Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes

Detalhes bibliográficos
Autor(a) principal: Oliveira, Sabrina B
Data de Publicação: 2011
Outros Autores: Ibraim, Izabela Coimbra, Tadei, Wanderli Pedro, Ruiz, Jeronimo Conceição, Nahum, Laila Alves, Brito, Cristiana Ferreira Alves de, Moreira, Luciano Andrade
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8997
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.
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spelling Oliveira, Sabrina BIbraim, Izabela CoimbraTadei, Wanderli PedroRuiz, Jeronimo ConceiçãoNahum, Laila AlvesBrito, Cristiana Ferreira Alves deMoreira, Luciano Andrade2014-11-28T12:47:36Z2014-11-28T12:47:36Z2011OLIVEIRA, Sabrina B et al. Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes. MALARIA J, v. 10, 21, 2011.1475-2875https://www.arca.fiocruz.br/handle/icict/899710.1186/1475-2875-10-21engBioMed CentralIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Malária e Dengue. Manaus, AM, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.BACKGROUND: Malaria has a devastating impact on worldwide public health in many tropical areas. Studies on vector immunity are important for the overall understanding of the parasite-vector interaction and for the design of novel strategies to control malaria. A member of the fibrinogen-related protein family, fbn9, has been well studied in Anopheles gambiae and has been shown to be an important component of the mosquito immune system. However, little is known about this gene in neotropical anopheline species. METHODS: This article describes the identification and characterization of the fbn9 gene partial sequences from four species of neotropical anopheline primary and secondary vectors: Anopheles darlingi, Anopheles nuneztovari, Anopheles aquasalis, and Anopheles albitarsis (namely Anopheles marajoara). Degenerate primers were designed based on comparative analysis of publicly available Aedes aegypti and An. gambiae gene sequences and used to clone putative homologs in the neotropical species. Sequence comparisons and Bayesian phylogenetic analyses were then performed to better understand the molecular diversity of this gene in evolutionary distant anopheline species, belonging to different subgenera. RESULTS:Comparisons of the fbn9 gene sequences of the neotropical anophelines and their homologs in the An. gambiae complex (Gambiae complex) showed high conservation at the nucleotide and amino acid levels, although some sites show significant differentiation (non-synonymous substitutions). Furthermore, phylogenetic analysis of fbn9 nucleotide sequences showed that neotropical anophelines and African mosquitoes form two well-supported clades, mirroring their separation into two different subgenera. CONCLUSIONS: The present work adds new insights into the conserved role of fbn9 in insect immunity in a broader range of anopheline species and reinforces the possibility of manipulating mosquito immunity to design novel pathogen control strategies.Amino Acid SequenceAnopheles/classificationAnopheles/immunologyMalaria/parasitologyGenes, InsectSequence Analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8997/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes.pdfIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes.pdfapplication/pdf1829508https://www.arca.fiocruz.br/bitstream/icict/8997/2/Identification%20of%20a%20fibrinogen-related%20protein%20%28FBN9%29%20gene%20in%20neotropical%20anopheline%20mosquitoes.pdf753116976a1d1b7047fb555f68edc6a3MD52TEXTIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes.pdf.txtIdentification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes.pdf.txtExtracted texttext/plain33465https://www.arca.fiocruz.br/bitstream/icict/8997/3/Identification%20of%20a%20fibrinogen-related%20protein%20%28FBN9%29%20gene%20in%20neotropical%20anopheline%20mosquitoes.pdf.txt033efba3b2203f928f54b0767d7d9f81MD53icict/89972019-06-19 10:11:35.64oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:11:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
title Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
spellingShingle Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
Oliveira, Sabrina B
Amino Acid Sequence
Anopheles/classification
Anopheles/immunology
Malaria/parasitology
Genes, Insect
Sequence Analysis
title_short Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
title_full Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
title_fullStr Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
title_full_unstemmed Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
title_sort Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes
author Oliveira, Sabrina B
author_facet Oliveira, Sabrina B
Ibraim, Izabela Coimbra
Tadei, Wanderli Pedro
Ruiz, Jeronimo Conceição
Nahum, Laila Alves
Brito, Cristiana Ferreira Alves de
Moreira, Luciano Andrade
author_role author
author2 Ibraim, Izabela Coimbra
Tadei, Wanderli Pedro
Ruiz, Jeronimo Conceição
Nahum, Laila Alves
Brito, Cristiana Ferreira Alves de
Moreira, Luciano Andrade
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, Sabrina B
Ibraim, Izabela Coimbra
Tadei, Wanderli Pedro
Ruiz, Jeronimo Conceição
Nahum, Laila Alves
Brito, Cristiana Ferreira Alves de
Moreira, Luciano Andrade
dc.subject.en.pt_BR.fl_str_mv Amino Acid Sequence
Anopheles/classification
Anopheles/immunology
Malaria/parasitology
Genes, Insect
Sequence Analysis
topic Amino Acid Sequence
Anopheles/classification
Anopheles/immunology
Malaria/parasitology
Genes, Insect
Sequence Analysis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Malária. Belo Horizonte, MG, Brazil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-11-28T12:47:36Z
dc.date.available.fl_str_mv 2014-11-28T12:47:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv OLIVEIRA, Sabrina B et al. Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes. MALARIA J, v. 10, 21, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8997
dc.identifier.issn.none.fl_str_mv 1475-2875
dc.identifier.doi.none.fl_str_mv 10.1186/1475-2875-10-21
identifier_str_mv OLIVEIRA, Sabrina B et al. Identification of a fibrinogen-related protein (FBN9) gene in neotropical anopheline mosquitoes. MALARIA J, v. 10, 21, 2011.
1475-2875
10.1186/1475-2875-10-21
url https://www.arca.fiocruz.br/handle/icict/8997
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/8997/1/license.txt
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