A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5

Detalhes bibliográficos
Autor(a) principal: Devalle, S.
Data de Publicação: 2005
Outros Autores: Niel, Christian
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/37697
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Devalle, S.Niel, Christian2019-12-07T14:07:12Z2019-12-07T14:07:12Z2005DEVALLE, S.; NIEL, C. A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylohenetic groups 1 to 5. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 6, p. 853-860, June 2005.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/3769710.1590/s0100-879x20050006000061414-431XengAssociação Brasileira de Divulgação CientíficaCoinfecçãoVírus Torque tenoReação em Cadeia da Polimerase MultiplexCo-infectionMultiplexPCRTorque teno virusA multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Torque teno virus (TTV) is a circular, single-stranded DNA virus that chronically infects healthy individuals of all ages worldwide. TTV has an extreme genetic heterogeneity which is reflected in its current classification into five main phylogenetic groups (1-5). Using specific PCR assays, it has been shown that many individuals are co-infected with TTV isolates belonging to different phylogenetic groups. Here, a multiplex PCR assay was developed, using five recombinant plasmids. Each plasmid carried an insert of different size issued from a TTV isolate belonging to a different group. The assay was able to simultaneously amplify DNAs of TTV isolates belonging to all five phylogenetic groups. Multiplex PCR was then tested satisfactorily on DNAs extracted from 55 serum samples (47 health care workers and 8 AIDS patients). All individuals but nine were infected with at least one TTV isolate. Co-infection with multiple isolates was found in 29/47 (62%) health care workers and in 8/8 (100%) AIDS patients. A number of discrepancies were observed when results obtained with three thermostable DNA polymerases were compared. For example, four TTV phylogenetic groups were detected in a particular serum sample by using one of the three DNA polymerases, whereas the other two enzymes were able to detect only three TTV groups. However, none of the three enzymes used could be broadly considered to be more efficient than the others. Despite its limitations, the assay described here constitutes a suitable tool to visualize the degree of co-infection of a given population, avoiding time-consuming experiments.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/37697/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCHristianNiel_SDevale_IOC_2005.pdfCHristianNiel_SDevale_IOC_2005.pdfapplication/pdf749210https://www.arca.fiocruz.br/bitstream/icict/37697/2/CHristianNiel_SDevale_IOC_2005.pdf98c8b50766daa86b4c3cc68e1349bf5cMD52TEXTCHristianNiel_SDevale_IOC_2005.pdf.txtCHristianNiel_SDevale_IOC_2005.pdf.txtExtracted texttext/plain26085https://www.arca.fiocruz.br/bitstream/icict/37697/3/CHristianNiel_SDevale_IOC_2005.pdf.txt278075ddb7173794aa30a03619050fc0MD53icict/376972019-12-08 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dc.title.pt_BR.fl_str_mv A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
title A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
spellingShingle A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
Devalle, S.
Coinfecção
Vírus Torque teno
Reação em Cadeia da Polimerase Multiplex
Co-infection
Multiplex
PCR
Torque teno virus
title_short A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
title_full A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
title_fullStr A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
title_full_unstemmed A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
title_sort A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylogenetic groups 1 to 5
author Devalle, S.
author_facet Devalle, S.
Niel, Christian
author_role author
author2 Niel, Christian
author2_role author
dc.contributor.author.fl_str_mv Devalle, S.
Niel, Christian
dc.subject.other.pt_BR.fl_str_mv Coinfecção
Vírus Torque teno
Reação em Cadeia da Polimerase Multiplex
topic Coinfecção
Vírus Torque teno
Reação em Cadeia da Polimerase Multiplex
Co-infection
Multiplex
PCR
Torque teno virus
dc.subject.en.pt_BR.fl_str_mv Co-infection
Multiplex
PCR
Torque teno virus
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2019-12-07T14:07:12Z
dc.date.available.fl_str_mv 2019-12-07T14:07:12Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DEVALLE, S.; NIEL, C. A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylohenetic groups 1 to 5. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 6, p. 853-860, June 2005.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/37697
dc.identifier.issn.pt_BR.fl_str_mv 0100-879X
dc.identifier.doi.none.fl_str_mv 10.1590/s0100-879x2005000600006
dc.identifier.eissn.none.fl_str_mv 1414-431X
identifier_str_mv DEVALLE, S.; NIEL, C. A multiplex PCR assay able to simultaneously detect Torque teno virus isolates from phylohenetic groups 1 to 5. Brazilian Journal of Medical and Biological Research, Ribeirão Preto, v. 38, n. 6, p. 853-860, June 2005.
0100-879X
10.1590/s0100-879x2005000600006
1414-431X
url https://www.arca.fiocruz.br/handle/icict/37697
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
publisher.none.fl_str_mv Associação Brasileira de Divulgação Científica
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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