Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus

Detalhes bibliográficos
Autor(a) principal: Ferreira, Nelson R.
Data de Publicação: 2010
Outros Autores: Sarquis, Maria Inez M., Alves, Cláudio N., Santos, Alberdan S.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25727
Resumo: The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ -pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28◦C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l−1 in 120g l−1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP.
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spelling Ferreira, Nelson R.Sarquis, Maria Inez M.Alves, Cláudio N.Santos, Alberdan S.2018-04-10T14:57:11Z2018-04-10T14:57:11Z2010FERREIRA, Nelson R. et al. Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus. Anais da Academia Brasileira de Ciências, v.82, n.3, p.569-576, 2010.0001-3765https://www.arca.fiocruz.br/handle/icict/257271678-2690The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ -pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28◦C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l−1 in 120g l−1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP.Universidade Federal do Pará. Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos. Belém, PA, Brasil / Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Coleção de Cultura de Fungos. Rio de Janeiro, RJ. Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. Belém, PA, Brasil.Universidade Federal do Pará. Programa de Pós-Graduação em Ciência e Tecnologia de Alimentos. Belém, PA, Brasil / Universidade Federal do Pará. Programa de Pós-Graduação em Química. Laboratório de Investigação Sistemática em Biotecnologia e Química Fina. 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dc.title.pt_BR.fl_str_mv Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
title Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
spellingShingle Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
Ferreira, Nelson R.
biotransformação
ácido kójico
Aspergillus flavus
metabolito secundário
Aspergillus flavus
biotransformation
kojic acid
secondary metabolite
fructose-isomerase
title_short Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
title_full Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
title_fullStr Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
title_full_unstemmed Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
title_sort Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus
author Ferreira, Nelson R.
author_facet Ferreira, Nelson R.
Sarquis, Maria Inez M.
Alves, Cláudio N.
Santos, Alberdan S.
author_role author
author2 Sarquis, Maria Inez M.
Alves, Cláudio N.
Santos, Alberdan S.
author2_role author
author
author
dc.contributor.author.fl_str_mv Ferreira, Nelson R.
Sarquis, Maria Inez M.
Alves, Cláudio N.
Santos, Alberdan S.
dc.subject.other.pt_BR.fl_str_mv biotransformação
ácido kójico
Aspergillus flavus
metabolito secundário
topic biotransformação
ácido kójico
Aspergillus flavus
metabolito secundário
Aspergillus flavus
biotransformation
kojic acid
secondary metabolite
fructose-isomerase
dc.subject.en.pt_BR.fl_str_mv Aspergillus flavus
biotransformation
kojic acid
secondary metabolite
fructose-isomerase
description The sucrose hydrolysis and the preference of consumption of glucose instead of fructose were investigated for the production of 5-hydroxy-2-hydroxymethyl-γ -pyrone (HHMP) in the presence of Aspergillus flavus IOC 3974 cultivated in liquid Czapeck medium. Standardized 0.5g of pellets were transferred as inoculum into twelve conical flasks of 250 ml containing 100 ml of medium with different sucrose concentration, which was kept at 120 rpm and 28◦C for 16 days without pH adjustment. Aliquots of 500μl of the broth culture were withdrawn at 24 h intervals and analyzed. The major yield of HHMP was 26g l−1 in 120g l−1 of sucrose. At these conditions, A. flavus produced an invertase capable of hydrolyzing 65% of total sucrose concentration in 24h, and an isomerase capable of converting fructose into glucose. In this work, it focused the preference for glucose and, then, of fructose by A. flavus and the strategy used to produce HHMP.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-04-10T14:57:11Z
dc.date.available.fl_str_mv 2018-04-10T14:57:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, Nelson R. et al. Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus. Anais da Academia Brasileira de Ciências, v.82, n.3, p.569-576, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25727
dc.identifier.issn.pt_BR.fl_str_mv 0001-3765
dc.identifier.eissn.none.fl_str_mv 1678-2690
identifier_str_mv FERREIRA, Nelson R. et al. Biotransformation of sucrose into 5-hydroxy-2-hydroxymethyl-γ -pirone by Aspergillus flavus. Anais da Academia Brasileira de Ciências, v.82, n.3, p.569-576, 2010.
0001-3765
1678-2690
url https://www.arca.fiocruz.br/handle/icict/25727
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Academia Brasileira de Ciências
publisher.none.fl_str_mv Academia Brasileira de Ciências
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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