Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil

Detalhes bibliográficos
Autor(a) principal: Barreira, Thais
Data de Publicação: 2022
Outros Autores: Moreira, Danielly Corrêa, Borba, Cintia, Moraes, Aurea, Oliveira, Manoel
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/52336
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infeciosas. Evandro Chagas. Laboratório Multiusuário da instalação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Pesquisa Clínica em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
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spelling Barreira, ThaisMoreira, Danielly CorrêaBorba, CintiaMoraes, AureaOliveira, Manoel2022-04-18T20:32:16Z2022-04-18T20:32:16Z2022BARREIRA, Thais et al. Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil. Current Research in Microbial Sciences, v. 3, 100128, p. 1-7, 2022.2666-5174https://www.arca.fiocruz.br/handle/icict/5233610.1016/j.crmicr.2022.100128engElsevierColeção de culturaViabilidadeEstabilidade morfofisiológicaIdentificação molecular e β-tubulinaIdentificação polifásicaSporothrix schenckiiEsporotricoseCulture collectionViabilityMorphophysiological stabilityMolecular identification and β-tubulinPolyphasic identificationSporothrix schenckiiSporotrichosisMolecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Doenças Infeciosas. Evandro Chagas. Laboratório Multiusuário da instalação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Pesquisa Clínica em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Pesquisa Clínica em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Prospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Prospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Prospecção de Fungos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Taxonomia, Bioquímica e Prospecção de Fungos. Rio de Janeiro, RJ, Brasil / Plataforma Internacional de Ciência, Tecnologia e Inovação em Saúde. Rio de Janeiro, RJ, Brasil.Many isolates from patients with sporotrichosis are preserved in culture collections by different methods around the world. The preservation methods are used to maintain the viability and the morphophysiological and genetic characteristics of isolates for long periods. In this study, we evaluated 34 isolates, previously, identified as S. schenckii by a classical identification method, initially preserved by periodical subcultures and then under mineral oil at culture collection of Oswaldo Cruz Institute/Fiocruz, to re-identify them by polyphasic identification. Our results showed that seven isolates remained viable for 34 to 64 years under oil, one isolate lost the ability to sporulate which was reverted by using a medium culture supplemented with rosebush branches and all of them were identified as Sporothrix schenckii sensu stricto by morphological, physiological, partial β-tubulin gene sequencing and phylogenetic analysis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/52336/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALThaisBarreira_AureaMoraes_etal_IOC_2022.pdfThaisBarreira_AureaMoraes_etal_IOC_2022.pdfapplication/pdf8550544https://www.arca.fiocruz.br/bitstream/icict/52336/2/ThaisBarreira_AureaMoraes_etal_IOC_2022.pdfe45fb1e549fd8bd88da39ff6fa734693MD52icict/523362022-04-25 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dc.title.pt_BR.fl_str_mv Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
title Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
spellingShingle Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
Barreira, Thais
Coleção de cultura
Viabilidade
Estabilidade morfofisiológica
Identificação molecular e β-tubulina
Identificação polifásica
Sporothrix schenckii
Esporotricose
Culture collection
Viability
Morphophysiological stability
Molecular identification and β-tubulin
Polyphasic identification
Sporothrix schenckii
Sporotrichosis
title_short Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
title_full Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
title_fullStr Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
title_full_unstemmed Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
title_sort Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil
author Barreira, Thais
author_facet Barreira, Thais
Moreira, Danielly Corrêa
Borba, Cintia
Moraes, Aurea
Oliveira, Manoel
author_role author
author2 Moreira, Danielly Corrêa
Borba, Cintia
Moraes, Aurea
Oliveira, Manoel
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Barreira, Thais
Moreira, Danielly Corrêa
Borba, Cintia
Moraes, Aurea
Oliveira, Manoel
dc.subject.other.pt_BR.fl_str_mv Coleção de cultura
Viabilidade
Estabilidade morfofisiológica
Identificação molecular e β-tubulina
Identificação polifásica
Sporothrix schenckii
Esporotricose
topic Coleção de cultura
Viabilidade
Estabilidade morfofisiológica
Identificação molecular e β-tubulina
Identificação polifásica
Sporothrix schenckii
Esporotricose
Culture collection
Viability
Morphophysiological stability
Molecular identification and β-tubulin
Polyphasic identification
Sporothrix schenckii
Sporotrichosis
dc.subject.en.pt_BR.fl_str_mv Culture collection
Viability
Morphophysiological stability
Molecular identification and β-tubulin
Polyphasic identification
Sporothrix schenckii
Sporotrichosis
description Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infeciosas. Evandro Chagas. Laboratório Multiusuário da instalação. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Pesquisa Clínica em Doenças Infecciosas. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-04-18T20:32:16Z
dc.date.available.fl_str_mv 2022-04-18T20:32:16Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BARREIRA, Thais et al. Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil. Current Research in Microbial Sciences, v. 3, 100128, p. 1-7, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/52336
dc.identifier.issn.pt_BR.fl_str_mv 2666-5174
dc.identifier.doi.none.fl_str_mv 10.1016/j.crmicr.2022.100128
identifier_str_mv BARREIRA, Thais et al. Molecular and phenotypic reidentification of Sporothrix schenckii clinical isolates preserved under mineral oil for 34 to 64 years in a culture collection in Brazil. Current Research in Microbial Sciences, v. 3, 100128, p. 1-7, 2022.
2666-5174
10.1016/j.crmicr.2022.100128
url https://www.arca.fiocruz.br/handle/icict/52336
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/52336/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/52336/2/ThaisBarreira_AureaMoraes_etal_IOC_2022.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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