Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/59111 |
Resumo: | GenoScreen, Lille, France. |
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Jouet, AgatheBraet, Sofie MarijkeGaudin, CyrilBisch, Gaëlle BischVasconcellos, SidraLivramento, Rebecca Emmanuela Epaminondas Nicacio de Oliveira doPalacios, Yrneh Yadamis PradoFontes, Amanda BrumLucena, NormaRosa, PatriciaMoraes, MiltonLa, KevinBadalato, NellyLenoir, EstebanFerré, AliceClément, MarieHasker, EpcoGrillone, Silahi HalifaAbdou, WirdaneSaid, AouladiAssoumani, YounoussaAttoumani, NissadLaurent, YannickCambau, EmmanuelleJong, Bouke Catherine deSuffys, Philip NoëlSupply, Philip2023-06-21T18:50:08Z2023-06-21T18:50:08Z2023JOUET, Agathe et al. Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep. eBioMedicine .v.93.104649, 2023.2352-3964https://www.arca.fiocruz.br/handle/icict/5911110. 1016/j.ebiom.2023. 104649engElsevier B.V.Mycobacterium lepraeSequenciamento de próxima geração direcionadoResistência a antibióticosDuplicação de domínio genéticoDiagnósitcoVigilânciaMycobacterium lepraeTargeted next generation sequencingAntibiotic resistanceGene domain duplicationDiagnosticsSurveillanceHi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lepinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleGenoScreen, Lille, France.Institute of Tropical Medicine, Antwerp, Belgium / Department of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Antwerp, Belgium / Research Foundation Flanders, Brussels, Belgium .GenoScreen, Lille, France.GenoScreen, Lille, France.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. CPqAM/Fiocruz Recife, Pernambuco, Brasil.Fundação Oswaldo Cruz. CPqAM/Fiocruz Recife, Pernambuco, Brasil.Instituto Lauro de Souza Lima, Bauru, São Paulo, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.APHP-GHU Paris Nord Hôpital Bichat, Service de mycobactériologie spécialisée et de référence, Centre National de Référence des Mycobactéries et de la Résistance des Mycobactéries aux Antituberculeux - Laboratoire Associé, Paris, France / Université Paris Cité, INSERM, IAME UMR1137, Paris, France.GenoScreen, Lille, France.GenoScreen, Lille, France.GenoScreen, Lille, France.GenoScreen, Lille, France.Institute of Tropical Medicine, Antwerp, Belgium.Damien Foundation, Comoros.Damien Foundation, Comoros.Damien Foundation, Comoros.Damien Foundation, Comoros / National Tuberculosis and Leprosy Control Program, Moroni, Comoros.Damien Foundation, Comoros.GenoScreen, Lille, France.Université Paris Cité, INSERM, IAME UMR1137, Paris, France / Université Paris Cité, INSERM, IAME UMR1137, Paris, France.Institute of Tropical Medicine, Antwerp, Belgium.Fundação Oswaldo Cruz, Instituto Oswaldo Cruz, Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Univ. Lille, CNRS, INSERM, CHU Lille, Institut Pasteur de Lille, U1019 - UMR 8204 - CIIL - Center for Infection and Immunity of Lille, F-59000 Lille, France.Expansion of antimicrobial resistance monitoring and epidemiological surveillance are key components of the WHO strategy towards zero leprosy. The inability to grow Mycobacterium leprae in vitro precludes routine phenotypic drug susceptibility testing, and only limited molecular tests are available. We evaluated a culture-free targeted deep sequencing assay, for mycobacterial identification, genotyping based on 18 canonical SNPs and 11 core variable-number tandem-repeat (VNTR) markers, and detection of rifampicin, dapsone and fluoroquinolone resistance-associated mutations in rpoB/ctpC/ctpI, folP1, gyrA/gyrB, respectively, and hypermutation-associated mutations in nth.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/59111/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSidraVasconcelos_MiltonMoraes_etal_IOC_2023.pdfSidraVasconcelos_MiltonMoraes_etal_IOC_2023.pdfapplication/pdf1526768https://www.arca.fiocruz.br/bitstream/icict/59111/2/SidraVasconcelos_MiltonMoraes_etal_IOC_2023.pdf0b8d81dce4a16e89a775d5f5daf5a4f0MD52icict/591112023-09-14 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| dc.title.en_US.fl_str_mv |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| title |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| spellingShingle |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep Jouet, Agathe Mycobacterium leprae Sequenciamento de próxima geração direcionado Resistência a antibióticos Duplicação de domínio genético Diagnósitco Vigilância Mycobacterium leprae Targeted next generation sequencing Antibiotic resistance Gene domain duplication Diagnostics Surveillance |
