Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/16629 |
Resumo: | Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia para a Inovação em Doenças Negligenciadas (INCT-IDN). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Produtos Farmacêuticos. Rio de Janeiro, RJ, Brasil. |
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Souza, André L. A.Faria, Robson X.Calabrese, Kátia S.Hardoim, Daiana de JesusTaniwaki, NoemiAlves, Luiz A.De Simone, Salvatore Giovanni2017-01-03T10:15:50Z2017-01-03T10:15:50Z2016SOUZA, André L. A. et al. Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi. Plos One, v.11, n.7, 30157673, 20p, July 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1662910.1371/journal. pone.0157673engPublic Library of ScienceTrypanosoma cruziDoença de ChagasTemporizinPeptídeos antimicrobianostemporizin-1Trypanosoma cruziChagas DiseaseTemporizinAntimicrobial peptidestemporizin-1Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia para a Inovação em Doenças Negligenciadas (INCT-IDN). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Produtos Farmacêuticos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz. Laboratório de Toxoplasmose e outras Protozoonoses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Adolfo Lutz. Seção de Microscopia Eletrônica. Araçatuba, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Rio de Janeiro, RJ. Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Tropical diseases caused by parasitic infections continue to cause socioeconomic distress worldwide. Among these, Chagas disease has become a great concern because of globalization. Caused by Trypanosoma cruzi, there is an increasing need to discover new, more effective methods to manage infections that minimize disease onset. Antimicrobial peptides represent a possible solution to this challenge. As effector molecules of the innate immune response against pathogens, they are the first line of defense found in all multi-cellular organisms. In amphibians, temporins are a large family of antimicrobial peptides found in skin secretions. Their functional roles and modes of action present unique properties that indicate possible candidates for therapeutic applications. Here, we investigated the trypanocide activity of temporizin and temporizin-1. Temporizin is an artificial, hybrid peptide containing the N-terminal region of temporin A, the pore-forming region of gramicidin and a Cterminus consisting of alternating leucine and lysine. Temporizin-1 is a modification of temporizin with a reduction in the region responsible for insertion into membranes. Their activities were evaluated in a cell permeabilization assay by flow cytometry, an LDH release assay, electron microscopy, an MTT assay and patch clamp experiments. Both temporizin and temporizin-1 demonstrated toxicity against T. cruzi with temporizin displaying slightly more potency. At concentrations up to 100 μg/ ml, both peptides exhibited low toxicity in J774 cells, a macrophage lineage cell line, and no toxicity was observed in mouse primary peritoneal macrophages. In contrast, the peptides showed some toxicity in rat adenoma GH3 cells and Jurkat human lymphoma cells with temporizin-1 displaying lower toxicity. In summary, a shortened form of the hybrid temporizin peptide, temporizin-1, was efficient at killing T. cruzi and it has low toxicity in wild-type mammalian cells. These data suggest that temporizin-1 might be a candidate for Chagas disease therapy.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16629/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALkatia_calabrese_etal_IOC_2016.pdfkatia_calabrese_etal_IOC_2016.pdfapplication/pdf3404748https://www.arca.fiocruz.br/bitstream/icict/16629/2/katia_calabrese_etal_IOC_2016.pdffb6b61ca57828307152d5ab9081cb4d4MD52TEXTkatia_calabrese_etal_IOC_2016.pdf.txtkatia_calabrese_etal_IOC_2016.pdf.txtExtracted texttext/plain69147https://www.arca.fiocruz.br/bitstream/icict/16629/3/katia_calabrese_etal_IOC_2016.pdf.txt8051efbe67071d23121038a5091d2fd2MD53icict/166292023-09-04 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| dc.title.pt_BR.fl_str_mv |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| title |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| spellingShingle |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi Souza, André L. A. Trypanosoma cruzi Doença de Chagas Temporizin Peptídeos antimicrobianos temporizin-1 Trypanosoma cruzi Chagas Disease Temporizin Antimicrobial peptides temporizin-1 |
| title_short |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| title_full |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| title_fullStr |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| title_full_unstemmed |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| title_sort |
Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi |
| author |
Souza, André L. A. |
| author_facet |
Souza, André L. A. Faria, Robson X. Calabrese, Kátia S. Hardoim, Daiana de Jesus Taniwaki, Noemi Alves, Luiz A. De Simone, Salvatore Giovanni |
| author_role |
author |
| author2 |
Faria, Robson X. Calabrese, Kátia S. Hardoim, Daiana de Jesus Taniwaki, Noemi Alves, Luiz A. De Simone, Salvatore Giovanni |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza, André L. A. Faria, Robson X. Calabrese, Kátia S. Hardoim, Daiana de Jesus Taniwaki, Noemi Alves, Luiz A. De Simone, Salvatore Giovanni |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma cruzi Doença de Chagas Temporizin Peptídeos antimicrobianos temporizin-1 |
| topic |
Trypanosoma cruzi Doença de Chagas Temporizin Peptídeos antimicrobianos temporizin-1 Trypanosoma cruzi Chagas Disease Temporizin Antimicrobial peptides temporizin-1 |
| dc.subject.en.pt_BR.fl_str_mv |
Trypanosoma cruzi Chagas Disease Temporizin Antimicrobial peptides temporizin-1 |
| description |
Fundação Oswaldo Cruz. Centro de Desenvolvimento Tecnológico em Saúde (CDTS). Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia para a Inovação em Doenças Negligenciadas (INCT-IDN). Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica Experimental e Computacional de Produtos Farmacêuticos. Rio de Janeiro, RJ, Brasil. |
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2016 |
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2016 |
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2017-01-03T10:15:50Z |
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2017-01-03T10:15:50Z |
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info:eu-repo/semantics/article |
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SOUZA, André L. A. et al. Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi. Plos One, v.11, n.7, 30157673, 20p, July 2016. |
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https://www.arca.fiocruz.br/handle/icict/16629 |
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1932-6203 |
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10.1371/journal. pone.0157673 |
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SOUZA, André L. A. et al. Temporizin and Temporizin-1 Peptides as Novel Candidates for Eliminating Trypanosoma cruzi. Plos One, v.11, n.7, 30157673, 20p, July 2016. 1932-6203 10.1371/journal. pone.0157673 |
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