Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19544 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, Brasil |
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Sato, Camila MassaeSanchez, Maria Carmen ArroyoCeleste, Beatriz JulietaDuthie, Malcolm S.Guderian, JeffreyReed, Steven G.de Brito, Maria Edileuza FelintoCampos, Marliane Batistade Souza Encarnação, Helia ValeriaGuerra, Jorgede Mesquita, Tirza Gabrielle RamosPinheiro, Suzana KanawatiRamasawmy, RajendranathSilveira, Fernando Tobiasde Assis Souza, MarinaGoto, Hiro2017-06-27T19:33:55Z2017-06-27T19:33:55Z2017SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16.1098-660Xhttps://www.arca.fiocruz.br/handle/icict/1954410.1128/JCM.01904-16engUse of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Speciesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, BrasilAmerican tegumentary leishmaniasis (ATL) (also known as cutaneous leishmaniasis [CL]) is caused by various species of protozoa of the genus Leishmania The diagnosis is achieved on a clinical, epidemiological, and pathological basis, supported by positive parasitological exams and demonstration of leishmanin delayed-type hypersensitivity. Serological assays are not routinely used in the diagnosis because many are considered to have low sensitivity and the particular Leishmania species causing the disease can lead to variable performance. In the present study, we generated recombinant versions of two highly conserved Leishmania proteins, Leishmania (Viannia) braziliensis-derived Lb8E and Lb6H, and evaluated both in enzyme-linked immunosorbent assays (ELISA). Recombinant Lb6H (rLb6H) had better performance and reacted with 100.0% of the ATL and 89.4% of the VL samples. These reactions with rLb6H were highly specific (98.5%) when compared against those for samples from healthy control individuals. We then assessed rLb6H against sera from ATL patients infected with different species of Leishmania prevalent in Brazil [Leishmania (Leishmania) amazonensis, L (Viannia) braziliensis, and L (V) guyanensis] and samples from patients with other infectious diseases. In analyses of 500 sera, ELISA using rLb6H detected all 219 ATL samples (sensitivity of 100.0%) with an overall specificity of 93.9% (considering healthy individuals and other infectious diseases patients). Only a minority of samples from Chagas disease patients possessed antibodies against rLb6H, and all of these responses were low (with a highest reactivity index of 2.2). Taken together, our data support further evaluation of rLb6H and the potential for its routine use in the serological diagnosis of ATL.Leishmaniasis, CutaneousdiagnosticsELISAimmunoserologyrecombinant antigentegumentary leishmaniasisLeishmaniose CutâneaProteínas Recombinantesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19544/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINAL27927927 2017 sat-use.oa.pdf27927927 2017 sat-use.oa.pdfapplication/pdf548059https://www.arca.fiocruz.br/bitstream/icict/19544/2/27927927%202017%20sat-use.oa.pdf203532deb5164f6376996aeeadd65c0fMD52TEXT27927927 2017 sat-use.oa.pdf.txt27927927 2017 sat-use.oa.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| spellingShingle |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species Sato, Camila Massae Leishmaniasis, Cutaneous diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis Leishmaniose Cutânea Proteínas Recombinantes |
| title_short |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_full |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_fullStr |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_full_unstemmed |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| title_sort |
Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species |
| author |
Sato, Camila Massae |
| author_facet |
Sato, Camila Massae Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. de Brito, Maria Edileuza Felinto Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| author_role |
author |
| author2 |
Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. de Brito, Maria Edileuza Felinto Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| author2_role |
author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Sato, Camila Massae Sanchez, Maria Carmen Arroyo Celeste, Beatriz Julieta Duthie, Malcolm S. Guderian, Jeffrey Reed, Steven G. de Brito, Maria Edileuza Felinto Campos, Marliane Batista de Souza Encarnação, Helia Valeria Guerra, Jorge de Mesquita, Tirza Gabrielle Ramos Pinheiro, Suzana Kanawati Ramasawmy, Rajendranath Silveira, Fernando Tobias de Assis Souza, Marina Goto, Hiro |
| dc.subject.en.en.fl_str_mv |
Leishmaniasis, Cutaneous diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis |
| topic |
Leishmaniasis, Cutaneous diagnostics ELISA immunoserology recombinant antigen tegumentary leishmaniasis Leishmaniose Cutânea Proteínas Recombinantes |
| dc.subject.decs.pt_BR.fl_str_mv |
Leishmaniose Cutânea Proteínas Recombinantes |
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Fundação Oswaldo Cruz. Instituto Aggeu Magalhães, Recife, PE, Brasil |
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2017 |
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2017-06-27T19:33:55Z |
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2017-06-27T19:33:55Z |
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2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16. |
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https://www.arca.fiocruz.br/handle/icict/19544 |
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1098-660X |
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10.1128/JCM.01904-16 |
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SATO, C. M. et al. Use of Recombinant Antigens for Sensitive Serodiagnosis of American Tegumentary Leishmaniasis Caused by Different Leishmania Species. Journal of Clinical Microbiology, v. 55, n. 2, p. 495–503, fev. 2017. DOI 10.1128/JCM.01904-16. 1098-660X 10.1128/JCM.01904-16 |
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