Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification

Detalhes bibliográficos
Autor(a) principal: Trilles, Luciana
Data de Publicação: 2014
Outros Autores: Wang, Bin, Firacative, Carolina, Lazéra, Márcia dos Santos, Wanke, Bodo, Meyer, Wieland
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9940
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.
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spelling Trilles, LucianaWang, BinFiracative, CarolinaLazéra, Márcia dos SantosWanke, BodoMeyer, Wieland2015-04-09T16:51:37Z2015-04-09T16:51:37Z2014TRILLES, Luciana et al. Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification. Plos One, v. 9, n. 4, p. 1-8, Apr. 2014.1932-6203https://www.arca.fiocruz.br/handle/icict/994010.1371/journal.pone.00946481932-6203porPublic Library of ScienceIdentification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplificationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.University of Sydney. Westmead Millennium Institute. Centre for Virus Research, Retroviral Genetic Laboratory. Sydney, Australia.The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.The agents of cryptococcosis C. neoformans and C. gattii are important agents of meningoencephalitis in immunocompromised and immunocompetent hosts, respectively. They are grouped into eight major molecular types, VNI-VNIV for C. neoformans and VGI-VGIV for C. gattii. These major molecular types differ in their host range, epidemiology, antifungal susceptibility and geographic distribution. To enable a rapid identification of the major molecular types and potential hybrids within the two species specific probes based on the PLB1 gene in combination with hyperbranched rolling circle amplification (HRCA) were developed. HRCA was applied to 76 cryptococcal strains, 10 strains each representing the 7 haploid major molecular types, 4 VNIII hybrid strains and 2 inter-species hybrid strains. All strains were correctly identified to the major molecular type and or hybrid type using HRCA alone. To increase the sensitivity a semi-nested PCR step was developed, which will enable the identification of the molecular types/hybrids directly from clinical samples, harboring a low copy number of DNA (40 copies). Thus, HRCA based on the PLB1 locus alone and in combination with a semi-nested PCR showed to be a specific and sensitive methodology, with a great potential to be used on clinical specimens for the direct diagnosis of the agents of cryptococcosis, including hybrid strains, enabling a rapid and patient tailored treatment choice of this disease.Cryptococcus neoformansCryptococcus gattiiMeningoencephalitisPolymerase chain reactioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9940/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALIdentification of the major molecular types of cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification.pdfIdentification of the major molecular types of cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification.pdfapplication/pdf771408https://www.arca.fiocruz.br/bitstream/icict/9940/2/Identification%20of%20the%20major%20molecular%20types%20of%20cryptococcus%20neoformans%20and%20C.%20gattii%20by%20hyperbranched%20rolling%20circle%20amplification.pdf288a2af3227a224716851954e9340ebfMD52TEXTIdentification of the major molecular types of cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification.pdf.txtIdentification of the major molecular types of cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification.pdf.txtExtracted texttext/plain39918https://www.arca.fiocruz.br/bitstream/icict/9940/3/Identification%20of%20the%20major%20molecular%20types%20of%20cryptococcus%20neoformans%20and%20C.%20gattii%20by%20hyperbranched%20rolling%20circle%20amplification.pdf.txt230e0bd01ffe165de1421e8e5645442aMD53icict/99402023-10-05 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dc.title.pt_BR.fl_str_mv Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
title Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
spellingShingle Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
Trilles, Luciana
Cryptococcus neoformans
Cryptococcus gattii
Meningoencephalitis
Polymerase chain reaction
title_short Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
title_full Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
title_fullStr Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
title_full_unstemmed Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
title_sort Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification
author Trilles, Luciana
author_facet Trilles, Luciana
Wang, Bin
Firacative, Carolina
Lazéra, Márcia dos Santos
Wanke, Bodo
Meyer, Wieland
author_role author
author2 Wang, Bin
Firacative, Carolina
Lazéra, Márcia dos Santos
Wanke, Bodo
Meyer, Wieland
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Trilles, Luciana
Wang, Bin
Firacative, Carolina
Lazéra, Márcia dos Santos
Wanke, Bodo
Meyer, Wieland
dc.subject.decs.pt_BR.fl_str_mv Cryptococcus neoformans
Cryptococcus gattii
Meningoencephalitis
Polymerase chain reaction
topic Cryptococcus neoformans
Cryptococcus gattii
Meningoencephalitis
Polymerase chain reaction
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil / The University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Sydney Medical School. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-04-09T16:51:37Z
dc.date.available.fl_str_mv 2015-04-09T16:51:37Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TRILLES, Luciana et al. Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification. Plos One, v. 9, n. 4, p. 1-8, Apr. 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9940
dc.identifier.issn.none.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0094648
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv TRILLES, Luciana et al. Identification of the major molecular types of Cryptococcus neoformans and C. gattii by hyperbranched rolling circle amplification. Plos One, v. 9, n. 4, p. 1-8, Apr. 2014.
1932-6203
10.1371/journal.pone.0094648
url https://www.arca.fiocruz.br/handle/icict/9940
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/9940/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/9940/2/Identification%20of%20the%20major%20molecular%20types%20of%20cryptococcus%20neoformans%20and%20C.%20gattii%20by%20hyperbranched%20rolling%20circle%20amplification.pdf
https://www.arca.fiocruz.br/bitstream/icict/9940/3/Identification%20of%20the%20major%20molecular%20types%20of%20cryptococcus%20neoformans%20and%20C.%20gattii%20by%20hyperbranched%20rolling%20circle%20amplification.pdf.txt
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