Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction

Detalhes bibliográficos
Autor(a) principal: Lima, Lyana Rodrigues Pinto
Data de Publicação: 2017
Outros Autores: Silva, Amanda Perse da, Schmidt-Chanasit, Jonas, Paula, Vanessa Salete de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18729
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.
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spelling Lima, Lyana Rodrigues PintoSilva, Amanda Perse daSchmidt-Chanasit, JonasPaula, Vanessa Salete de2017-05-04T12:26:52Z2017-05-04T12:26:52Z2017LIMA, Lyana Rodrigues Pinto; et al. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction. Mem Inst Oswaldo Cruz, v.112, n.3, p.220-223, Mar. 2017.0074-0276https://www.arca.fiocruz.br/handle/icict/1872910.1590/0074-027601603541678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzReação em cadeia da polimerase em tempo realCurva sintéticaHerpesreal time PCRsynthetic curveherpesDiagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reactioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.Bernhard Nocht Institute for Tropical Medicine. Diagnostic Virology Laboratory. Hamburg, Germany.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.The use of quantitative real time polymerase chain reaction (qPCR) for herpesvirus detection has improved the sensitivity and specificity of diagnosis, as it is able to detect shedding episodes in the absence of clinical lesions and diagnose clinical specimens that have low viral loads. With an aim to improve the detection and quantification of herpesvirus by qPCR, synthetic standard curves for human herpesvirus 1 and 2 (HHV-1 and HHV-2) targeting regions gD and gG, respectively, were designed and evaluated. The results show that synthetic curves can replace DNA standard curves in diagnostic herpes qPCR.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18729/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALliana_lima_etal_IOC_2016.pdfliana_lima_etal_IOC_2016.pdfapplication/pdf566527https://www.arca.fiocruz.br/bitstream/icict/18729/2/liana_lima_etal_IOC_2016.pdf98039aceb464c1a4f1c3ede975d17dd3MD52TEXTliana_lima_etal_IOC_2016.pdf.txtliana_lima_etal_IOC_2016.pdf.txtExtracted texttext/plain12160https://www.arca.fiocruz.br/bitstream/icict/18729/3/liana_lima_etal_IOC_2016.pdf.txt09f978a1c0ca7fa08efd8e125ce1f31aMD53icict/187292023-09-14 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dc.title.pt_BR.fl_str_mv Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
title Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
spellingShingle Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
Lima, Lyana Rodrigues Pinto
Reação em cadeia da polimerase em tempo real
Curva sintética
Herpes
real time PCR
synthetic curve
herpes
title_short Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
title_full Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
title_fullStr Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
title_full_unstemmed Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
title_sort Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction
author Lima, Lyana Rodrigues Pinto
author_facet Lima, Lyana Rodrigues Pinto
Silva, Amanda Perse da
Schmidt-Chanasit, Jonas
Paula, Vanessa Salete de
author_role author
author2 Silva, Amanda Perse da
Schmidt-Chanasit, Jonas
Paula, Vanessa Salete de
author2_role author
author
author
dc.contributor.author.fl_str_mv Lima, Lyana Rodrigues Pinto
Silva, Amanda Perse da
Schmidt-Chanasit, Jonas
Paula, Vanessa Salete de
dc.subject.other.pt_BR.fl_str_mv Reação em cadeia da polimerase em tempo real
Curva sintética
Herpes
topic Reação em cadeia da polimerase em tempo real
Curva sintética
Herpes
real time PCR
synthetic curve
herpes
dc.subject.en.pt_BR.fl_str_mv real time PCR
synthetic curve
herpes
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ. Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-05-04T12:26:52Z
dc.date.available.fl_str_mv 2017-05-04T12:26:52Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv LIMA, Lyana Rodrigues Pinto; et al. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction. Mem Inst Oswaldo Cruz, v.112, n.3, p.220-223, Mar. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18729
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160354
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv LIMA, Lyana Rodrigues Pinto; et al. Diagnosis of human herpes virus 1 and 2 (HHV-1 and HHV-2): use of a synthetic standard curve for absolute quantification by real time polymerase chain reaction. Mem Inst Oswaldo Cruz, v.112, n.3, p.220-223, Mar. 2017.
0074-0276
10.1590/0074-02760160354
1678-8060
url https://www.arca.fiocruz.br/handle/icict/18729
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18729/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18729/2/liana_lima_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/18729/3/liana_lima_etal_IOC_2016.pdf.txt
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