Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion

Detalhes bibliográficos
Autor(a) principal: Conzentino, Marcelo S.
Data de Publicação: 2021
Outros Autores: Forchhammer, Karl, Souza, Emanuel Maltempi de, Pedrosa, Fábio O., Nogueira, Meri B., Raboni, Sônia M., Rego, Fabiane G. M., Zanette, Dalila Lucíola, Aoki, Mateus Nóbrega, Nardin, Jeanine M., Fornazari, Bruna, Morales, Hugo M. P., Celedon, Paola Alejandra Fiorani, Lima, Carla V. P., Mattar, Sibelle B., Lin, Vanessa H., Morello, Luis Gustavo, Marchini, Fabrício Klerynton, Reis, Rodrigo A., Huergo, Luciano F.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/49817
Resumo: Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
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spelling Conzentino, Marcelo S.Forchhammer, KarlSouza, Emanuel Maltempi dePedrosa, Fábio O.Nogueira, Meri B.Raboni, Sônia M.Rego, Fabiane G. M.Zanette, Dalila LucíolaAoki, Mateus NóbregaNardin, Jeanine M.Fornazari, BrunaMorales, Hugo M. P.Celedon, Paola Alejandra FioraniLima, Carla V. P.Mattar, Sibelle B.Lin, Vanessa H.Morello, Luis GustavoMarchini, Fabrício KleryntonReis, Rodrigo A.Huergo, Luciano F.2021-11-12T15:24:25Z2021-11-12T15:24:25Z2021CONZENTINO, Marcelo S. et al. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion. Brazilian Journal of Microbiology. p. 1–5, 2021.1678-4405https://www.arca.fiocruz.br/handle/icict/4981710.1007/s42770-021-00556-6porSociedade Brasileira de MicrobiologiaSARS-CoV-2COVID-19ELISAProteínas do NucleocapsídeoImmunologic TestsEnzyme-Linked Immunosorbent AssayNucleocapsid ProteinsPruebas InmunológicasEnsayo de Inmunoadsorción EnzimáticaProteínas de la NucleocápsideTests immunologiquesTest ELISAProtéines nucléocapsideTestes ImunológicosEnsaio de Imunoadsorção EnzimáticaAntigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Interfakultäres Institut für Mikrobiologie und Infektionsmedizin der Eberhard-Karls. Universität Tübingen. Tübingen, Germany.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil.Universidade Federal do Paraná. Hospital das Clínicas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Hospital das Clínicas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Programa de Pós-Graduação em Ciências Farmacêuticas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Hospital Erasto Gaertner. Curitiba, PR, Brasil.Hospital Erasto Gaertner. Curitiba, PR, Brasil.Hospital Erasto Gaertner. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.Serological assays are important tools to identify previous exposure to SARS-CoV-2, helping to track COVID-19 cases and determine the level of humoral response to SARS-CoV-2 infections and/or immunization to future vaccines. Here, the SARS-CoV-2 nucleocapsid protein was expressed in Escherichia coli and purifed to homogeneity and high yield using a single chromatography step. The purifed SARS-CoV-2 nucleocapsid protein was used to develop an indirect enzyme-linked immunosorbent assay for the identifcation of human SARS-CoV-2 seroconverts. The assay sensitivity and specifcity were determined analyzing sera from 140 RT-qPCR-confrmed COVID-19 cases and 210 pre-pandemic controls. The assay operated with 90% sensitivity and 98% specifcity; identical accuracies were obtained in head-to-head comparison with a commercial ELISA kit. Antigen-coated plates were stable for up to 3 months at 4 °C. The ELISA method described is ready for mass production and will be an additional tool to track COVID-19 cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/49817/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL42770_2021_Article_ok.pdf42770_2021_Article_ok.pdfapplication/pdf1504795https://www.arca.fiocruz.br/bitstream/icict/49817/2/42770_2021_Article_ok.pdf460c54d460a720625116ce13b176fca7MD52icict/498172021-12-23 16:31:19.229oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-12-23T19:31:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
title Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
spellingShingle Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
Conzentino, Marcelo S.
