Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil

Detalhes bibliográficos
Autor(a) principal: Freire, Ana Karla Lima
Data de Publicação: 2012
Outros Autores: Bentes, Amaury dos Santos, Sampaio, Ivanete de Lima, Matsuura, Ani Beatriz Jackisch, Ogusku, Maurício Morishi, Salem, Julia Ignez, Wanke, Bodo, Souza, João Vicente Braga de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36640
Resumo: 2020-10-22
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spelling Freire, Ana Karla LimaBentes, Amaury dos SantosSampaio, Ivanete de LimaMatsuura, Ani Beatriz JackischOgusku, Maurício MorishiSalem, Julia IgnezWanke, BodoSouza, João Vicente Braga de2019-10-22T14:31:49Z2019-10-22T14:31:49Z2012FREIRE, Ana Karla Lima. Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facilityin the state of Amazonas-Brazil. Mycoses, v. 55, p. 145-150, 2012.0933-7407https://www.arca.fiocruz.br/handle/icict/3664010.1111/j.1439-0507.2012.02173.x1439-0507engWileyMolecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-10-22Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Laboratório de Micologia. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micobacteriologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Laboratório de Micologia. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micobacteriologia. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micobacteriologia. Manaus, AM, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Laboratório de Micologia. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Serviço de Micologia. Rio de Janeiro, RJ, Brasil.Fundação de Medicina Tropical Dr. Heitor Vieira Dourado. Laboratório de Micologia. Manaus, AM, Brasil / Universidade do Estado do Amazonas. Manaus, AM, Brasil / Instituto Nacional de Pesquisas da Amazônia. Laboratório de Micobacteriologia. Manaus, AM, Brasil.As there are four major molecular types of Cryptococcus neoformans(VNI, VNII, VNIIIand VNIV) and four molecular types of Cryptococcus gattii (VGI, VGII, VGIII and VGIV), it is important to identify the specific groups causing cryptococcosis in different geographical regions. Here, we investigated the molecular types of 57 cryptococcal isolates from patients in a tertiary care hospital in the state of Amazonas, Brazil, between 2006 and 2010. The isolates were characterised by PCR fingerprinting using the M13 minisatellite and confirmed by URA5-RFLP analysis, and the presence of specific genes from the mating type locus (MATα and MATa) of these species was analysed by PCR. Most of the patients were male (66.7%), between 16 and 30 years of age (51.7%), and HIV-positive (75.0%). Most isolates were collected from cerebrospinal fluid samples (71.7%). Most of the C. neoformans isolates (n= 40) were characterised as members of the VNI molecular group (n= 39), a unique isolate was characterised as VNII whereas all isolates of C. gattii (n= 17) were members of the VGII molecular group. With regard to mating types, 55 isolates were type "a", and only two were type "a". This study revealed the prevalence of the VNI molecular group and provides the first reported observation of the VNII molecular group in the northern region of Brazil.CryptococcusGenotypingPCR-fingerprintingM13URA5-RFLPITS1 RFLP analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36640/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Freire_Ana_etal_INI_2012.pdfve_Freire_Ana_etal_INI_2012.pdfapplication/pdf161217https://www.arca.fiocruz.br/bitstream/icict/36640/2/ve_Freire_Ana_etal_INI_2012.pdf0442aac1149250f72cd0ca9c5fc2c780MD52TEXTve_Freire_Ana_etal_INI_2012.pdf.txtve_Freire_Ana_etal_INI_2012.pdf.txtExtracted texttext/plain25380https://www.arca.fiocruz.br/bitstream/icict/36640/3/ve_Freire_Ana_etal_INI_2012.pdf.txt155a505878306fd2db86f66c67f84e35MD53icict/366402021-03-24 16:32:38.042oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352021-03-24T19:32:38Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
title Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
spellingShingle Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
Freire, Ana Karla Lima
Cryptococcus
Genotyping
PCR-fingerprinting
M13
URA5-RFLP
ITS1 RFLP analysis
title_short Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
title_full Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
title_fullStr Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
title_full_unstemmed Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
title_sort Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facility in the state of Amazonas-Brazil
author Freire, Ana Karla Lima
author_facet Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Sampaio, Ivanete de Lima
Matsuura, Ani Beatriz Jackisch
Ogusku, Maurício Morishi
Salem, Julia Ignez
Wanke, Bodo
Souza, João Vicente Braga de
author_role author
author2 Bentes, Amaury dos Santos
Sampaio, Ivanete de Lima
Matsuura, Ani Beatriz Jackisch
Ogusku, Maurício Morishi
Salem, Julia Ignez
Wanke, Bodo
Souza, João Vicente Braga de
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freire, Ana Karla Lima
Bentes, Amaury dos Santos
Sampaio, Ivanete de Lima
Matsuura, Ani Beatriz Jackisch
Ogusku, Maurício Morishi
Salem, Julia Ignez
Wanke, Bodo
Souza, João Vicente Braga de
dc.subject.en.pt_BR.fl_str_mv Cryptococcus
Genotyping
PCR-fingerprinting
M13
URA5-RFLP
ITS1 RFLP analysis
topic Cryptococcus
Genotyping
PCR-fingerprinting
M13
URA5-RFLP
ITS1 RFLP analysis
description 2020-10-22
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-10-22T14:31:49Z
dc.date.available.fl_str_mv 2019-10-22T14:31:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FREIRE, Ana Karla Lima. Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facilityin the state of Amazonas-Brazil. Mycoses, v. 55, p. 145-150, 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36640
dc.identifier.issn.pt_BR.fl_str_mv 0933-7407
dc.identifier.doi.none.fl_str_mv 10.1111/j.1439-0507.2012.02173.x
dc.identifier.eissn.none.fl_str_mv 1439-0507
identifier_str_mv FREIRE, Ana Karla Lima. Molecular characterisation of the causative agents of Cryptococcosis in patients of a tertiary healthcare facilityin the state of Amazonas-Brazil. Mycoses, v. 55, p. 145-150, 2012.
0933-7407
10.1111/j.1439-0507.2012.02173.x
1439-0507
url https://www.arca.fiocruz.br/handle/icict/36640
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/36640/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/36640/2/ve_Freire_Ana_etal_INI_2012.pdf
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