsfluorescence quenchinginteractionsulpirideBinding of Sulpiride to Seric Albuminsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Pós-Graduação em Ciências Médicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Universidade do Estado do Rio de Janeiro. Matemática Aplicada. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Pós-Graduação em Ciências Médicas. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Matemática Aplicada. Ri de Janeiro, RJ, Brasil. .Universidade do Estado do Rio de Janeiro. Pós-Graduação em Ciências Médicas. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Matemática Aplicada. Rio de Janeiro, RJ, Brasil. .The aim of this work was to study the interaction of sulpiride with human serum albumin (HSA) and bovine serum albumin (BSA) through the fluorescence quenching technique. As sulpiride molecules emit fluorescence, we have developed a simple mathematical model to discriminate the quencher fluorescence from the albumin fluorescence in the solution where they interact. Sulpiride is an antipsychotic used in the treatment of several psychiatric disorders. We selectively excited the fluorescence of tryptophan residues with 290 nm wavelength and observed the quenching by titrating HSA and BSA solutions with sulpiride. Stern-Volmer graphs were plotted and quenching constants were estimated. Results showed that sulpiride form complexes with both albumins. Estimated association constants for the interaction sulpiride-HSA were 2.20 (±0.08) × 10⁴ M(-1), at 37 °C, and 5.46 (±0.20) × 10⁴ M(-1), at 25 °C. Those for the interaction sulpiride-BSA are 0.44 (±0.01) × 10⁴ M(-1), at 37 °C and 2.17 (±0.04) × 10⁴ M(-1), at 25 °C. The quenching intensity of BSA, which contains two tryptophan residues in the peptide chain, was found to be higher than that of HSA, what suggests that the primary binding site for sulpiride in albumin should be located next to the sub domain IB of the protein structure.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/17838/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALluanda2_hoppe_etal_IOC_2016.pdfluanda2_hoppe_etal_IOC_2016.pdfapplication/pdf1101955https://www.arca.fiocruz.br/bitstream/icict/17838/2/luanda2_hoppe_etal_IOC_2016.pdfa3f2a73090dcffb851c978c423bbcfdeMD52TEXTluanda2_hoppe_etal_IOC_2016.pdf.txtluanda2_hoppe_etal_IOC_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Binding of Sulpiride to Seric Albumins
title Binding of Sulpiride to Seric Albumins
spellingShingle Binding of Sulpiride to Seric Albumins
Fragoso, Viviane Muniz da Silva
Albuminas
Extinção de fluorescência
Interação
Sulpirida
albumins
fluorescence quenching
interaction
sulpiride
title_short Binding of Sulpiride to Seric Albumins
title_full Binding of Sulpiride to Seric Albumins
title_fullStr Binding of Sulpiride to Seric Albumins
title_full_unstemmed Binding of Sulpiride to Seric Albumins
title_sort Binding of Sulpiride to Seric Albumins
author Fragoso, Viviane Muniz da Silva
author_facet Fragoso, Viviane Muniz da Silva
Coura, Carla Patrícia de Morais
Hoppe, Luanda Yanaan
Soares, Marília Amável Gomes
Silva, Dilson
Cortez, Celia Martins
author_role author
author2 Coura, Carla Patrícia de Morais
Hoppe, Luanda Yanaan
Soares, Marília Amável Gomes
Silva, Dilson
Cortez, Celia Martins
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fragoso, Viviane Muniz da Silva
Coura, Carla Patrícia de Morais
Hoppe, Luanda Yanaan
Soares, Marília Amável Gomes
Silva, Dilson
Cortez, Celia Martins
dc.subject.other.pt_BR.fl_str_mv Albuminas
Extinção de fluorescência
Interação
Sulpirida
topic Albuminas
Extinção de fluorescência
Interação
Sulpirida
albumins
fluorescence quenching
interaction
sulpiride
dc.subject.en.pt_BR.fl_str_mv albumins
fluorescence quenching
interaction
sulpiride
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-02-16T14:50:57Z
dc.date.available.fl_str_mv 2017-02-16T14:50:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FRAGOSO, Viviane Muniz da Silva; et al. Binding of Sulpiride to Seric Albumins. Int. J. Mol. Sci., v.17, n.1, 10p, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/17838
dc.identifier.issn.pt_BR.fl_str_mv 1661-6596
dc.identifier.doi.none.fl_str_mv 10.3390/ijms17010059
dc.identifier.eissn.none.fl_str_mv 1422-0067
identifier_str_mv FRAGOSO, Viviane Muniz da Silva; et al. Binding of Sulpiride to Seric Albumins. Int. J. Mol. Sci., v.17, n.1, 10p, 2016.
1661-6596
10.3390/ijms17010059
1422-0067
url https://www.arca.fiocruz.br/handle/icict/17838
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/17838/2/luanda2_hoppe_etal_IOC_2016.pdf
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