Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome

Detalhes bibliográficos
Autor(a) principal: Almeida, Viviane de Sousa Moreira
Data de Publicação: 2020
Outros Autores: Azevedo, Jailton, Leal, Helena Ferreira, Queiroz, Artur Trancoso Lopo de, Silva Filho, Hermes Pedreira da, Reis, Joice Neves
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44289
Resumo: CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior- VSMA), FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia-HFL, JNR), INPRA (Instituto Nacional de Pesquisa em Resistência Antimicrobiana-JNR), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul) for their financial support. ATLQ acknowledges financial support from the program Inova Fiocruz (Project number VPPIS-001-FIO18).
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spelling Almeida, Viviane de Sousa MoreiraAzevedo, JailtonLeal, Helena FerreiraQueiroz, Artur Trancoso Lopo deSilva Filho, Hermes Pedreira daReis, Joice Neves2020-10-31T12:19:04Z2020-10-31T12:19:04Z2020ALMEIDA, Viviane de Sousa Moreira et al. Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome. Plos One, p. 1-17, Sept. 29, 2020.1932-6203https://www.arca.fiocruz.br/handle/icict/4428910.1371/journal.pone.0239664CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior- VSMA), FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia-HFL, JNR), INPRA (Instituto Nacional de Pesquisa em Resistência Antimicrobiana-JNR), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul) for their financial support. ATLQ acknowledges financial support from the program Inova Fiocruz (Project number VPPIS-001-FIO18).Federal University of Bahia. School of Pharmacy. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Recôncavo da Bahia. Health Science Center. Santo Antônio de Jesus, BA, Brazil.Federal University of Bahia. School of Pharmacy. Salvador, BA, Brazil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.This study aims to describe the oral microbiome diversity and prevalence of ARGs in periodontal health and disease. Background The human oral cavity harbors a complex microbial community known as the oral microbiome. These organisms are regularly exposed to selective pressures, such as the usage of antibiotics, which drive evolution and acquisition of antibiotic resistance genes (ARGs). Resistance among oral bacteria jeopardizes not only antibiotic therapy for oral infections, but also extra-oral infections caused by bacterial translocation. Methods We carried out a cross-sectional investigation. Saliva and subgingival plaque samples were collected during a clinical exam. 16S rRNA gene sequencing was performed to assess microbial diversity. Resistance genes were identified through PCR assays. Results Of the 110 participants, only 22.7% had healthy periodontium, while the majority was diagnosed with gingivitis (55.4%) and chronic periodontitis (21.8%). The composition of the oral microbiota differed from healthy and diseased samples, being Streptococcus spp. and Rothia spp. predominant in periodontal disease. Regarding ARGs, 80 (72.7%) samples were positive for at least one of genes screened, erm being the most frequent variant (58.2%), followed by blaTEM (16.4%), mecA (2.7%), pbp2b and aac(6 ’) (1.8%). Neither genes coding resistance to carbapenems nor metronidazole were detected. Conclusions Our findings indicate that there are no significant differences in terms of taxonomic enrichment between healthy and diseased oral microbiomes. However, samples retrieved from healthy patients had a more diverse microbial community, whereas diseased samples have lower taxonomic diversity. We have also identified clinically relevant ARGs, providing baseline information to guide antibiotic prescription in dentistry.engPublic Library of ScienceAntibacterianosResistência Microbiana a MedicamentosMicrobiotaPeriodontiteDoenças periodontaisAnti-Bacterial AgentsDrug Resistance, MicrobialMicrobiotaPeriodontitisPeriodontal DiseasesBacterial diversity and prevalence of antibiotic resistance genes in the oral microbiomeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44289/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAlmeida, Viviane de Sousa Moreira Bacterial....pdfAlmeida, Viviane de Sousa Moreira 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dc.title.pt_BR.fl_str_mv Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
title Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
spellingShingle Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
Almeida, Viviane de Sousa Moreira
Antibacterianos
Resistência Microbiana a Medicamentos
Microbiota
Periodontite
Doenças periodontais
Anti-Bacterial Agents
Drug Resistance, Microbial
Microbiota
Periodontitis
Periodontal Diseases
title_short Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
title_full Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
title_fullStr Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
title_full_unstemmed Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
title_sort Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome
author Almeida, Viviane de Sousa Moreira
author_facet Almeida, Viviane de Sousa Moreira
Azevedo, Jailton
Leal, Helena Ferreira
Queiroz, Artur Trancoso Lopo de
Silva Filho, Hermes Pedreira da
Reis, Joice Neves
author_role author
author2 Azevedo, Jailton
Leal, Helena Ferreira
Queiroz, Artur Trancoso Lopo de
Silva Filho, Hermes Pedreira da
Reis, Joice Neves
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Viviane de Sousa Moreira
Azevedo, Jailton
Leal, Helena Ferreira
Queiroz, Artur Trancoso Lopo de
Silva Filho, Hermes Pedreira da
Reis, Joice Neves
dc.subject.other.pt_BR.fl_str_mv Antibacterianos
Resistência Microbiana a Medicamentos
Microbiota
Periodontite
Doenças periodontais
topic Antibacterianos
Resistência Microbiana a Medicamentos
Microbiota
Periodontite
Doenças periodontais
Anti-Bacterial Agents
Drug Resistance, Microbial
Microbiota
Periodontitis
Periodontal Diseases
dc.subject.en.pt_BR.fl_str_mv Anti-Bacterial Agents
Drug Resistance, Microbial
Microbiota
Periodontitis
Periodontal Diseases
description CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior- VSMA), FAPESB (Fundação de Amparo à Pesquisa do Estado da Bahia-HFL, JNR), INPRA (Instituto Nacional de Pesquisa em Resistência Antimicrobiana-JNR), CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), and FAPERGS (Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul) for their financial support. ATLQ acknowledges financial support from the program Inova Fiocruz (Project number VPPIS-001-FIO18).
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-10-31T12:19:04Z
dc.date.available.fl_str_mv 2020-10-31T12:19:04Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Viviane de Sousa Moreira et al. Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome. Plos One, p. 1-17, Sept. 29, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44289
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0239664
identifier_str_mv ALMEIDA, Viviane de Sousa Moreira et al. Bacterial diversity and prevalence of antibiotic resistance genes in the oral microbiome. Plos One, p. 1-17, Sept. 29, 2020.
1932-6203
10.1371/journal.pone.0239664
url https://www.arca.fiocruz.br/handle/icict/44289
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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