Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva

Detalhes bibliográficos
Autor(a) principal: Cunha, Jurema de Melo
Data de Publicação: 2018
Outros Autores: Abbehusen, Melissa Moura Costa, Suarez, Martha, Valenzuela, Jesus, Teixeira, Clarissa Romero, Brodskyn, Claudia Ida
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25842
Resumo: CNPq.
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spelling Cunha, Jurema de MeloAbbehusen, Melissa Moura CostaSuarez, MarthaValenzuela, JesusTeixeira, Clarissa RomeroBrodskyn, Claudia Ida2018-04-13T19:08:39Z2018-04-13T19:08:39Z2018CUNHA, J. M. et al. Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/2584210.1016/j.actatropica.2017.10.009CNPq.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Fiocruz Piauí. Teresina, PI, Brasil.National Institutes of Health. National Institute of Allergy and Infectious Diseases. Laboratory of Malaria and Vector Research. Vector Molecular Biology Section. Rockville, MD, USAFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Instituto Nacional de Ciência e Tecnologia de Investigação em Imunologia. Salvador, BA, BrasilLeishmania is transmitted in the presence of sand fly saliva. Protective immunity generated by saliva has encouraged identification of a vector salivary-based vaccine. Previous studies have shown that immunization with LJM11, a salivary protein from Lutzomyia longipalpis, is able to induce a Th1 immune response and protect mice against bites of Leishmania major-infected Lutzomyia longipalpis. Here, we further investigate if immunization with LJM11 recombinant protein is able to confer cross-protection against infection with Leishmania braziliensis associated with salivary gland sonicate (SGS) from Lutzomyia intermedia or Lu. longipalpis. Mice immunized with LJM11 protein exhibited an increased production of anti-LJM11 IgG, IgG1 and IgG2a and a DTH response characterized by an inflammatory infiltrate with the presence of CD4+ IFN-γ+ T cells. LJM11-immunized mice were intradermally infected in the ear with L. braziliensis in the presence of Lu. longipalpis or Lu. intermedia SGS. A significant reduction of parasite numbers in the ear and lymph node in the group challenged with L. braziliensis plus Lu. longipalpis SGS was observed, but not when the challenge was performed with L. braziliensis plus Lu. intermedia SGS. A higher specific production of IFN-γ and absence of IL-10 by lymph node cells were only observed in LJM11 immunized mice after infection. After two weeks, a similar frequency of CD4+ IFN-γ+ T cells was detected in LJM11 and BSA groups challenged with L. braziliensis plus Lu. longipalpis SGS, suggesting that early events possibly triggered by immunization are essential for protection against Leishmania infection. Our findings support the specificity of saliva-mediated immune responses and reinforce the importance of identifying cross-protective salivary antigens.engElsevierLeishmania braziliensisLutzomyia intermediaLutzomyia longipalpisVacinaLeishmania braziliensisSGSLJM11Lutzomyia intermediaLutzomyia longipalpisVaccineImmunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis salivainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25842/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCunha J Immunization with LJM11 ....pdfCunha J Immunization with LJM11 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dc.title.pt_BR.fl_str_mv Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
title Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
spellingShingle Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
Cunha, Jurema de Melo
Leishmania braziliensis
Lutzomyia intermedia
Lutzomyia longipalpis
Vacina
Leishmania braziliensis
SGS
LJM11
Lutzomyia intermedia
Lutzomyia longipalpis
Vaccine
title_short Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
title_full Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
title_fullStr Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
title_full_unstemmed Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
title_sort Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva
author Cunha, Jurema de Melo
author_facet Cunha, Jurema de Melo
Abbehusen, Melissa Moura Costa
Suarez, Martha
Valenzuela, Jesus
Teixeira, Clarissa Romero
Brodskyn, Claudia Ida
author_role author
author2 Abbehusen, Melissa Moura Costa
Suarez, Martha
Valenzuela, Jesus
Teixeira, Clarissa Romero
Brodskyn, Claudia Ida
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Jurema de Melo
Abbehusen, Melissa Moura Costa
Suarez, Martha
Valenzuela, Jesus
Teixeira, Clarissa Romero
Brodskyn, Claudia Ida
dc.subject.other.pt_BR.fl_str_mv Leishmania braziliensis
Lutzomyia intermedia
Lutzomyia longipalpis
Vacina
topic Leishmania braziliensis
Lutzomyia intermedia
Lutzomyia longipalpis
Vacina
Leishmania braziliensis
SGS
LJM11
Lutzomyia intermedia
Lutzomyia longipalpis
Vaccine
dc.subject.en.pt_BR.fl_str_mv Leishmania braziliensis
SGS
LJM11
Lutzomyia intermedia
Lutzomyia longipalpis
Vaccine
description CNPq.
publishDate 2018
dc.date.accessioned.fl_str_mv 2018-04-13T19:08:39Z
dc.date.available.fl_str_mv 2018-04-13T19:08:39Z
dc.date.issued.fl_str_mv 2018
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CUNHA, J. M. et al. Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25842
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2017.10.009
identifier_str_mv CUNHA, J. M. et al. Immunization with LJM11 salivary protein protects against infection with Leishmania braziliensis in the presence of Lutzomyia longipalpis saliva.
0001-706X
10.1016/j.actatropica.2017.10.009
url https://www.arca.fiocruz.br/handle/icict/25842
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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