Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria

Detalhes bibliográficos
Autor(a) principal: Eugenio, Mateus
Data de Publicação: 2016
Outros Autores: Müller, Nathalia, Frasés, Susana, Almeida-Paes, Rodrigo, Lima, Luís Maurício T. R., Lemgruber, Leandro, Farina, Marcos, Souza, Wanderley de, Sant’Anna, Celso
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32715
Resumo: National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
id CRUZ_24e225710dff69db9186d6fb53f42344
oai_identifier_str oai:www.arca.fiocruz.br:icict/32715
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Eugenio, MateusMüller, NathaliaFrasés, SusanaAlmeida-Paes, RodrigoLima, Luís Maurício T. R.Lemgruber, LeandroFarina, MarcosSouza, Wanderley deSant’Anna, Celso2019-04-24T14:18:17Z2019-04-24T14:18:17Z2016EUGENIO, Mateus et al. Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Advances, p. 1-38, 2016.2046-2069https://www.arca.fiocruz.br/handle/icict/3271510.1039/C5RA22727E2046-2069engRoyal Society of ChemistryYeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteriainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNational Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.Fundação Oswaldo Cruz. instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Laboratory of Pharmaceutical Biotechnology. Rio de Janeiro, RJ, Brazil.National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.Laboratory of Biomineralization, Federal University of Rio de Janeiro, Rio de Janeiro, RJ, BrazilNational Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil / Federal University of Rio de Janeiro. Laboratory of Cellular Ultrastructure Hertha Meyer. Rio de Janeiro, RJ, Brazil.National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil / National Institute of Science and Technology for Structural Biology and Bioimaging. Rio de Janeiro, RJ, Brazil.Here, we provided the first evidences of yeast strains assisted Ag/AgCl-NPs production in vitro. The formed nanoparticles were characterized by spectroscopic and electron microscopy approaches. UV-Vis supported the biosynthesis. TEM analysis evidenced that nanoparticles mainly presented circular shape and their diameter varied mostly in the range from 2 to 10 nm. XRD analysis showed a crystalline structure, with diffraction peaks corresponding to metallic silver and silver chloride nanoparticles, and when analyzed by high-resolution transmission electron microscopy (HRTEM), instead of being round, (111) (octahedral) and (200) (cubic-) symmetry facets appeared systematically in one side of the nanoparticles. Analysis of ultra-thin sections by TEM indicated that the domain of the synthesis of Ag/AgCl-NPs were mainly between cell wall and the plasma membrane. By using 3D reconstruction obtained from focused ion beam scanning electron microscopy (FIB/SEM) the spatial distribution of the domains of nanoparticles synthesis was mapped and nanoaggregates of Ag/AgCl-NPs up 35 nm in diameter were observed. Extracellular synthesis also occurred; in accordance with the fact that conditioned media from yeast isolates were as efficient at producing Ag/AgCl-NPs as live-cell cultures. Exposure of Gram-positive Staphylococcus aureus and Gram-negative Klebsiella pneumoniae cultures to Ag/AgCl-NPs led to a strong growth inhibition as shown by optical density measurements. The Ag/AgCl-NPs described here have characteristics compatible with a strong potential for use in the biotechnology industry, particularly for biomedical applications.BiotechnologyYeastsMetallic nanoparticlesBiomimetic synthesisSilver/silver chloride nanoparticlesSimNão publicadoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32715/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALva_Eugenio_Mateus_etal_INI_2016.pdfva_Eugenio_Mateus_etal_INI_2016.pdfapplication/pdf3340635https://www.arca.fiocruz.br/bitstream/icict/32715/2/va_Eugenio_Mateus_etal_INI_2016.pdf1def908aa54b560b8ad71596ce252f9eMD52TEXTva_Eugenio_Mateus_etal_INI_2016.pdf.txtva_Eugenio_Mateus_etal_INI_2016.pdf.txtExtracted texttext/plain55728https://www.arca.fiocruz.br/bitstream/icict/32715/3/va_Eugenio_Mateus_etal_INI_2016.pdf.txt204a5af082247098b676ec3dbd614f4aMD53icict/327152019-06-06 15:32:14.987oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-06T18:32:14Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
title Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
spellingShingle Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
Eugenio, Mateus
Biotechnology
Yeasts
Metallic nanoparticles
Biomimetic synthesis
Silver/silver chloride nanoparticles
title_short Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
title_full Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
title_fullStr Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
title_full_unstemmed Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
title_sort Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria
author Eugenio, Mateus
author_facet Eugenio, Mateus
Müller, Nathalia
Frasés, Susana
Almeida-Paes, Rodrigo
Lima, Luís Maurício T. R.
Lemgruber, Leandro
Farina, Marcos
Souza, Wanderley de
Sant’Anna, Celso
author_role author
author2 Müller, Nathalia
Frasés, Susana
Almeida-Paes, Rodrigo
Lima, Luís Maurício T. R.
Lemgruber, Leandro
Farina, Marcos
Souza, Wanderley de
Sant’Anna, Celso
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Eugenio, Mateus
Müller, Nathalia
Frasés, Susana
Almeida-Paes, Rodrigo
Lima, Luís Maurício T. R.
Lemgruber, Leandro
Farina, Marcos
Souza, Wanderley de
Sant’Anna, Celso
dc.subject.en.pt_BR.fl_str_mv Biotechnology
Yeasts
Metallic nanoparticles
Biomimetic synthesis
Silver/silver chloride nanoparticles
topic Biotechnology
Yeasts
Metallic nanoparticles
Biomimetic synthesis
Silver/silver chloride nanoparticles
description National Institute of Metrology, Quality and Technology. Directory of Metrology Applied to Life Science. Laboratory of Biotechnology. Duque de Caxias, RJ, Brazil.
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2019-04-24T14:18:17Z
dc.date.available.fl_str_mv 2019-04-24T14:18:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv EUGENIO, Mateus et al. Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Advances, p. 1-38, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32715
dc.identifier.issn.pt_BR.fl_str_mv 2046-2069
dc.identifier.doi.none.fl_str_mv 10.1039/C5RA22727E
dc.identifier.eissn.none.fl_str_mv 2046-2069
identifier_str_mv EUGENIO, Mateus et al. Yeast-derived biosynthesis of silver/silver chloride nanoparticles and their antiproliferative activity against bacteria. RSC Advances, p. 1-38, 2016.
2046-2069
10.1039/C5RA22727E
url https://www.arca.fiocruz.br/handle/icict/32715
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32715/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32715/2/va_Eugenio_Mateus_etal_INI_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/32715/3/va_Eugenio_Mateus_etal_INI_2016.pdf.txt
bitstream.checksum.fl_str_mv 79178e5f2a0eb066867a274556814938
1def908aa54b560b8ad71596ce252f9e
204a5af082247098b676ec3dbd614f4a
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324843948539904

Registros relacionados