LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/25000 |
Resumo: | 481133/2011-9, 483052/2012-4 and 311852/2012-2 from the Brazilian Conselho Nacional de Desenvolvimento CientõÂfico e TecnoloÂgico to AJAM. |
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Conrad, Neida LuciaMcBride, Flavia Weykamp da CruzSouza, Jéssica DiasSilveira, Marcelle MouraFélix, Samuel RodriguesMendonça, Karla SequeiraSantos, Cleiton SilvaAthanazio, Daniel AbensurMedeiros, Marco AlbertoReis, Mitermayer Galvão dosDellagostin, Odir AntonioMcBride, Alan John Alexander2018-02-28T16:27:41Z2018-02-28T16:27:41Z2017CONRAD, N. L. et al. LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. PLOS Neglected Tropical Diseases, v. 11, n. 3, p. e0005441, 2017.1935-2727https://www.arca.fiocruz.br/handle/icict/2500010.1371/journal.pntd.0005441481133/2011-9, 483052/2012-4 and 311852/2012-2 from the Brazilian Conselho Nacional de Desenvolvimento CientõÂfico e TecnoloÂgico to AJAM.Federal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, BrasilCatholic University of Pelotas. Life Science and Health Centre. Pelotas, RGS, BrasilFederal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, BrasilFederal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, BrasilFederal University of Pelotas. Faculty of Veterinary Science. Pelotas, RGS, BrasilFederal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Biomanguinhos. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Medicina da Bahia. Salvador, BA, BrasilFederal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, BrasilFederal University of Pelotas. Technological Development Centre. Biotechnology Unit. Pelotas, RGS, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, BrasilNeglected tropical diseases, including zoonoses such as leptospirosis, have a major impact on rural and poor urban communities, particularly in developing countries. This has led to major investment in antipoverty vaccines that focus on diseases that influence public health and thereby productivity. While the true, global, impact of leptospirosis is unknown due to the lack of adequate laboratory diagnosis, the WHO estimates that incidence has doubled over the last 15 years to over 1 million cases that require hospitalization every year. Leptospirosis is caused by pathogenic Leptospira spp. and is spread through direct contact with infected animals, their urine or contaminated water and soil. Inactivated leptospirosis vaccines, or bacterins, are approved in only a handful of countries due to the lack of heterologous protection (there are > 250 pathogenic Leptospira serovars) and the serious side-effects associated with vaccination. Currently, research has focused on recombinant vaccines, a possible solution to these problems. However, due to a lack of standardised animal models, rigorous statistical analysis and poor reproducibility, this approach has met with limited success. We evaluated a subunit vaccine preparation, based on a conserved region of the leptospiral immunoglobulin-like B protein (LigB(131-645)) and aluminium hydroxide (AH), in the hamster model of leptospirosis. The vaccine conferred significant protection (80.0-100%, P < 0.05) against mortality in vaccinated animals in seven independent experiments. The efficacy of the LigB(131-645)/AH vaccine ranged from 87.5-100% and we observed sterile immunity (87.5-100%) among the vaccinated survivors. Significant levels of IgM and IgG were induced among vaccinated animals, although they did not correlate with immunity. A mixed IgG1/IgG2 subclass profile was associated with the subunit vaccine, compared to the predominant IgG2 profile seen in bacterin vaccinated hamsters. These findings suggest that LigB(131-645) is a vaccine candidate against leptospirosis with potential ramifications to public and veterinary health.engPublic Library of ScienceLeptospiroseLeptospiraVacinaVacinas bacterianasDoenças tropicais negligenciadasAnimaisLeptospirosisLeptospiraVaccinesBacterial VaccinesNeglected tropical diseasesAnimalsLigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/25000/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALConrad NL LigB subunit vaccine confers sterile immunity ....pdfConrad NL LigB subunit vaccine confers sterile immunity 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| dc.title.pt_BR.fl_str_mv |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| title |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| spellingShingle |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis Conrad, Neida Lucia Leptospirose Leptospira Vacina Vacinas bacterianas Doenças tropicais negligenciadas Animais Leptospirosis Leptospira Vaccines Bacterial Vaccines Neglected tropical diseases Animals |
| title_short |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| title_full |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| title_fullStr |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| title_full_unstemmed |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| title_sort |
LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis |
| author |
Conrad, Neida Lucia |
| author_facet |
Conrad, Neida Lucia McBride, Flavia Weykamp da Cruz Souza, Jéssica Dias Silveira, Marcelle Moura Félix, Samuel Rodrigues Mendonça, Karla Sequeira Santos, Cleiton Silva Athanazio, Daniel Abensur Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio McBride, Alan John Alexander |
| author_role |
author |
| author2 |
McBride, Flavia Weykamp da Cruz Souza, Jéssica Dias Silveira, Marcelle Moura Félix, Samuel Rodrigues Mendonça, Karla Sequeira Santos, Cleiton Silva Athanazio, Daniel Abensur Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio McBride, Alan John Alexander |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Conrad, Neida Lucia McBride, Flavia Weykamp da Cruz Souza, Jéssica Dias Silveira, Marcelle Moura Félix, Samuel Rodrigues Mendonça, Karla Sequeira Santos, Cleiton Silva Athanazio, Daniel Abensur Medeiros, Marco Alberto Reis, Mitermayer Galvão dos Dellagostin, Odir Antonio McBride, Alan John Alexander |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospirose Leptospira Vacina Vacinas bacterianas Doenças tropicais negligenciadas Animais |
| topic |
Leptospirose Leptospira Vacina Vacinas bacterianas Doenças tropicais negligenciadas Animais Leptospirosis Leptospira Vaccines Bacterial Vaccines Neglected tropical diseases Animals |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospirosis Leptospira Vaccines Bacterial Vaccines Neglected tropical diseases Animals |
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481133/2011-9, 483052/2012-4 and 311852/2012-2 from the Brazilian Conselho Nacional de Desenvolvimento CientõÂfico e TecnoloÂgico to AJAM. |
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2017 |
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2017 |
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2018-02-28T16:27:41Z |
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2018-02-28T16:27:41Z |
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CONRAD, N. L. et al. LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. PLOS Neglected Tropical Diseases, v. 11, n. 3, p. e0005441, 2017. |
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https://www.arca.fiocruz.br/handle/icict/25000 |
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1935-2727 |
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10.1371/journal.pntd.0005441 |
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CONRAD, N. L. et al. LigB subunit vaccine confers sterile immunity against challenge in the hamster model of leptospirosis. PLOS Neglected Tropical Diseases, v. 11, n. 3, p. e0005441, 2017. 1935-2727 10.1371/journal.pntd.0005441 |
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