SARS-CoV-2
COVID-19
ELISA
Proteínas do Nucleocapsídeo
Immunologic Tests
Enzyme-Linked Immunosorbent Assay
Nucleocapsid Proteins
Pruebas Inmunológicas
Ensayo de Inmunoadsorción Enzimática
Proteínas de la Nucleocápside
Tests immunologiques
Test ELISA
Protéines nucléocapside
Testes Imunológicos
Ensaio de Imunoadsorção Enzimática
title_short Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
title_full Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
title_fullStr Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
title_full_unstemmed Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
title_sort Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion
author Conzentino, Marcelo S.
author_facet Conzentino, Marcelo S.
Forchhammer, Karl
Souza, Emanuel Maltempi de
Pedrosa, Fábio O.
Nogueira, Meri B.
Raboni, Sônia M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Fornazari, Bruna
Morales, Hugo M. P.
Celedon, Paola Alejandra Fiorani
Lima, Carla V. P.
Mattar, Sibelle B.
Lin, Vanessa H.
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Reis, Rodrigo A.
Huergo, Luciano F.
author_role author
author2 Forchhammer, Karl
Souza, Emanuel Maltempi de
Pedrosa, Fábio O.
Nogueira, Meri B.
Raboni, Sônia M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Fornazari, Bruna
Morales, Hugo M. P.
Celedon, Paola Alejandra Fiorani
Lima, Carla V. P.
Mattar, Sibelle B.
Lin, Vanessa H.
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Reis, Rodrigo A.
Huergo, Luciano F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Conzentino, Marcelo S.
Forchhammer, Karl
Souza, Emanuel Maltempi de
Pedrosa, Fábio O.
Nogueira, Meri B.
Raboni, Sônia M.
Rego, Fabiane G. M.
Zanette, Dalila Lucíola
Aoki, Mateus Nóbrega
Nardin, Jeanine M.
Fornazari, Bruna
Morales, Hugo M. P.
Celedon, Paola Alejandra Fiorani
Lima, Carla V. P.
Mattar, Sibelle B.
Lin, Vanessa H.
Morello, Luis Gustavo
Marchini, Fabrício Klerynton
Reis, Rodrigo A.
Huergo, Luciano F.
dc.subject.other.pt_BR.fl_str_mv SARS-CoV-2
COVID-19
ELISA
Proteínas do Nucleocapsídeo
topic SARS-CoV-2
COVID-19
ELISA
Proteínas do Nucleocapsídeo
Immunologic Tests
Enzyme-Linked Immunosorbent Assay
Nucleocapsid Proteins
Pruebas Inmunológicas
Ensayo de Inmunoadsorción Enzimática
Proteínas de la Nucleocápside
Tests immunologiques
Test ELISA
Protéines nucléocapside
Testes Imunológicos
Ensaio de Imunoadsorção Enzimática
dc.subject.en.pt_BR.fl_str_mv Immunologic Tests
Enzyme-Linked Immunosorbent Assay
Nucleocapsid Proteins
dc.subject.es.pt_BR.fl_str_mv Pruebas Inmunológicas
Ensayo de Inmunoadsorción Enzimática
Proteínas de la Nucleocápside
dc.subject.fr.pt_BR.fl_str_mv Tests immunologiques
Test ELISA
Protéines nucléocapside
dc.subject.decs.pt_BR.fl_str_mv Testes Imunológicos
Ensaio de Imunoadsorção Enzimática
description Universidade Federal do Paraná. Setor Litoral. Matinhos, PR, Brasil.
publishDate 2021
dc.date.accessioned.fl_str_mv 2021-11-12T15:24:25Z
dc.date.available.fl_str_mv 2021-11-12T15:24:25Z
dc.date.issued.fl_str_mv 2021
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CONZENTINO, Marcelo S. et al. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion. Brazilian Journal of Microbiology. p. 1–5, 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/49817
dc.identifier.issn.pt_BR.fl_str_mv 1678-4405
dc.identifier.doi.none.fl_str_mv 10.1007/s42770-021-00556-6
identifier_str_mv CONZENTINO, Marcelo S. et al. Antigen production and development of an indirect ELISA based on the nucleocapsid protein to detect human SARS‑CoV‑2 seroconversion. Brazilian Journal of Microbiology. p. 1–5, 2021.
1678-4405
10.1007/s42770-021-00556-6
url https://www.arca.fiocruz.br/handle/icict/49817
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/49817/1/license.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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