High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56029 |
Resumo: | University of Notre Dame, Notre Dame, United States. |
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Vera-Arias, Claudia A.Holzschuh, AurelOduma, Colins OBadu, KingsleyAbdul-Hakim, MutalaYukich, JoshuaHetzel, Manuel WFakih, Bakar SAli, AbdullahFerreira, Marcelo ULadeia-Andrade, SimoneSáenz, Fabián EAfrane, YawZemene, EndalewYewhalaw, DelenasawKazura, James WYan, GuiyunKoepfli, Cristian2022-12-18T12:05:41Z2022-12-18T12:05:41Z2022VERA-ARIAS, Claudia A. et al. High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy. eLife, v.11, e72083, p. 1 - 16, June 2022.2050-084Xhttps://www.arca.fiocruz.br/handle/icict/5602910.7554/eLife.72083engBavesh D Kana, University of the Witwatersrand, South AfricaPlasmodium falciparumAlto rendimentoTipagem de deleção dos genes hrp2 e hrp3PCR digitalEficácia do teste de diagnósticoPara monitorar a malária rapidamentePlasmodium falciparumHigh-throughputHrp2 and hrp3 gene deletion typingDigital PCRTo monitor malaria rapidDiagnostic test efficacyHigh-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Notre Dame, Notre Dame, United States.University of Notre Dame, Notre Dame, United States / Swiss Tropical and Public Health Institute, Allschwil, Switzerland.Kenya Medical Research Institute-Centre for Global Health Research, Kisumu, Kenya / Department of Biochemistry and Molecular Biology, Egerton University, Nakuru, Kenya.Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.Tulane University, New Orleans, United States.Swiss Tropical and Public Health Institute, Allschwil, Switzerland / University of Basel, Basel, Switzerland.Swiss Tropical and Public Health Institute, Allschwil, Switzerland / University of Basel, Basel, Switzerland / Ifakara Health Institute, Dar es Salaam, United Republic of Tanzania.Zanzibar Malaria Elimination Programme, Zanzibar, Zanzibar, United Republic of Tanzania.Universidade de São Paulo. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Doenças Parasitárias. Rio de Janeiro, RJ, Brasil.Centro de Investigación para la Salud en América Latina, Facultad de Ciencias Exactas y Naturales, Pontificia Universidad Católica del Ecuador, Quito, Ecuador.Department of Medical Microbiology, University of Ghana, Accra, Ghana.Tropical and Infectious Diseases Research Center, Jimma University, Jimma, Ethiopia.Tropical and Infectious Diseases Research Center, Jimma University, Jimma, Ethiopia.Case Western Reserve University, Cleveland, United States.Program in Public Health, University of California, Irvine, Irvine, United States.University of Notre Dame, Notre Dame, United States.Most rapid diagnostic tests for Plasmodium falciparum malaria target the Histidine-Rich Proteins 2 and 3 (HRP2 and HRP3). Deletions of the hrp2 and hrp3 genes result in false-negative tests and are a threat for malaria control. A novel assay for molecular surveillance of hrp2/hrp3 deletions was developed based on droplet digital PCR (ddPCR). The assay quantifies hrp2, hrp3, and a control gene with very high accuracy. The theoretical limit of detection was 0.33 parasites/μl. The deletion was reliably detected in mixed infections with wild-type and hrp2-deleted parasites at a density of >100 parasites/reaction. For a side-by- side comparison with the conventional nested PCR (nPCR) assay, 248 samples were screened in triplicate by ddPCR and nPCR. No deletions were observed by ddPCR, while by nPCR hrp2 deletion was observed in 8% of samples. The ddPCR assay was applied to screen 830 samples from Kenya, Zanzibar/Tanzania, Ghana, Ethiopia, Brazil, and Ecuador. Pronounced differences in the prevalence of deletions were observed among sites, with more hrp3 than hrp2 deletions. In conclusion, the novel ddPCR assay minimizes the risk of false-negative results (i.e., hrp2 deletion observed when the sample is wild type), increases sensitivity, and greatly reduces the number of reactions that need to be run.