Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/60811 |
Resumo: | Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. |
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Rizzo, JulianaTrottier, AdèleMoyrand, FrédériqueCoppée, Jean-YvesMaufrais, CorinneZimbres, Ana Claudia G.Dang, Thi Tuong ViAlanio, AlexandreDesnos-Ollivier, MarieMouyna, IsabellePéhau-Arnaude, GérardCommere, Pierre-HenriNovault, SophieEne, Iuliana V.Nimrichter, LeonardoRodrigues, Marcio LourençoJanbon, Guilhem2023-10-16T16:18:48Z2023-10-16T16:18:48Z2023RIZZO, Juliana et al. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans. mBio. v. 14, n. 4, p. 1-22, 2023.2150-7511https://www.arca.fiocruz.br/handle/icict/60811porPublic Library of ScienceCoregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformansinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstitut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Institut Pasteur. Paris, France. / Université Paris Cité. USR 3756 IP CNRS, HUB Bioinformatique et Biostatistique. Paris, France.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Centre National de Référence Mycoses Invasives et Antifongiques. Groupe de recherche Mycologie Translationnelle. Département de Mycologie. Paris, France. / Laboratoire de parasitologie-mycologie, AP-HP. Hôpital Saint-Louis. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Centre National de Référence Mycoses Invasives et Antifongiques. Groupe de recherche Mycologie Translationnelle. Département de Mycologie. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Plateforme de Bio-Imagerie Ultrastructurale. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Cytometry and Biomarkers. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Cytometry and Biomarkers. Paris, France.Institut Pasteur. Paris, France. / Université Paris Cité. Fungal Heterogeneity Group. Paris, France.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France.Resistance to fluconazole (FLC), the most widely used antifungal drug, is typically achieved by altering the azole drug target and/or drug efflux pumps. Recent reports have suggested a link between vesicular trafficking and antifungal resistance. Here, we identified novel Cryptococcus neoformans regulators of extracellular vesicle (EV) biogenesis that impact FLC resistance. In particular, the transcription factor Hap2 does not affect the expression of the drug target or efflux pumps, yet it impacts the cellular sterol profile. Subinhibitory FLC concentrations also downregulate EV production. Moreover, in vitro spontaneous FLC-resistant colonies showed altered EV production, and the acquisition of FLC resistance was associated with decreased EV production in clinical isolates. Finally, the reversion of FLC resistance was associated with increased EV production. These data suggest a model in which fungal cells can regulate EV production in place of regulating the drug target gene expression as a first line of defense against antifungal assault in this fungal pathogen.Cryptococcus neoformansExtracellular VesiclesDrug Resistance, MicrobialFluconazoleTranscription FactorsCryptococcus neoformansVesículas ExtracelularesFarmacorresistencia MicrobianaFluconazolFactores de TranscripciónCryptococcus neoformansVésicules extracellulairesRésistance microbienne aux médicamentsFluconazoleFacteurs de transcriptionCryptococcus neoformansVesículas ExtracelularesResistência Microbiana a MedicamentosFluconazolFatores de Transcriçãoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.en_US.fl_str_mv |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| title |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| spellingShingle |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans Rizzo, Juliana Cryptococcus neoformans Extracellular Vesicles Drug Resistance, Microbial Fluconazole Transcription Factors Cryptococcus neoformans Vesículas Extracelulares Farmacorresistencia Microbiana Fluconazol Factores de Transcripción Cryptococcus neoformans Vésicules extracellulaires Résistance microbienne aux médicaments Fluconazole Facteurs de transcription Cryptococcus neoformans Vesículas Extracelulares Resistência Microbiana a Medicamentos Fluconazol Fatores de Transcrição |
| title_short |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| title_full |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| title_fullStr |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| title_full_unstemmed |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| title_sort |
Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans |
| author |
Rizzo, Juliana |
| author_facet |
Rizzo, Juliana Trottier, Adèle Moyrand, Frédérique Coppée, Jean-Yves Maufrais, Corinne Zimbres, Ana Claudia G. Dang, Thi Tuong Vi Alanio, Alexandre Desnos-Ollivier, Marie Mouyna, Isabelle Péhau-Arnaude, Gérard Commere, Pierre-Henri Novault, Sophie Ene, Iuliana V. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Janbon, Guilhem |
| author_role |
author |
| author2 |
Trottier, Adèle Moyrand, Frédérique Coppée, Jean-Yves Maufrais, Corinne Zimbres, Ana Claudia G. Dang, Thi Tuong Vi Alanio, Alexandre Desnos-Ollivier, Marie Mouyna, Isabelle Péhau-Arnaude, Gérard Commere, Pierre-Henri Novault, Sophie Ene, Iuliana V. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Janbon, Guilhem |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rizzo, Juliana Trottier, Adèle Moyrand, Frédérique Coppée, Jean-Yves Maufrais, Corinne Zimbres, Ana Claudia G. Dang, Thi Tuong Vi Alanio, Alexandre Desnos-Ollivier, Marie Mouyna, Isabelle Péhau-Arnaude, Gérard Commere, Pierre-Henri Novault, Sophie Ene, Iuliana V. Nimrichter, Leonardo Rodrigues, Marcio Lourenço Janbon, Guilhem |
| dc.subject.en.en_US.fl_str_mv |
Cryptococcus neoformans Extracellular Vesicles Drug Resistance, Microbial Fluconazole Transcription Factors |
| topic |
Cryptococcus neoformans Extracellular Vesicles Drug Resistance, Microbial Fluconazole Transcription Factors Cryptococcus neoformans Vesículas Extracelulares Farmacorresistencia Microbiana Fluconazol Factores de Transcripción Cryptococcus neoformans Vésicules extracellulaires Résistance microbienne aux médicaments Fluconazole Facteurs de transcription Cryptococcus neoformans Vesículas Extracelulares Resistência Microbiana a Medicamentos Fluconazol Fatores de Transcrição |
| dc.subject.es.en_US.fl_str_mv |
Cryptococcus neoformans Vesículas Extracelulares Farmacorresistencia Microbiana Fluconazol Factores de Transcripción |
| dc.subject.fr.en_US.fl_str_mv |
Cryptococcus neoformans Vésicules extracellulaires Résistance microbienne aux médicaments Fluconazole Facteurs de transcription |
| dc.subject.decs.en_US.fl_str_mv |
Cryptococcus neoformans Vesículas Extracelulares Resistência Microbiana a Medicamentos Fluconazol Fatores de Transcrição |
| description |
Institut Pasteur. Paris, France. / Université Paris Cité. Unité Biologie des ARN des Pathogènes Fongiques. Paris, France. / Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil. |
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2023 |
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2023-10-16T16:18:48Z |
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2023-10-16T16:18:48Z |
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2023 |
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info:eu-repo/semantics/article |
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RIZZO, Juliana et al. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans. mBio. v. 14, n. 4, p. 1-22, 2023. |
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https://www.arca.fiocruz.br/handle/icict/60811 |
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2150-7511 |
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RIZZO, Juliana et al. Coregulation of extracellular vesicle production and fluconazole susceptibility in Cryptococcus neoformans. mBio. v. 14, n. 4, p. 1-22, 2023. 2150-7511 |
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