Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33978 |
Resumo: | Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
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Coelho, Rowena AlvesBrito-Santos, FábioFigueiredo-Carvalho, Maria Helena GaldinoSilva, Juliana Vitoria Dos SantosGutierrez-Galhardo, Maria ClaraValle, Antonio Carlos Francesconi doZancopé-Oliveira, Rosely MariaTrilles, LucianaMeyer, WielandFreitas, Dayvison Francis SaraivaAlmeida-Paes, Rodrigo2019-07-11T12:54:37Z2019-07-11T12:54:37Z2018COELHO, Rowena Alves et al. Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil. Plos Neglected Tropical Diseases, p. 1-15, July 2018.1935-2727https://www.arca.fiocruz.br/handle/icict/3397810.1371/journal.pntd.00066751935-2735engPublic Library of ScienceMolecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.University of Sydney. Marie Bashir Institute for Infectious Diseases and Biosecurity. Westmead Clinical School-Sydney Medical School. Sydney, Australia / Westmead Institute for Medical Research. Centre for Infectious Diseases and Microbiology. Molecular Mycology Research Laboratory. Sydney, Australia.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infecciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Background: Chromoblastomycosis (CBM) is a difficult-to-treat chronic subcutaneous mycosis. In Brazil, the main agent of this disease is Fonsecaea pedrosoi, which is phenotypically very similar to other Fonsecaea species, differing only genetically. The correct species identification is relevant since different species may differ in their epidemiologic aspects, clinical presentation, and treatment response. Methodology/Principal findings: Partial sequencing of the internal transcribed spacer (ITS) was used to identify twenty clinical isolates of Fonsecaea spp. Their in vitro antifungal susceptibility was determined using the broth microdilution method, according to the M38-A2 protocol. Amphotericin B (AMB), flucytosine (5FC), terbinafine (TRB), fluconazole (FLC), itraconazole (ITC), ketoconazole (KTC), posaconazole (POS), voriconazole (VRC), ravuconazole (RVC), caspofungin (CAS), and micafungin (MFG) were tested. The association between ITC/TRB, AMB/5FC, and ITC/CAS was studied by the checkerboard method to check synergism. The available patients’ data were correlated with the obtained laboratory results. Fonsecaea monophora (n = 10), F. pedrosoi (n = 5), and F. nubica (n = 5) were identified as CBM’ agents in the study. TRB and VRC were the drugs with the best in vitro activity with minimal inhibitory concentrations (MIC) lower than 0.25 mg/L. On the other hand, FLC, 5FC, AMB, and MFG showed high MICs. The AMB/5FC combination was synergistic for three F. monophora strains while the others were indifferent. Patients had moderate or severe CBM, and ITC therapy was not sufficient for complete cure in most of the cases, requiring adjuvant surgical approaches. Conclusions/Significance: F. monophora, the second most frequent Fonsecaea species in South America, predominated in patients raised and born in Rio de Janeiro, Brazil, without cerebral involvement in these cases. TRB, VRC, and the AMB/5FC combination should be further investigated as a treatment option for CBM.Fonsecaea spp.Chromoblastomycosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/33978/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Coelho_Rowena_etal_INI_2018.pdfve_Coelho_Rowena_etal_INI_2018.pdfapplication/pdf2052752https://www.arca.fiocruz.br/bitstream/icict/33978/2/ve_Coelho_Rowena_etal_INI_2018.pdf55f33c44bb5919d62c69584c04e2be6dMD52TEXTve_Coelho_Rowena_etal_INI_2018.pdf.txtve_Coelho_Rowena_etal_INI_2018.pdf.txtExtracted texttext/plain57035https://www.arca.fiocruz.br/bitstream/icict/33978/3/ve_Coelho_Rowena_etal_INI_2018.pdf.txta31af8374f3688ac1bb4f13aabef6acaMD53icict/339782019-07-12 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| dc.title.pt_BR.fl_str_mv |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| title |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| spellingShingle |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil Coelho, Rowena Alves Fonsecaea spp. Chromoblastomycosis |
| title_short |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| title_full |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| title_fullStr |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| title_full_unstemmed |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| title_sort |
Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil |
| author |
Coelho, Rowena Alves |
| author_facet |
Coelho, Rowena Alves Brito-Santos, Fábio Figueiredo-Carvalho, Maria Helena Galdino Silva, Juliana Vitoria Dos Santos Gutierrez-Galhardo, Maria Clara Valle, Antonio Carlos Francesconi do Zancopé-Oliveira, Rosely Maria Trilles, Luciana Meyer, Wieland Freitas, Dayvison Francis Saraiva Almeida-Paes, Rodrigo |
| author_role |
author |
| author2 |
Brito-Santos, Fábio Figueiredo-Carvalho, Maria Helena Galdino Silva, Juliana Vitoria Dos Santos Gutierrez-Galhardo, Maria Clara Valle, Antonio Carlos Francesconi do Zancopé-Oliveira, Rosely Maria Trilles, Luciana Meyer, Wieland Freitas, Dayvison Francis Saraiva Almeida-Paes, Rodrigo |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Coelho, Rowena Alves Brito-Santos, Fábio Figueiredo-Carvalho, Maria Helena Galdino Silva, Juliana Vitoria Dos Santos Gutierrez-Galhardo, Maria Clara Valle, Antonio Carlos Francesconi do Zancopé-Oliveira, Rosely Maria Trilles, Luciana Meyer, Wieland Freitas, Dayvison Francis Saraiva Almeida-Paes, Rodrigo |
| dc.subject.en.pt_BR.fl_str_mv |
Fonsecaea spp. Chromoblastomycosis |
| topic |
Fonsecaea spp. Chromoblastomycosis |
| description |
Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2018 |
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2018 |
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2019-07-11T12:54:37Z |
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2019-07-11T12:54:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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COELHO, Rowena Alves et al. Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil. Plos Neglected Tropical Diseases, p. 1-15, July 2018. |
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https://www.arca.fiocruz.br/handle/icict/33978 |
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1935-2727 |
| dc.identifier.doi.none.fl_str_mv |
10.1371/journal.pntd.0006675 |
| dc.identifier.eissn.none.fl_str_mv |
1935-2735 |
| identifier_str_mv |
COELHO, Rowena Alves et al. Molecular identification and antifungal susceptibility profiles of clinical strains of Fonsecaea spp. isolated from patients with chromoblastomycosis in Rio de Janeiro, Brazil. Plos Neglected Tropical Diseases, p. 1-15, July 2018. 1935-2727 10.1371/journal.pntd.0006675 1935-2735 |
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https://www.arca.fiocruz.br/handle/icict/33978 |
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Public Library of Science |
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Public Library of Science |
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