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56029/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALClaudiaAVera-Arias_SimoneLAndrade_etal_IOC_2022.pdfClaudiaAVera-Arias_SimoneLAndrade_etal_IOC_2022.pdfapplication/pdf1153631https://www.arca.fiocruz.br/bitstream/icict/56029/2/ClaudiaAVera-Arias_SimoneLAndrade_etal_IOC_2022.pdfb37640a20293a2e98202e42041581846MD52icict/560292023-09-04 11:03:04.951oai:www.arca.fiocruz.br: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ório 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| dc.title.en_US.fl_str_mv |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| title |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| spellingShingle |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy Vera-Arias, Claudia A. Plasmodium falciparum Alto rendimento Tipagem de deleção dos genes hrp2 e hrp3 PCR digital Eficácia do teste de diagnóstico Para monitorar a malária rapidamente Plasmodium falciparum High-throughput Hrp2 and hrp3 gene deletion typing Digital PCR To monitor malaria rapid Diagnostic test efficacy |
| title_short |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| title_full |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| title_fullStr |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| title_full_unstemmed |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| title_sort |
High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy |
| author |
Vera-Arias, Claudia A. |
| author_facet |
Vera-Arias, Claudia A. Holzschuh, Aurel Oduma, Colins O Badu, Kingsley Abdul-Hakim, Mutala Yukich, Joshua Hetzel, Manuel W Fakih, Bakar S Ali, Abdullah Ferreira, Marcelo U Ladeia-Andrade, Simone Sáenz, Fabián E Afrane, Yaw Zemene, Endalew Yewhalaw, Delenasaw Kazura, James W Yan, Guiyun Koepfli, Cristian |
| author_role |
author |
| author2 |
Holzschuh, Aurel Oduma, Colins O Badu, Kingsley Abdul-Hakim, Mutala Yukich, Joshua Hetzel, Manuel W Fakih, Bakar S Ali, Abdullah Ferreira, Marcelo U Ladeia-Andrade, Simone Sáenz, Fabián E Afrane, Yaw Zemene, Endalew Yewhalaw, Delenasaw Kazura, James W Yan, Guiyun Koepfli, Cristian |
| author2_role |
author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Vera-Arias, Claudia A. Holzschuh, Aurel Oduma, Colins O Badu, Kingsley Abdul-Hakim, Mutala Yukich, Joshua Hetzel, Manuel W Fakih, Bakar S Ali, Abdullah Ferreira, Marcelo U Ladeia-Andrade, Simone Sáenz, Fabián E Afrane, Yaw Zemene, Endalew Yewhalaw, Delenasaw Kazura, James W Yan, Guiyun Koepfli, Cristian |
| dc.subject.other.en_US.fl_str_mv |
Plasmodium falciparum Alto rendimento Tipagem de deleção dos genes hrp2 e hrp3 PCR digital Eficácia do teste de diagnóstico Para monitorar a malária rapidamente |
| topic |
Plasmodium falciparum Alto rendimento Tipagem de deleção dos genes hrp2 e hrp3 PCR digital Eficácia do teste de diagnóstico Para monitorar a malária rapidamente Plasmodium falciparum High-throughput Hrp2 and hrp3 gene deletion typing Digital PCR To monitor malaria rapid Diagnostic test efficacy |
| dc.subject.en.en_US.fl_str_mv |
Plasmodium falciparum High-throughput Hrp2 and hrp3 gene deletion typing Digital PCR To monitor malaria rapid Diagnostic test efficacy |
| description |
University of Notre Dame, Notre Dame, United States. |
| publishDate |
2022 |
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2022-12-18T12:05:41Z |
| dc.date.available.fl_str_mv |
2022-12-18T12:05:41Z |
| dc.date.issued.fl_str_mv |
2022 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
VERA-ARIAS, Claudia A. et al. High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy. eLife, v.11, e72083, p. 1 - 16, June 2022. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/56029 |
| dc.identifier.issn.en_US.fl_str_mv |
2050-084X |
| dc.identifier.doi.none.fl_str_mv |
10.7554/eLife.72083 |
| identifier_str_mv |
VERA-ARIAS, Claudia A. et al. High-throughput Plasmodium falciparum hrp2 and hrp3 gene deletion typing by digital PCR to monitor malaria rapid diagnostic test efficacy. eLife, v.11, e72083, p. 1 - 16, June 2022. 2050-084X 10.7554/eLife.72083 |
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https://www.arca.fiocruz.br/handle/icict/56029 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Bavesh D Kana, University of the Witwatersrand, South Africa |
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Bavesh D Kana, University of the Witwatersrand, South Africa |
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