Laboratory Mice Euthanasia: Speed Death and Animal Welfare

Detalhes bibliográficos
Autor(a) principal: Brito, Cleiton Felizardo de
Data de Publicação: 2020
Outros Autores: Evangelista, Alexsandre Andrade, Felippe, Renata Machado, Cascabulho, Cynthia, Fragoso, Viviane Muniz, Oliveira, Gabriel Melo de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42534
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
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spelling Brito, Cleiton Felizardo deEvangelista, Alexsandre AndradeFelippe, Renata MachadoCascabulho, CynthiaFragoso, Viviane MunizOliveira, Gabriel Melo de2020-08-04T18:34:40Z2020-08-04T18:34:40Z2020BRITO, Cleiton Felizardo de et al. Laboratory Mice Euthanasia: Speed Death and Animal Welfare. American Journal of Biomedical Science & Research, v. 8, n. 4, 15p, Apr. 2020.2642-1747https://www.arca.fiocruz.br/handle/icict/4253410.34297/AJBSR.2020.08.001300engBiomedGrid LLCEutanásiaBem-EstarRatoAgentes de inalação e anestésicos injetáveisEuthanasiaWelfareMouseInhalation Agents and Injectable AnestheticsLaboratory Mice Euthanasia: Speed Death and Animal Welfareinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.The euthanasia is one of the most studied, debated and controversial topics in the laboratory animal science area. All over the world, the ideal way to perform euthanasia in laboratory animals is sought, seeking, in summary, three main points, animal welfare during the procedure; minimal influence on the animals biological system, not to interfere with test results and accessibility and low cost. In Brazil, after the validity of Law No 11.794-2008, that regulated the use of animals for didactic and scientific purposes, structuring the Animal Experimentation Control Council (CONCEA). In its resolutions, CONCEA standardized the application of accepted euthanasia methods, mainly overdose of injectable anesthetics and restricted use of carbon dioxide (CO2 ). The aim of our study was to evaluate within the rules of CONCEA and using non-invasive and invasive parameters which would be the best euthanasia promoter in adult Swiss Webster male mice. We compared the use of CO2 (suggested a substitution in the in sufflation valve) Isoflurane saturation, a combination of induction in an environment saturated with isoflurane and progressive CO2 in sufflation and application of the association Ketamin and Xilazin, in overdose. Our results, clearly, demonstrate that the most humane method of inducing the death of the animal is that which is the fastest (Speed Death); does not promote pain, discomfort or distress in the animal; minimally influences the biological systems of the animals and has a low acquisition cost. From our results it was possible to suggest that the combination of isoflurane (5%) and CO2 (1 L/min) is the most efficient methodology for performing euthanasia in mice. Because it is extremely fast, mice does not show discomfort, does not influence the biological systems of the animal after death (except the Central Nervous System) and has a low cost with relative ease of access. Thus, we believe that with this study, we can demonstrate that through projects, studies and tests we can suggest new and reliable methodologies for the application of the welfare of laboratory animals.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42534/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCleitonFBrito_VivianeMFragoso_etal_IOC_2020.pdfCleitonFBrito_VivianeMFragoso_etal_IOC_2020.pdfapplication/pdf1113411https://www.arca.fiocruz.br/bitstream/icict/42534/2/CleitonFBrito_VivianeMFragoso_etal_IOC_2020.pdfa7a7a2d1b9084c651ade0f6ec84c2a74MD52TEXTCleitonFBrito_VivianeMFragoso_etal_IOC_2020.pdf.txtCleitonFBrito_VivianeMFragoso_etal_IOC_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Laboratory Mice Euthanasia: Speed Death and Animal Welfare
title Laboratory Mice Euthanasia: Speed Death and Animal Welfare
spellingShingle Laboratory Mice Euthanasia: Speed Death and Animal Welfare
Brito, Cleiton Felizardo de
Eutanásia
Bem-Estar
Rato
Agentes de inalação e anestésicos injetáveis
Euthanasia
Welfare
Mouse
Inhalation Agents and Injectable Anesthetics
title_short Laboratory Mice Euthanasia: Speed Death and Animal Welfare
title_full Laboratory Mice Euthanasia: Speed Death and Animal Welfare
title_fullStr Laboratory Mice Euthanasia: Speed Death and Animal Welfare
title_full_unstemmed Laboratory Mice Euthanasia: Speed Death and Animal Welfare
title_sort Laboratory Mice Euthanasia: Speed Death and Animal Welfare
author Brito, Cleiton Felizardo de
author_facet Brito, Cleiton Felizardo de
Evangelista, Alexsandre Andrade
Felippe, Renata Machado
Cascabulho, Cynthia
Fragoso, Viviane Muniz
Oliveira, Gabriel Melo de
author_role author
author2 Evangelista, Alexsandre Andrade
Felippe, Renata Machado
Cascabulho, Cynthia
Fragoso, Viviane Muniz
Oliveira, Gabriel Melo de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brito, Cleiton Felizardo de
Evangelista, Alexsandre Andrade
Felippe, Renata Machado
Cascabulho, Cynthia
Fragoso, Viviane Muniz
Oliveira, Gabriel Melo de
dc.subject.other.pt_BR.fl_str_mv Eutanásia
Bem-Estar
Rato
Agentes de inalação e anestésicos injetáveis
topic Eutanásia
Bem-Estar
Rato
Agentes de inalação e anestésicos injetáveis
Euthanasia
Welfare
Mouse
Inhalation Agents and Injectable Anesthetics
dc.subject.en.pt_BR.fl_str_mv Euthanasia
Welfare
Mouse
Inhalation Agents and Injectable Anesthetics
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-04T18:34:40Z
dc.date.available.fl_str_mv 2020-08-04T18:34:40Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRITO, Cleiton Felizardo de et al. Laboratory Mice Euthanasia: Speed Death and Animal Welfare. American Journal of Biomedical Science & Research, v. 8, n. 4, 15p, Apr. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42534
dc.identifier.issn.pt_BR.fl_str_mv 2642-1747
dc.identifier.doi.none.fl_str_mv 10.34297/AJBSR.2020.08.001300
identifier_str_mv BRITO, Cleiton Felizardo de et al. Laboratory Mice Euthanasia: Speed Death and Animal Welfare. American Journal of Biomedical Science & Research, v. 8, n. 4, 15p, Apr. 2020.
2642-1747
10.34297/AJBSR.2020.08.001300
url https://www.arca.fiocruz.br/handle/icict/42534
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BiomedGrid LLC
publisher.none.fl_str_mv BiomedGrid LLC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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