| title_short |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| title_full |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| title_fullStr |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| title_full_unstemmed |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| title_sort |
Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep |
| author |
Jouet, Agathe |
| author_facet |
Jouet, Agathe Braet, Sofie Marijke Gaudin, Cyril Bisch, Gaëlle Bisch Vasconcellos, Sidra Livramento, Rebecca Emmanuela Epaminondas Nicacio de Oliveira do Palacios, Yrneh Yadamis Prado Fontes, Amanda Brum Lucena, Norma Rosa, Patricia Moraes, Milton La, Kevin Badalato, Nelly Lenoir, Esteban Ferré, Alice Clément, Marie Hasker, Epco Grillone, Silahi Halifa Abdou, Wirdane Said, Aouladi Assoumani, Younoussa Attoumani, Nissad Laurent, Yannick Cambau, Emmanuelle Jong, Bouke Catherine de Suffys, Philip Noël Supply, Philip |
| author_role |
author |
| author2 |
Braet, Sofie Marijke Gaudin, Cyril Bisch, Gaëlle Bisch Vasconcellos, Sidra Livramento, Rebecca Emmanuela Epaminondas Nicacio de Oliveira do Palacios, Yrneh Yadamis Prado Fontes, Amanda Brum Lucena, Norma Rosa, Patricia Moraes, Milton La, Kevin Badalato, Nelly Lenoir, Esteban Ferré, Alice Clément, Marie Hasker, Epco Grillone, Silahi Halifa Abdou, Wirdane Said, Aouladi Assoumani, Younoussa Attoumani, Nissad Laurent, Yannick Cambau, Emmanuelle Jong, Bouke Catherine de Suffys, Philip Noël Supply, Philip |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Jouet, Agathe Braet, Sofie Marijke Gaudin, Cyril Bisch, Gaëlle Bisch Vasconcellos, Sidra Livramento, Rebecca Emmanuela Epaminondas Nicacio de Oliveira do Palacios, Yrneh Yadamis Prado Fontes, Amanda Brum Lucena, Norma Rosa, Patricia Moraes, Milton La, Kevin Badalato, Nelly Lenoir, Esteban Ferré, Alice Clément, Marie Hasker, Epco Grillone, Silahi Halifa Abdou, Wirdane Said, Aouladi Assoumani, Younoussa Attoumani, Nissad Laurent, Yannick Cambau, Emmanuelle Jong, Bouke Catherine de Suffys, Philip Noël Supply, Philip |
| dc.subject.other.en_US.fl_str_mv |
Mycobacterium leprae Sequenciamento de próxima geração direcionado Resistência a antibióticos Duplicação de domínio genético Diagnósitco Vigilância |
| topic |
Mycobacterium leprae Sequenciamento de próxima geração direcionado Resistência a antibióticos Duplicação de domínio genético Diagnósitco Vigilância Mycobacterium leprae Targeted next generation sequencing Antibiotic resistance Gene domain duplication Diagnostics Surveillance |
| dc.subject.en.en_US.fl_str_mv |
Mycobacterium leprae Targeted next generation sequencing Antibiotic resistance Gene domain duplication Diagnostics Surveillance |
| description |
GenoScreen, Lille, France. |
| publishDate |
2023 |
| dc.date.accessioned.fl_str_mv |
2023-06-21T18:50:08Z |
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2023-06-21T18:50:08Z |
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2023 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
JOUET, Agathe et al. Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep. eBioMedicine .v.93.104649, 2023. |
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https://www.arca.fiocruz.br/handle/icict/59111 |
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2352-3964 |
| dc.identifier.doi.none.fl_str_mv |
10. 1016/j.ebiom.2023. 104649 |
| identifier_str_mv |
JOUET, Agathe et al. Hi-plex deep amplicon sequencing for identification, highresolution genotyping and multidrug resistance prediction of Mycobacterium leprae directly from patient biopsies by using Deeplex Myc-Lep. eBioMedicine .v.93.104649, 2023. 2352-3964 10. 1016/j.ebiom.2023. 104649 |
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Elsevier B.V. |
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Elsevier B.V. |
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