Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening

Detalhes bibliográficos
Autor(a) principal: Chalegre, Karlos Diogo de Melo
Data de Publicação: 2009
Outros Autores: Romão, Tatiany Patrícia, Amorim, Liliane Barbosa, Anastacio, Daniela Bandeira, Barros, Rosineide Arruda de, Oliveira, Cláudia Maria Fontes de, Regis, Lêda, Melo Neto, Osvaldo Pompílio de, Silva Filha, Maria Helena Neves Lobo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27501
Resumo: Este projeto foi financiado por doações do Conselho Nacional de Pesquisa (CNPq Brasil, 471911 / 2006-2) e da Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, APQ 0060-2.02 / 07).
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spelling Chalegre, Karlos Diogo de MeloRomão, Tatiany PatríciaAmorim, Liliane BarbosaAnastacio, Daniela BandeiraBarros, Rosineide Arruda deOliveira, Cláudia Maria Fontes deRegis, LêdaMelo Neto, Osvaldo Pompílio deSilva Filha, Maria Helena Neves Lobo2018-07-12T13:13:31Z2018-07-12T13:13:31Z2009CHALEGRE, K. D. DE M. et al. Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening. Applied and Environmental Microbiology, v. 75, n. 4, p. 1044–1049, fev. 2009.1098-5336https://www.arca.fiocruz.br/handle/icict/2750110.1128/AEM.02032-08Este projeto foi financiado por doações do Conselho Nacional de Pesquisa (CNPq Brasil, 471911 / 2006-2) e da Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, APQ 0060-2.02 / 07).Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Microbiologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de Entomologia. Recife, PE, Brasil.The activity of the Bacillus sphaericus binary (Bin) toxin on Culex quinquefasciatus larvae depends on its specific binding to the Cqm1 receptor, a midgut membrane-bound alpha-glucosidase. A 19-nucleotide deletion in the cqm1 gene (cqm1(REC)) mediates high-level resistance to Bin toxin. Here, resistance in nontreated and B. sphaericus-treated field populations of C. quinquefasciatus was assessed through bioassays as well as a specific PCR assay designed to detect the cqm1(REC) allele in individual larvae. Resistance ratios at 90% lethal concentration, gathered through bioassays, were close to 1 and indicate that the selected populations had similar levels of susceptibility to B. sphaericus, comparable to that of a laboratory colony. A diagnostic PCR assay detected the cqm1(REC) allele in all populations investigated, and its frequency in two nontreated areas was 0.006 and 0.003, while the frequency in the B. sphaericus-treated population was significantly higher. Values of 0.053 and 0.055 were detected for two distinct sets of samples, and homozygote resistant larvae were found. Evaluation of Cqm1 expression in individual larvae through alpha-glucosidase assays corroborated the allelic frequency revealed by PCR. The data from this study indicate that the cqm1(REC) allele was present at a detectable frequency in nontreated populations, while the higher frequency in samples from the treated area is, perhaps, correlated with the exposure to B. sphaericus. This is the first report of the molecular detection of a biolarvicide resistance allele in mosquito populations, and it confirms that the PCR-based approach is suitable to track such alleles in target populations.engCulex quinquefasciatusPCRAlelosCulex quinquefasciatusPCRAllelesAlelosAnimaisToxinas bacterianas / toxicidadeCulex / efeitos de drogasCulex / genéticaResistência a drogaFreqüência GenéticaGenes, InsetoHomozigotoImunidade InataProteínas de Insetos / genéticaProteínas de Insetos / metabolismoReação em Cadeia da Polimerase / métodosSupressão de Seqüênciaalfa-glucosidases / genéticaalfa-glucosidases / metabolismoDetection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screeninginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/27501/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALDetection of an Allele Conferring Resistance to Bacillus sphaericus Binary.pdfDetection of an Allele Conferring Resistance to Bacillus sphaericus Binary.pdfapplication/pdf217769https://www.arca.fiocruz.br/bitstream/icict/27501/2/Detection%20of%20an%20Allele%20Conferring%20Resistance%20to%20Bacillus%20sphaericus%20Binary.pdfd47e225369d391dfbc4a798ef51c414eMD52TEXTDetection of an Allele Conferring Resistance to Bacillus sphaericus Binary.pdf.txtDetection of an Allele Conferring Resistance to Bacillus sphaericus Binary.pdf.txtExtracted texttext/plain37969https://www.arca.fiocruz.br/bitstream/icict/27501/3/Detection%20of%20an%20Allele%20Conferring%20Resistance%20to%20Bacillus%20sphaericus%20Binary.pdf.txt2ccbd9dc6b72a27b7c79cb9313f1d0c8MD53icict/275012018-08-15 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dc.title.pt_BR.fl_str_mv Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
title Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
spellingShingle Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
Chalegre, Karlos Diogo de Melo
Culex quinquefasciatus
PCR
Alelos
Culex quinquefasciatus
PCR
Alleles
Alelos
Animais
Toxinas bacterianas / toxicidade
Culex / efeitos de drogas
Culex / genética
Resistência a droga
Freqüência Genética
Genes, Inseto
Homozigoto
Imunidade Inata
Proteínas de Insetos / genética
Proteínas de Insetos / metabolismo
Reação em Cadeia da Polimerase / métodos
Supressão de Seqüência
alfa-glucosidases / genética
alfa-glucosidases / metabolismo
title_short Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
title_full Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
title_fullStr Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
title_full_unstemmed Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
title_sort Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening
author Chalegre, Karlos Diogo de Melo
author_facet Chalegre, Karlos Diogo de Melo
Romão, Tatiany Patrícia
Amorim, Liliane Barbosa
Anastacio, Daniela Bandeira
Barros, Rosineide Arruda de
Oliveira, Cláudia Maria Fontes de
Regis, Lêda
Melo Neto, Osvaldo Pompílio de
Silva Filha, Maria Helena Neves Lobo
author_role author
author2 Romão, Tatiany Patrícia
Amorim, Liliane Barbosa
Anastacio, Daniela Bandeira
Barros, Rosineide Arruda de
Oliveira, Cláudia Maria Fontes de
Regis, Lêda
Melo Neto, Osvaldo Pompílio de
Silva Filha, Maria Helena Neves Lobo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Chalegre, Karlos Diogo de Melo
Romão, Tatiany Patrícia
Amorim, Liliane Barbosa
Anastacio, Daniela Bandeira
Barros, Rosineide Arruda de
Oliveira, Cláudia Maria Fontes de
Regis, Lêda
Melo Neto, Osvaldo Pompílio de
Silva Filha, Maria Helena Neves Lobo
dc.subject.other.pt_BR.fl_str_mv Culex quinquefasciatus
PCR
Alelos
topic Culex quinquefasciatus
PCR
Alelos
Culex quinquefasciatus
PCR
Alleles
Alelos
Animais
Toxinas bacterianas / toxicidade
Culex / efeitos de drogas
Culex / genética
Resistência a droga
Freqüência Genética
Genes, Inseto
Homozigoto
Imunidade Inata
Proteínas de Insetos / genética
Proteínas de Insetos / metabolismo
Reação em Cadeia da Polimerase / métodos
Supressão de Seqüência
alfa-glucosidases / genética
alfa-glucosidases / metabolismo
dc.subject.en.pt_BR.fl_str_mv Culex quinquefasciatus
PCR
Alleles
dc.subject.decs.pt_BR.fl_str_mv Alelos
Animais
Toxinas bacterianas / toxicidade
Culex / efeitos de drogas
Culex / genética
Resistência a droga
Freqüência Genética
Genes, Inseto
Homozigoto
Imunidade Inata
Proteínas de Insetos / genética
Proteínas de Insetos / metabolismo
Reação em Cadeia da Polimerase / métodos
Supressão de Seqüência
alfa-glucosidases / genética
alfa-glucosidases / metabolismo
description Este projeto foi financiado por doações do Conselho Nacional de Pesquisa (CNPq Brasil, 471911 / 2006-2) e da Fundação de Amparo à Ciência e Tecnologia do Estado de Pernambuco (FACEPE Brasil, APQ 0060-2.02 / 07).
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2018-07-12T13:13:31Z
dc.date.available.fl_str_mv 2018-07-12T13:13:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHALEGRE, K. D. DE M. et al. Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening. Applied and Environmental Microbiology, v. 75, n. 4, p. 1044–1049, fev. 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27501
dc.identifier.issn.pt_BR.fl_str_mv 1098-5336
dc.identifier.doi.none.fl_str_mv 10.1128/AEM.02032-08
identifier_str_mv CHALEGRE, K. D. DE M. et al. Detection of an allele conferring resistance to Bacillus sphaericus binary toxin in Culex quinquefasciatus populations by molecular screening. Applied and Environmental Microbiology, v. 75, n. 4, p. 1044–1049, fev. 2009.
1098-5336
10.1128/AEM.02032-08
url https://www.arca.fiocruz.br/handle/icict/27501
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27501/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/27501/2/Detection%20of%20an%20Allele%20Conferring%20Resistance%20to%20Bacillus%20sphaericus%20Binary.pdf
https://www.arca.fiocruz.br/bitstream/icict/27501/3/Detection%20of%20an%20Allele%20Conferring%20Resistance%20to%20Bacillus%20sphaericus%20Binary.pdf.txt
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