Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)

Detalhes bibliográficos
Autor(a) principal: Ferraz, Rosana Paula Cruz
Data de Publicação: 2014
Outros Autores: Bomfim, Diogo S, Carvalho, Nanashara Coelho de, Soares, Milena Botelho Pereira, Pinheiro, Maria Lúcia Bélem, Costa, Emmanoel Vilaça, Bezerra, Daniel Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7856
Resumo: Universidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, Brasil
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spelling Ferraz, Rosana Paula CruzBomfim, Diogo SCarvalho, Nanashara Coelho deSoares, Milena Botelho PereiraPinheiro, Maria Lúcia BélemCosta, Emmanoel VilaçaBezerra, Daniel Pereira2014-06-30T17:07:11Z2014-06-30T17:07:11Z2014FERRAZ, r. p. c. et al. Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae). Flavour and Fragrance Journal, v. 29, n. 4, p.228-232, 2014.https://www.arca.fiocruz.br/handle/icict/785610.1002/ffj.3199engJohn Wiley & Sons, LtdCytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, BrasilUniversidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Hospital São Rafael. Centro de Biotecnologia e Terapia Celular. Salvador, BA, BrasilUniversidade Federal do Amazonas. Departamento de Química. Manaus, AM, BrasilUniversidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilGuatteria blepharophylla Mart. (synonym Guatteriopsis blepharophylla Mart.) and Guatteria hispida (R.E. Fr.) Erkens & Maas (synonym Guatteriopsis hispida R.E. Fr.) belong to the Annonaceae family and are found in the Brazilian and Colombian Amazon basin. Both species are popularly known as ‘envira’ or ‘envireira’. In the present study, the leaf essential oils of G. blepharophylla (EOGB) and G. hispida (EOGH) were selected to investigate their cytotoxic effects. Tumour cell lines were treated with increasing concentrations of both essential oils for 72 h and analysed by a methyl-[3H]thymidine incorporation assay. The pro-apoptotic effect of these essential oils was assessed in HepG2 cells by morphological analysis (using haematoxylin/eosin staining and acridine orange/ethidium bromide staining), flow cytometry (cell membrane integrity and internucleosomal DNA fragmentation analysis) and a caspase-3 activation assay after 24 h incubation. Both essential oils displayed potent cytotoxicity in different tumour cell lines. EOGB showed IC50 values from 6.03 to 16.46 ìg/ml for HepG2 and K562 cell lines, and EOGH showed IC50 values from 5.45 to 24.89 ìg/ml for HepG2 and K562 cell lines, respectively. Cell morphologies consistent with apoptosis and a remarkable activation of caspase-3 were observed in the HepG2 cells treated with essential oils for 24 h. Significant increases in internucleosomal DNA fragmentation without altered membrane integrity were also found. In conclusion, both essential oils investigated were able to inhibit tumour cell proliferation and induce cell death by apoptosis pathways.Guatteria blepharophyllaGuatteria hispidaAnnonaceaeCytotoxicityApoptosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFerraz RPC Cytotoxic properties....pdfFerraz RPC Cytotoxic properties....pdfapplication/pdf435315https://www.arca.fiocruz.br/bitstream/icict/7856/1/Ferraz%20RPC%20Cytotoxic%20properties....pdf3cd602b19468b91cba022c82409f37d0MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7856/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTFerraz RPC Cytotoxic properties....pdf.txtFerraz RPC Cytotoxic properties....pdf.txtExtracted texttext/plain23276https://www.arca.fiocruz.br/bitstream/icict/7856/3/Ferraz%20RPC%20Cytotoxic%20properties....pdf.txtb295ced0092b8a98c44ef3d500da4124MD53icict/78562023-03-15 14:33:16.71oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:16Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
title Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
spellingShingle Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
Ferraz, Rosana Paula Cruz
Guatteria blepharophylla
Guatteria hispida
Annonaceae
Cytotoxicity
Apoptosis
title_short Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
title_full Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
title_fullStr Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
title_full_unstemmed Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
title_sort Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae)
author Ferraz, Rosana Paula Cruz
author_facet Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author_role author
author2 Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ferraz, Rosana Paula Cruz
Bomfim, Diogo S
Carvalho, Nanashara Coelho de
Soares, Milena Botelho Pereira
Pinheiro, Maria Lúcia Bélem
Costa, Emmanoel Vilaça
Bezerra, Daniel Pereira
dc.subject.en.pt_BR.fl_str_mv Guatteria blepharophylla
Guatteria hispida
Annonaceae
Cytotoxicity
Apoptosis
topic Guatteria blepharophylla
Guatteria hispida
Annonaceae
Cytotoxicity
Apoptosis
description Universidade Federal de Sergipe. Departamento de Fisiologia. São Cristóvão, SE, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-06-30T17:07:11Z
dc.date.available.fl_str_mv 2014-06-30T17:07:11Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERRAZ, r. p. c. et al. Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae). Flavour and Fragrance Journal, v. 29, n. 4, p.228-232, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7856
dc.identifier.doi.none.fl_str_mv 10.1002/ffj.3199
identifier_str_mv FERRAZ, r. p. c. et al. Cytotoxic properties of the leaf essential oils of Guatteria blepharophylla and Guatteria hispida (Annonaceae). Flavour and Fragrance Journal, v. 29, n. 4, p.228-232, 2014.
10.1002/ffj.3199
url https://www.arca.fiocruz.br/handle/icict/7856
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv John Wiley & Sons, Ltd
publisher.none.fl_str_mv John Wiley & Sons, Ltd
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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