Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25422 |
Resumo: | 2013-01-01 |
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Fumian, Tulio M.Leite, José Paulo G.Castello, Alejandro A.Gaggero, AldoCaillou, Maria Susana L. deMiagostovich, Marize P.2018-03-20T14:51:33Z2018-03-20T14:51:33Z2010FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010.0166-0934https://www.arca.fiocruz.br/handle/icict/2542210.1016/j.jviromet.2010.08.0171879-0984engElsevierRotavirus ALevivirusUltracentrifugaçãoMétodo de adsorção-eluiçãoReação em Cadeia da Polimerase MultiplexRotavirus APP7Multiplex qPCRUltracentrifugationAdsorption-elution methodDetection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentrationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2013-01-01Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Universidad Nacional de Quilmes Bernal. Laboratorio de Inmunología y Virología. Argentina.Universidad de Chile. Facultad de Medicina. ICBM. Programa de Virologia. Chile.Universidad de Tucumán. Instituto de Microbiología. Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ. Brasil.Group A rotaviruses (RV-A) are the most common agents of viral gastroenteritis in children worldwide. The goal of this study was to compare two different methods to concentrate RV-A from sewage samples and to improve the detection and quantification of RV-A using a multiplex quantitative PCR assay with an internal control. Both RV-A and the internal control virus, bacteriophage PP7, were seeded into wastewater and then concentrated using either an ultrafiltration-based adsorption-elution protocol or an ultracentrifugation-based protocol. Real time multiplex quantitative PCR was used to quantify the purified RV-A and PP7, and the results of the multiplex assay were compared with the results of the monoplex assays. The ultracentrifugation-based method had a mean recovery rate of 47% (range: 34-60%), while the ultrafiltration-based adsorption-elution method had a mean recovery rate of 3.5% (range: 1.5-5.5%). These results demonstrate that ultracentrifugation is a more appropriate method for recovering RV-A from wastewater. This method together with the multiplex qPCR assay may be suitable for routine laboratory use.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25422/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmarize_miagostovih_etal_IOC_2010.pdfmarize_miagostovih_etal_IOC_2010.pdfEmbargo vencidoapplication/pdf223831https://www.arca.fiocruz.br/bitstream/icict/25422/2/marize_miagostovih_etal_IOC_2010.pdf398882f435dee9ba2f5fa63c07eaaa6cMD52TEXTmarize_miagostovih_etal_IOC_2010.pdf.txtmarize_miagostovih_etal_IOC_2010.pdf.txtExtracted texttext/plain31190https://www.arca.fiocruz.br/bitstream/icict/25422/3/marize_miagostovih_etal_IOC_2010.pdf.txt81229a9105612286934bf200b74782baMD53icict/254222021-12-14 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dc.title.pt_BR.fl_str_mv |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
title |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
spellingShingle |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration Fumian, Tulio M. Rotavirus A Levivirus Ultracentrifugação Método de adsorção-eluição Reação em Cadeia da Polimerase Multiplex Rotavirus A PP7 Multiplex qPCR Ultracentrifugation Adsorption-elution method |
title_short |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
title_full |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
title_fullStr |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
title_full_unstemmed |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
title_sort |
Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration |
author |
Fumian, Tulio M. |
author_facet |
Fumian, Tulio M. Leite, José Paulo G. Castello, Alejandro A. Gaggero, Aldo Caillou, Maria Susana L. de Miagostovich, Marize P. |
author_role |
author |
author2 |
Leite, José Paulo G. Castello, Alejandro A. Gaggero, Aldo Caillou, Maria Susana L. de Miagostovich, Marize P. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Fumian, Tulio M. Leite, José Paulo G. Castello, Alejandro A. Gaggero, Aldo Caillou, Maria Susana L. de Miagostovich, Marize P. |
dc.subject.other.pt_BR.fl_str_mv |
Rotavirus A Levivirus Ultracentrifugação Método de adsorção-eluição Reação em Cadeia da Polimerase Multiplex |
topic |
Rotavirus A Levivirus Ultracentrifugação Método de adsorção-eluição Reação em Cadeia da Polimerase Multiplex Rotavirus A PP7 Multiplex qPCR Ultracentrifugation Adsorption-elution method |
dc.subject.en.pt_BR.fl_str_mv |
Rotavirus A PP7 Multiplex qPCR Ultracentrifugation Adsorption-elution method |
description |
2013-01-01 |
publishDate |
2010 |
dc.date.issued.fl_str_mv |
2010 |
dc.date.accessioned.fl_str_mv |
2018-03-20T14:51:33Z |
dc.date.available.fl_str_mv |
2018-03-20T14:51:33Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/25422 |
dc.identifier.issn.pt_BR.fl_str_mv |
0166-0934 |
dc.identifier.doi.none.fl_str_mv |
10.1016/j.jviromet.2010.08.017 |
dc.identifier.eissn.none.fl_str_mv |
1879-0984 |
identifier_str_mv |
FUMIAN, Tulio M. et al. Detection of rotavirus A in sewage samples using multiplex qPCR and an evaluation of the ultracentrifugation and adsorption-elution methods for virus concentration. Journal of Virological Methods, v. 170, p. 42–46, 2010. 0166-0934 10.1016/j.jviromet.2010.08.017 1879-0984 |
url |
https://www.arca.fiocruz.br/handle/icict/25422 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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Fundação Oswaldo Cruz (FIOCRUZ) |
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FIOCRUZ |
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FIOCRUZ |
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Repositório Institucional da FIOCRUZ (ARCA) |
collection |
Repositório Institucional da FIOCRUZ (ARCA) |
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