Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/57249 |
Resumo: | Eprint version - Post-print |
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Silva, Mariana Barbalho Farias daMota, Fabio FariaJurelevicius, DiogoAzevedo, Vasco Ariston de CarvalhoCosta, Mateus Matiuzzi daGóes Neto, AristótelesRamos, Rommel Thiago JucáCastro Soares, Siomar deRosado, Alexandre S.Seldin, Lucy2023-03-06T19:19:30Z2023-03-06T19:19:30Z2022SILVA, Mariana Barbalho Faria da et al. Genomic analyses of a novel bioemulsifier-producing Psychrobacillus strain isolated from soil of King George Island, Antarctica. Polar Biology, p. 1 - 27, 2023.0722-4060https://www.arca.fiocruz.br/handle/icict/57249Eprint version - Post-printUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal do Vale do São Francisco. Petrolina, BA, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal do Pará. Instituto de Ciências Biológicas. Pará. Brasil.Universidade Federal do Triângulo Mineiro. Instituto de Ciências Biológicas e Naturais. Uberaba, MG, Brasil.KAUST, King 15 Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Cold-adapted bacterial strains are potentially valuable for biotechnological applications 27 involving the production of cold-active enzymes and bioproducts important to various 28 industries. A psychrotolerant, aerobic, Gram-positive, endospore-forming, 29 bioemulsifier-producing strain, named Val9, was isolated from Vale Ulman soil 30 samples, King George Island, Antarctica and identified as a member of the genus 31 Psychrobacillus. To better characterize this novel strain, its whole genome was 32 sequenced revealing a size of 3,986,526 bp with a G+C content of 36.6 %, and 4,042 33 predicted coding DNA sequences (CDSs). Digital DNA-DNA hybridization (dDDH) 34 and average nucleotide identity (ANI) analyses between strain Val9 and the type strains 35 of the seven Psychrobacillus species revealed that the highest values were observed 36 with Psychrobacillus psychrodurans DSM11713T but below the conventional 37 thresholds of 70 % dDDH and 95 % ANI for bacterial species assignment, suggesting 38 that strain Val9 could represent a distinct species. As potential low-temperature 39 adaptation strategies, genes encoding cold-shock proteins, transporters for glycine 40 betaine, carnitine and choline, and enzymes acting against oxidative stress were found 41 in Val9 genome. DEAD-box RNA helicases, important for cold and oxidative tolerance, 42 and a two-component signal transduction system related to plasmatic membrane fluidity 43 as well as biotechnologically important CDSs, related to levan production, were 44 detected. The sacB gene encoding the enzyme levansucrase was exclusive for Val9 and 45 it was not found in the other Psychrobacillus type strains. Altogether, the comparative 46 genomic analyses presented here highlight important metabolic pathways and the 47 biotechnological potential of this novel strain.engSpringer Science and Business Media LLCPsychrobacillusGenomaAntarcticaBioemulsificanteAdaptação de baixa temperaturaPsychrobacillusGenomeAntarcticaBioemulsifierLow-temperature adaptationGenomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarcticainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/57249/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFabioFaria_etal_IOC_2023.pdfFabioFaria_etal_IOC_2023.pdfapplication/pdf669415https://www.arca.fiocruz.br/bitstream/icict/57249/2/FabioFaria_etal_IOC_2023.pdf254477378e58f9b0354b9a6a415d9823MD52icict/572492023-09-04 11:30:09.907oai:www.arca.fiocruz.br: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ório 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| dc.title.en_US.fl_str_mv |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| title |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| spellingShingle |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica Silva, Mariana Barbalho Farias da Psychrobacillus Genoma Antarctica Bioemulsificante Adaptação de baixa temperatura Psychrobacillus Genome Antarctica Bioemulsifier Low-temperature adaptation |
| title_short |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| title_full |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| title_fullStr |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| title_full_unstemmed |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| title_sort |
Genomic analyses of a novel bioemulsifierproducing Psychrobacillus strain isolated from soil of King George Island, Antarctica |
| author |
Silva, Mariana Barbalho Farias da |
| author_facet |
Silva, Mariana Barbalho Farias da Mota, Fabio Faria Jurelevicius, Diogo Azevedo, Vasco Ariston de Carvalho Costa, Mateus Matiuzzi da Góes Neto, Aristóteles Ramos, Rommel Thiago Jucá Castro Soares, Siomar de Rosado, Alexandre S. Seldin, Lucy |
| author_role |
author |
| author2 |
Mota, Fabio Faria Jurelevicius, Diogo Azevedo, Vasco Ariston de Carvalho Costa, Mateus Matiuzzi da Góes Neto, Aristóteles Ramos, Rommel Thiago Jucá Castro Soares, Siomar de Rosado, Alexandre S. Seldin, Lucy |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Mariana Barbalho Farias da Mota, Fabio Faria Jurelevicius, Diogo Azevedo, Vasco Ariston de Carvalho Costa, Mateus Matiuzzi da Góes Neto, Aristóteles Ramos, Rommel Thiago Jucá Castro Soares, Siomar de Rosado, Alexandre S. Seldin, Lucy |
| dc.subject.other.en_US.fl_str_mv |
Psychrobacillus Genoma Antarctica Bioemulsificante Adaptação de baixa temperatura |
| topic |
Psychrobacillus Genoma Antarctica Bioemulsificante Adaptação de baixa temperatura Psychrobacillus Genome Antarctica Bioemulsifier Low-temperature adaptation |
| dc.subject.en.en_US.fl_str_mv |
Psychrobacillus Genome Antarctica Bioemulsifier Low-temperature adaptation |
| description |
Eprint version - Post-print |
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2022 |
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2022 |
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2023-03-06T19:19:30Z |
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2023-03-06T19:19:30Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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SILVA, Mariana Barbalho Faria da et al. Genomic analyses of a novel bioemulsifier-producing Psychrobacillus strain isolated from soil of King George Island, Antarctica. Polar Biology, p. 1 - 27, 2023. |
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https://www.arca.fiocruz.br/handle/icict/57249 |
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0722-4060 |
| identifier_str_mv |
SILVA, Mariana Barbalho Faria da et al. Genomic analyses of a novel bioemulsifier-producing Psychrobacillus strain isolated from soil of King George Island, Antarctica. Polar Biology, p. 1 - 27, 2023. 0722-4060 |
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https://www.arca.fiocruz.br/handle/icict/57249 |
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eng |
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eng |
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Springer Science and Business Media LLC |
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Springer Science and Business Media LLC |
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