Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae

Detalhes bibliográficos
Autor(a) principal: Martins, Gustavo Ferreira
Data de Publicação: 2011
Outros Autores: Guedes, Bruno Augusto Maciel, Silva, Luciana Maria, Serrão, José Eduardo, Dias, Consuelo Latorre Fortes, Ortigão, José Marcelo Ramalho, Pimenta, Paulo Filemon Paolucci
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8931
Resumo: Universidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, Brazil
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spelling Martins, Gustavo FerreiraGuedes, Bruno Augusto MacielSilva, Luciana MariaSerrão, José EduardoDias, Consuelo Latorre FortesOrtigão, José Marcelo RamalhoPimenta, Paulo Filemon Paolucci2014-11-21T13:18:44Z2014-11-21T13:18:44Z2011MARTINS, Gustavo Ferreira et al. Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae. Tissue & Cell, v. 43, n. 2, p. 83-90, 2011.0040-8166https://www.arca.fiocruz.br/handle/icict/893110.1016/j.tice.2010.12.003engOliver & Boyd.Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupaeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brazil/Universidades Federal de Minas Gerais. Departamento de Morfologia. Belo Horizonte, MG, BrazilFundação Ezequiel Dias. Laboratório de Biologia Celular. Belo Horizonte, MG, BrazilUniversidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, BrazilFundação Ezequiel Dias. Laboratório de Biologia Celular. Belo Horizonte, MG, BrazilKansas State University. Department of Entomology. Manhattan, KS, USAFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilOenocytes are ectodermic cells that participate in a number of critical physiological roles such as detoxification and lipid storage and metabolism in insects. In light of the lack of information on oenocytes from Aedes aegypti and the potential role of these cells in the biology of this major yellow fever and dengue vector, we developed a protocol to purify and maintain Ae. aegypti pupa oenocytes in primary culture. Ae. aegypti oenocytes were cultured as clustered and as isolated ovoid cells with a smooth surface. Our results demonstrate that these cells remain viable in cell culture for at least two months. We also investigated their morphology in vivo and in vitro using light, confocal, scanning and transmission electron microscopes. This work is the first successful attempt in isolating and maintaining Ae. aegypti oenocytes in culture, and a significant step towards understanding the role of this cell type in this important disease vector. The purification and the development of primary cultures of insect oenocytes will allow future studies of their metabolism in producing and secreting compounds.Aedes aegyptiOenocytePrimary cultureMorphologyMosquitoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8931/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALIsolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.pdfIsolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.pdfapplication/pdf2357336https://www.arca.fiocruz.br/bitstream/icict/8931/2/Isolation%2c%20primary%20culture%20and%20morphological%20characterization%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdf02ef6b7fd0c499fc9833943fcbb76996MD52TEXTIsolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.pdf.txtIsolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae.pdf.txtExtracted texttext/plain31047https://www.arca.fiocruz.br/bitstream/icict/8931/3/Isolation%2c%20primary%20culture%20and%20morphological%20characterization%20of%20oenocytes%20from%20Aedes%20aegypti%20pupae.pdf.txtd5aa872fb9aed5346b9ce22b0e61d0daMD53icict/89312019-06-19 10:11:47.255oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:11:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
title Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
spellingShingle Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
Martins, Gustavo Ferreira
Aedes aegypti
Oenocyte
Primary culture
Morphology
Mosquito
title_short Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
title_full Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
title_fullStr Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
title_full_unstemmed Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
title_sort Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae
author Martins, Gustavo Ferreira
author_facet Martins, Gustavo Ferreira
Guedes, Bruno Augusto Maciel
Silva, Luciana Maria
Serrão, José Eduardo
Dias, Consuelo Latorre Fortes
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
author_role author
author2 Guedes, Bruno Augusto Maciel
Silva, Luciana Maria
Serrão, José Eduardo
Dias, Consuelo Latorre Fortes
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Martins, Gustavo Ferreira
Guedes, Bruno Augusto Maciel
Silva, Luciana Maria
Serrão, José Eduardo
Dias, Consuelo Latorre Fortes
Ortigão, José Marcelo Ramalho
Pimenta, Paulo Filemon Paolucci
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Oenocyte
Primary culture
Morphology
Mosquito
topic Aedes aegypti
Oenocyte
Primary culture
Morphology
Mosquito
description Universidade Federal de Viçosa. Departamento de Biologia Geral. Laboratório de Biologia Celular. Viçosa, MG, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-21T13:18:44Z
dc.date.available.fl_str_mv 2014-11-21T13:18:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARTINS, Gustavo Ferreira et al. Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae. Tissue & Cell, v. 43, n. 2, p. 83-90, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8931
dc.identifier.issn.none.fl_str_mv 0040-8166
dc.identifier.doi.none.fl_str_mv 10.1016/j.tice.2010.12.003
identifier_str_mv MARTINS, Gustavo Ferreira et al. Isolation, primary culture and morphological characterization of oenocytes from Aedes aegypti pupae. Tissue & Cell, v. 43, n. 2, p. 83-90, 2011.
0040-8166
10.1016/j.tice.2010.12.003
url https://www.arca.fiocruz.br/handle/icict/8931
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oliver & Boyd.
publisher.none.fl_str_mv Oliver & Boyd.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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