The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets

Detalhes bibliográficos
Autor(a) principal: Santos, Ana Paula dos
Data de Publicação: 2007
Outros Autores: Bertho, Álvaro Luiz, Martins, Reinaldo de Menezes, Marcovistz, Rugimar
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1590/S0074-02762007000100020
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32123
Resumo: Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Ana Paula dosBertho, Álvaro LuizMartins, Reinaldo de MenezesMarcovistz, Rugimar2019-03-15T18:18:15Z2019-03-15T18:18:15Z2007SANTOS, Ana Paula dos et al . The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 1, p. 117-120, Feb. 2007.1678-8060https://www.arca.fiocruz.br/handle/icict/3212310.1590/S0074-02762007000100020engThe sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsetsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Imunologia. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.The objective of this paper is to propose a protocol to analyze blood samples in yellow fever 17DD vaccinated which developed serious adverse events. We investigated whether or not the time between sample collection and sample processing could interfere in lymphocyte subset percentage, for it is often impossible to analyze blood samples immediately after collection due to transport delay from collection places to the flow cytometry facility. CD4+CD38+ T, CD8+CD38+ T, CD3+ T, CD19+ B lymphocyte subsets were analyzed by flow cytometry in nine healthy volunteers immediately after blood collection and after intervals of 24 and 48 h. The whole blood lysis method and gradient sedimentation by Histopaque were applied to isolate peripheral blood mononuclear cells for flow cytometry analyses. With the lysis method, there was no significant change in lymphocyte subset percentage between the two time intervals (24 and 48 h). In contrast, when blood samples were processed by Histopaque gradient sedimentation, time intervals for sample processing influenced the percentage in T lymphocyte subsets but not in B cells. From the results obtained, we could conclude that the whole blood lysis method is more appropriate than gradient sedimentation by Histopaque for immunophenotyping of blood samples collected after serious adverse events, due to less variation in the lymphocyte subset levels with respect to the time factor.Yellow feverFlow cytometryLymphocyte subsetsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/32123/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALF96-5660.pdfapplication/pdf206933https://www.arca.fiocruz.br/bitstream/icict/32123/2/F96-5660.pdfc1a69ee894b92a6b6db6f3c461f12b1cMD52TEXTF96-5660.pdf.txtF96-5660.pdf.txtExtracted texttext/plain14555https://www.arca.fiocruz.br/bitstream/icict/32123/3/F96-5660.pdf.txtd01f1e9396237fa2c3b62dbaab17fc0eMD53icict/321232019-04-04 02:05:10.871oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-04-04T05:05:10Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
title The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
spellingShingle The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
Santos, Ana Paula dos
Yellow fever
Flow cytometry
Lymphocyte subsets
title_short The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
title_full The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
title_fullStr The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
title_full_unstemmed The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
title_sort The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets
author Santos, Ana Paula dos
author_facet Santos, Ana Paula dos
Bertho, Álvaro Luiz
Martins, Reinaldo de Menezes
Marcovistz, Rugimar
author_role author
author2 Bertho, Álvaro Luiz
Martins, Reinaldo de Menezes
Marcovistz, Rugimar
author2_role author
author
author
dc.contributor.author.fl_str_mv Santos, Ana Paula dos
Bertho, Álvaro Luiz
Martins, Reinaldo de Menezes
Marcovistz, Rugimar
dc.subject.en.pt_BR.fl_str_mv Yellow fever
Flow cytometry
Lymphocyte subsets
topic Yellow fever
Flow cytometry
Lymphocyte subsets
description Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2019-03-15T18:18:15Z
dc.date.available.fl_str_mv 2019-03-15T18:18:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Ana Paula dos et al . The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 1, p. 117-120, Feb. 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32123
dc.identifier.issn.pt_BR.fl_str_mv 1678-8060
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762007000100020
identifier_str_mv SANTOS, Ana Paula dos et al . The sample processing time interval as an influential factor in flow cytometry analysis of lymphocyte subsets. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 1, p. 117-120, Feb. 2007.
1678-8060
10.1590/S0074-02762007000100020
url https://www.arca.fiocruz.br/handle/icict/32123
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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