Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration

Detalhes bibliográficos
Autor(a) principal: Fumian, Tulio M.
Data de Publicação: 2010
Outros Autores: Leite, José Paulo G., Castello, Alejandro A., Gaggero, Aldo, Caillou, Maria Susana L. de, Miagostovich, Marize P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25422
Resumo: 2013-01-01
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spelling Fumian, Tulio M.Leite, José Paulo G.Castello, Alejandro A.Gaggero, AldoCaillou, Maria Susana L. deMiagostovich, Marize P.2018-03-20T14:51:33Z2018-03-20T14:51:33Z2010FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010.0166-0934https://www.arca.fiocruz.br/handle/icict/2542210.1016/j.jviromet.2010.08.0171879-0984engElsevierRotavirus ALevivirusUltracentrifugaçãoMétodo de adsorção-eluiçãoReação em Cadeia da Polimerase MultiplexRotavirus APP7Multiplex qPCRUltracentrifugationAdsorption-elution methodDetection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentrationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2013-01-01Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Universidad Nacional de Quilmes Bernal. Laboratorio de Inmunología y Virología. Argentina.Universidad de Chile. Facultad de Medicina. ICBM. Programa de Virologia. Chile.Universidad de Tucumán. Instituto de Microbiología. Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Group A rotaviruses (RV-A) are the most common agents of viral gastroenteritis in children worldwide. The goal of this study was to compare two different methods to concentrate RV-A from sewage samples and to improve the detection and quantification of RV-A using a multiplex quantitative PCR assay with an internal control. Both RV-A and the internal control virus, bacteriophage PP7, were seeded into wastewater and then concentrated using either an ultrafiltration-based adsorption-elution protocol or an ultracentrifugation-based protocol. Real time multiplex quantitative PCR was used to quantify the purified RV-A and PP7, and the results of the multiplex assay were compared with the results of the monoplex assays. The ultracentrifugation-based method had a mean recovery rate of 47% (range: 34-60%), while the ultrafiltration-based adsorption-elution method had a mean recovery rate of 3.5% (range: 1.5-5.5%). These results demonstrate that ultracentrifugation is a more appropriate method for recovering RV-A from wastewater. This method together with the multiplex qPCR assay may be suitable for routine laboratory use.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25422/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarize_miagostovih_etal_IOC_2010.pdfmarize_miagostovih_etal_IOC_2010.pdfEmbargo vencidoapplication/pdf223831https://www.arca.fiocruz.br/bitstream/icict/25422/2/marize_miagostovih_etal_IOC_2010.pdf398882f435dee9ba2f5fa63c07eaaa6cMD52TEXTmarize_miagostovih_etal_IOC_2010.pdf.txtmarize_miagostovih_etal_IOC_2010.pdf.txtExtracted texttext/plain31190https://www.arca.fiocruz.br/bitstream/icict/25422/3/marize_miagostovih_etal_IOC_2010.pdf.txt81229a9105612286934bf200b74782baMD53icict/254222021-12-14 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dc.title.pt_BR.fl_str_mv Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
title Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
spellingShingle Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
Fumian, Tulio M.
Rotavirus A
Levivirus
Ultracentrifugação
Método de adsorção-eluição
Reação em Cadeia da Polimerase Multiplex
Rotavirus A
PP7
Multiplex qPCR
Ultracentrifugation
Adsorption-elution method
title_short Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
title_full Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
title_fullStr Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
title_full_unstemmed Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
title_sort Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
author Fumian, Tulio M.
author_facet Fumian, Tulio M.
Leite, José Paulo G.
Castello, Alejandro A.
Gaggero, Aldo
Caillou, Maria Susana L. de
Miagostovich, Marize P.
author_role author
author2 Leite, José Paulo G.
Castello, Alejandro A.
Gaggero, Aldo
Caillou, Maria Susana L. de
Miagostovich, Marize P.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fumian, Tulio M.
Leite, José Paulo G.
Castello, Alejandro A.
Gaggero, Aldo
Caillou, Maria Susana L. de
Miagostovich, Marize P.
dc.subject.other.pt_BR.fl_str_mv Rotavirus A
Levivirus
Ultracentrifugação
Método de adsorção-eluição
Reação em Cadeia da Polimerase Multiplex
topic Rotavirus A
Levivirus
Ultracentrifugação
Método de adsorção-eluição
Reação em Cadeia da Polimerase Multiplex
Rotavirus A
PP7
Multiplex qPCR
Ultracentrifugation
Adsorption-elution method
dc.subject.en.pt_BR.fl_str_mv Rotavirus A
PP7
Multiplex qPCR
Ultracentrifugation
Adsorption-elution method
description 2013-01-01
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-03-20T14:51:33Z
dc.date.available.fl_str_mv 2018-03-20T14:51:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25422
dc.identifier.issn.pt_BR.fl_str_mv 0166-0934
dc.identifier.doi.none.fl_str_mv 10.1016/j.jviromet.2010.08.017
dc.identifier.eissn.none.fl_str_mv 1879-0984
identifier_str_mv FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010.
0166-0934
10.1016/j.jviromet.2010.08.017
1879-0984
url https://www.arca.fiocruz.br/handle/icict/25422
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/25422/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/25422/2/marize_miagostovih_etal_IOC_2010.pdf
https://www.arca.fiocruz.br/bitstream/icict/25422/3/marize_miagostovih_etal_IOC_2010.pdf.txt
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