A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota

Detalhes bibliográficos
Autor(a) principal: Buarque, Diego de Souza
Data de Publicação: 2016
Outros Autores: Gomes, Cícera Maria, Araújo, Ricardo Nascimento, Pereira, Marcos Horacio, Ferreira, Roberta Carvalho, Guarneri, Alessandra Aparecida, Tanaka, Aparecida Sadae
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16136
Resumo: 2021-01-01
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spelling Buarque, Diego de SouzaGomes, Cícera MariaAraújo, Ricardo NascimentoPereira, Marcos HoracioFerreira, Roberta CarvalhoGuarneri, Alessandra AparecidaTanaka, Aparecida Sadae2016-10-06T17:29:00Z2016-10-06T17:29:00Z2016BUARQUE, Diego de Souza et al. A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota. Biochimie., vol. 123, p. 138-143, 2016.0300-9084https://www.arca.fiocruz.br/handle/icict/1613610.1016/j.biochi.2016.02.009engElsevier B.V.Propriedades antimicrobianasAntimicrobial propertiesBacteriaMidgutParasiteTriatoma infestansTrypanosoma cruziA new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiotainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2021-01-01Universidade Federal de Sao Paulo. Escola Paulista de Medicina. Departamento de Bioquimica. Sao Paulo, SP, BrasilUniversidade Federal de Sao Paulo. Escola Paulista de Medicina. Departamento de Bioquimica. Sao Paulo, SP, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Departamento de Parasitologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Sao Paulo. Escola Paulista de Medicina. Departamento de Bioquimica. Sao Paulo, SP, BrasilThe Reduviid Triatoma infestans is a vector for the protozoan Trypanosoma cruzi, the etiological agent of Chagas disease. The parasite must address the defense molecules and microbiota that colonize the anterior midgut of T. infestans. To obtain insight into T. cruzi - microbiota interactions in triatomine insects, we characterized a new antimicrobial product from the anterior midgut of T. infestans (TiAP) that may be involved in these relationships. The TiAP DNA fragment was cloned and expressed in a bacterial system, and the effect of the protein on bacteria and T. cruzi was evaluated by RNAi, qPCR and antimicrobial experiments. The number of T. cruzi in T. infestans anterior midguts was significantly lower in TiAP knockdown insects than in unsilenced groups. We also verified that the amount of bacteria in silenced T. infestans is approximately 600-fold higher than in unsilenced insects by qPCR. The 327-bp cDNA fragment that encodes mature TiAP was cloned into the pET-14b vector and expressed fused to a His-tag in Escherichia coli C43. The recombinant protein (rTiAP) was purified using an Ni-NTA column, followed by a HiTrap SP column. According to a trypanocidal assay, rTiAP did not interfere with the viability of T. cruzi trypomastigotes. Moreover, in antimicrobial experiments using E. coli and Micrococcus luteus, the protein was only bacteriostatic for Gram-negative bacteria. The data indicate that infection by T. cruzi increases the expression of TiAP to modulate the microbiota. The inhibition of microbiota growth by TiAP is important for parasite establishment in the T. infestans anterior midgut.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16136/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALve_Buarque_Diego_A new antimicrobial_CPqRR_2016.pdfve_Buarque_Diego_A new antimicrobial_CPqRR_2016.pdfapplication/pdf796289https://www.arca.fiocruz.br/bitstream/icict/16136/2/ve_Buarque_Diego_A%20new%20antimicrobial_CPqRR_2016.pdf951f9d823fc4efcfda380cf7c811515aMD52TEXTve_Buarque_Diego_A new antimicrobial_CPqRR_2016.pdf.txtve_Buarque_Diego_A new antimicrobial_CPqRR_2016.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
title A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
spellingShingle A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
Buarque, Diego de Souza
Propriedades antimicrobianas
Antimicrobial properties
Bacteria
Midgut
Parasite
Triatoma infestans
Trypanosoma cruzi
title_short A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
title_full A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
title_fullStr A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
title_full_unstemmed A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
title_sort A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota
author Buarque, Diego de Souza
author_facet Buarque, Diego de Souza
Gomes, Cícera Maria
Araújo, Ricardo Nascimento
Pereira, Marcos Horacio
Ferreira, Roberta Carvalho
Guarneri, Alessandra Aparecida
Tanaka, Aparecida Sadae
author_role author
author2 Gomes, Cícera Maria
Araújo, Ricardo Nascimento
Pereira, Marcos Horacio
Ferreira, Roberta Carvalho
Guarneri, Alessandra Aparecida
Tanaka, Aparecida Sadae
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Buarque, Diego de Souza
Gomes, Cícera Maria
Araújo, Ricardo Nascimento
Pereira, Marcos Horacio
Ferreira, Roberta Carvalho
Guarneri, Alessandra Aparecida
Tanaka, Aparecida Sadae
dc.subject.other.pt_BR.fl_str_mv Propriedades antimicrobianas
topic Propriedades antimicrobianas
Antimicrobial properties
Bacteria
Midgut
Parasite
Triatoma infestans
Trypanosoma cruzi
dc.subject.en.pt_BR.fl_str_mv Antimicrobial properties
Bacteria
Midgut
Parasite
Triatoma infestans
Trypanosoma cruzi
description 2021-01-01
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-10-06T17:29:00Z
dc.date.available.fl_str_mv 2016-10-06T17:29:00Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BUARQUE, Diego de Souza et al. A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota. Biochimie., vol. 123, p. 138-143, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16136
dc.identifier.issn.pt_BR.fl_str_mv 0300-9084
dc.identifier.eissn.none.fl_str_mv 10.1016/j.biochi.2016.02.009
identifier_str_mv BUARQUE, Diego de Souza et al. A new antimicrobial protein from the anterior midgut of Triatoma infestans mediates Trypanosoma cruzi establishment by controlling the microbiota. Biochimie., vol. 123, p. 138-143, 2016.
0300-9084
10.1016/j.biochi.2016.02.009
url https://www.arca.fiocruz.br/handle/icict/16136
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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