Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples

Detalhes bibliográficos
Autor(a) principal: Macena, Lorena da Graça Pedrosa de
Data de Publicação: 2021
Outros Autores: Vieira, Carmen Baur, Maranhão, Adriana Gonçalves, Ferreira, Fernando César, Lemos, Elba Regina Sampaio, Miagostovich, Marize Pereira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/50886
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
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spelling Macena, Lorena da Graça Pedrosa deVieira, Carmen BaurMaranhão, Adriana GonçalvesFerreira, Fernando CésarLemos, Elba Regina SampaioMiagostovich, Marize Pereira2022-01-25T19:38:44Z2022-01-25T19:38:44Z2021MACENA, Lorena da Graça Pedrosa de et al. Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples. Int. J. Plant. Anim. Environ Sci, v. 11, n. 4, p. 614-626, Nov. 2021.2231-4490https://www.arca.fiocruz.br/handle/icict/5088610.26502/ijpaes.202125engFortune JournalsDiversidade genéticaHAdVJCPyVMonitoramento baseado em águas residuaisGenetic diversityHAdVJCPyVWastewater-based monitoringGenetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samplesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hantaviroses e Rickettsioses. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.Wastewater-based monitoring has been described as a non-invasive approach to assess virus distribution in a specific geographic area. In this study we assess the genetic diversity and concentration of human adenovirus (HAdV) and human polyomavirus JC (JCPyV) in wastewater samples in Rio de Janeiro, Brazil, during the Olympic Games, Rio 2016. Wastewater samples (50 mL) obtained from domestic and hospital sewages were concentrated by the skimmed milk flocculation method and processed using molecular tools. Quantitative polymerase chain reaction using the ABI PRISM 7500 Real Time TaqMan System and TaqMan Universal Master Mix II detected 18 HAdV and 17 JCPyV strains in 95% of those samples (18/19). A mean viral load of 8.6x105 genomic copies (GC)/L and 1.2x107 GC/L, was achieved for HAdV and JCPyV, respectively. Partial nucleotide sequencing using Sanger methodology revealed three HAdV species/ eight serotypes (HAdV B, D, D10, D17, D19, D22, F40 and F41) and seven genotypes/nine subtypes (JCPyV-1B, 2A, 3A, 3B, 4, 6, 7A, 8A and 8B). HAdV-D17 and -D22, as well as genotypes JCPyV-7 and -8, were detected for the first time in the country. The detection of previously undetected viruses in the region demonstrates the importance of our findings, adding data to the epidemiology of those viruses and corroborating the importance of environmental surveillance carried out from wastewater.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/50886/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLorenaGMacena_ElsRSLemos_etal_IOC_2021.pdfLorenaGMacena_ElsRSLemos_etal_IOC_2021.pdfapplication/pdf422669https://www.arca.fiocruz.br/bitstream/icict/50886/2/LorenaGMacena_ElsRSLemos_etal_IOC_2021.pdfdc92d67701c53d6352e64483eeae9e6eMD52icict/508862022-01-25 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dc.title.pt_BR.fl_str_mv Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
title Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
spellingShingle Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
Macena, Lorena da Graça Pedrosa de
Diversidade genética
HAdV
JCPyV
Monitoramento baseado em águas residuais
Genetic diversity
HAdV
JCPyV
Wastewater-based monitoring
title_short Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
title_full Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
title_fullStr Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
title_full_unstemmed Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
title_sort Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples
author Macena, Lorena da Graça Pedrosa de
author_facet Macena, Lorena da Graça Pedrosa de
Vieira, Carmen Baur
Maranhão, Adriana Gonçalves
Ferreira, Fernando César
Lemos, Elba Regina Sampaio
Miagostovich, Marize Pereira
author_role author
author2 Vieira, Carmen Baur
Maranhão, Adriana Gonçalves
Ferreira, Fernando César
Lemos, Elba Regina Sampaio
Miagostovich, Marize Pereira
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Macena, Lorena da Graça Pedrosa de
Vieira, Carmen Baur
Maranhão, Adriana Gonçalves
Ferreira, Fernando César
Lemos, Elba Regina Sampaio
Miagostovich, Marize Pereira
dc.subject.other.pt_BR.fl_str_mv Diversidade genética
HAdV
JCPyV
Monitoramento baseado em águas residuais
topic Diversidade genética
HAdV
JCPyV
Monitoramento baseado em águas residuais
Genetic diversity
HAdV
JCPyV
Wastewater-based monitoring
dc.subject.en.pt_BR.fl_str_mv Genetic diversity
HAdV
JCPyV
Wastewater-based monitoring
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.
publishDate 2021
dc.date.issued.fl_str_mv 2021
dc.date.accessioned.fl_str_mv 2022-01-25T19:38:44Z
dc.date.available.fl_str_mv 2022-01-25T19:38:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MACENA, Lorena da Graça Pedrosa de et al. Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples. Int. J. Plant. Anim. Environ Sci, v. 11, n. 4, p. 614-626, Nov. 2021.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/50886
dc.identifier.issn.pt_BR.fl_str_mv 2231-4490
dc.identifier.doi.none.fl_str_mv 10.26502/ijpaes.202125
identifier_str_mv MACENA, Lorena da Graça Pedrosa de et al. Genetic Diversity and Quantification of Human Adenoviruses and JC Polyomaviruses in Wastewater Samples. Int. J. Plant. Anim. Environ Sci, v. 11, n. 4, p. 614-626, Nov. 2021.
2231-4490
10.26502/ijpaes.202125
url https://www.arca.fiocruz.br/handle/icict/50886
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fortune Journals
publisher.none.fl_str_mv Fortune Journals
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/50886/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/50886/2/LorenaGMacena_ElsRSLemos_etal_IOC_2021.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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