In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression

Detalhes bibliográficos
Autor(a) principal: Hhamedi, Afsaneh
Data de Publicação: 2015
Outros Autores: Botelho, Larisse, Britto, Constança, Fragoso, Stenio Perdigão, Umaki, Adriana Castilhos Souza, Goldenberg, Samuel, Bottu, Guy, Salmon, Didier
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15504
Resumo: CNPq
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spelling Hhamedi, AfsanehBotelho, LarisseBritto, ConstançaFragoso, Stenio PerdigãoUmaki, Adriana Castilhos SouzaGoldenberg, SamuelBottu, GuySalmon, Didier2016-08-29T17:51:17Z2016-08-29T17:51:17Z2015HAMEDI, Afsaneh et al. In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of adenylyl cyclase expression. Molecular & Biochemical Parasitology, v. 200, p. 9-18, 2015.0166-6851https://www.arca.fiocruz.br/handle/icict/15504CNPqFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Université Libre de Bruxelles. Campus de la Plaine. Brussels, Belgium.Universidade Federal do Estado do Rio de Janeiro. Centro de Ciências e da Saúde. Instituto de Bioquímica Médica. Rio de Janeiro, RJ, Brasil.The Trypanosoma cruzi adenylyl cyclase (AC) multigene family encodes different isoforms (around 15) sharing a variable large N-terminal domain, which is extracellular and receptor-like, followed by a transmembrane helix and a conserved C-terminal catalytic domain. It was proposed that these key enzymes in the cAMP signalling pathway allow the parasite to sense its changing extracellular milieu in order to rapidly adapt to its new environment, which is generally achieved through a differentiation process. One of the critical differentiation events the parasitic protozoan T. cruzi undergoes during its life cycle, known as metacyclogenesis, occurs in the digestive tract of the insect and corresponds to the differentiation from noninfective epimastigotes to infective metacyclic trypomastigote forms. By in vitro monitoring the activity of AC during metacyclogenesis, we showed that both the activity of AC and the intracellular cAMP content follow a similar pattern of transient stimulation in a two-step process, with a first activation peak occurring during the first hours of nutritional stress and a second peak between 6 and 48 h, corresponding to the cellular adhesion. During this differentiation process, a general mechanism of upregulation of AC expression of both mRNA and protein is triggered and in particular for a major subclass of these enzymes that are present in various gene copies commonly associated to the THT gene clusters. Although the scattered genome distribution of these gene copies is rather unusual in trypanosomatids and seems to be a recent acquisition in the evolution of the T. cruzi clade, their encoded product redistributed on the flagellum of the parasite upon differentiation could be important to sense the extracellular milieu.porIn vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expressioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleAdenylyl cyclaseMetacyclogenesisParasite differentiationT. cruzicAMPinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15504/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALIn vitro metacyclogenesis of Trypanosoma cruzi induced by starvation.pdfIn vitro metacyclogenesis of Trypanosoma cruzi induced by starvation.pdfapplication/pdf3792392https://www.arca.fiocruz.br/bitstream/icict/15504/2/In%20vitro%20metacyclogenesis%20of%20Trypanosoma%20cruzi%20induced%20by%20starvation.pdf0bdfbe5e3cebaa60e8a1cef0ccca3acaMD52TEXTIn vitro metacyclogenesis of Trypanosoma cruzi induced by starvation.pdf.txtIn vitro metacyclogenesis of Trypanosoma cruzi induced by starvation.pdf.txtExtracted texttext/plain61134https://www.arca.fiocruz.br/bitstream/icict/15504/3/In%20vitro%20metacyclogenesis%20of%20Trypanosoma%20cruzi%20induced%20by%20starvation.pdf.txt09ad18d88de4c38b6557f5611ede8bb5MD53icict/155042019-10-31 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dc.title.pt_BR.fl_str_mv In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
title In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
spellingShingle In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
Hhamedi, Afsaneh
Adenylyl cyclase
Metacyclogenesis
Parasite differentiation
T. cruzi
cAMP
title_short In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
title_full In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
title_fullStr In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
title_full_unstemmed In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
title_sort In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of Idenylyl cyclase expression
author Hhamedi, Afsaneh
author_facet Hhamedi, Afsaneh
Botelho, Larisse
Britto, Constança
Fragoso, Stenio Perdigão
Umaki, Adriana Castilhos Souza
Goldenberg, Samuel
Bottu, Guy
Salmon, Didier
author_role author
author2 Botelho, Larisse
Britto, Constança
Fragoso, Stenio Perdigão
Umaki, Adriana Castilhos Souza
Goldenberg, Samuel
Bottu, Guy
Salmon, Didier
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Hhamedi, Afsaneh
Botelho, Larisse
Britto, Constança
Fragoso, Stenio Perdigão
Umaki, Adriana Castilhos Souza
Goldenberg, Samuel
Bottu, Guy
Salmon, Didier
dc.subject.en.pt_BR.fl_str_mv Adenylyl cyclase
Metacyclogenesis
Parasite differentiation
T. cruzi
cAMP
topic Adenylyl cyclase
Metacyclogenesis
Parasite differentiation
T. cruzi
cAMP
description CNPq
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-08-29T17:51:17Z
dc.date.available.fl_str_mv 2016-08-29T17:51:17Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv HAMEDI, Afsaneh et al. In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of adenylyl cyclase expression. Molecular & Biochemical Parasitology, v. 200, p. 9-18, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15504
dc.identifier.issn.pt_BR.fl_str_mv 0166-6851
identifier_str_mv HAMEDI, Afsaneh et al. In vitro metacyclogenesis of Trypanosoma cruzi induced by starvation correlates with a transient adenylyl cyclase stimulation as well as with a constitutive upregulation of adenylyl cyclase expression. Molecular & Biochemical Parasitology, v. 200, p. 9-18, 2015.
0166-6851
url https://www.arca.fiocruz.br/handle/icict/15504
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15504/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15504/2/In%20vitro%20metacyclogenesis%20of%20Trypanosoma%20cruzi%20induced%20by%20starvation.pdf
https://www.arca.fiocruz.br/bitstream/icict/15504/3/In%20vitro%20metacyclogenesis%20of%20Trypanosoma%20cruzi%20induced%20by%20starvation.pdf.txt
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