Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion

Detalhes bibliográficos
Autor(a) principal: Ramalho-Ortigão, J. M.
Data de Publicação: 2007
Outros Autores: Pitaluga, A. N., Telleria, E. L., Marques, C., Souza, A. A., Traub-Cseko, Y. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44577
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.
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spelling Ramalho-Ortigão, J. M.Pitaluga, A. N.Telleria, E. L.Marques, C.Souza, A. A.Traub-Cseko, Y. M.2020-11-22T19:21:24Z2020-11-22T19:21:24Z2007ORTIGÃO, J. M. Ramalho et al. Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, p. 509-515, June 2007.0074-0276https://www.arca.fiocruz.br/handle/icict/4457710.1590/S0074-027620070004000131678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Lutzomyia longipalpisExpressão diferencialMetaciclogêneseLutzomyia longipalpisV-ATPaseMetacyclogenesisDifferential expressionCloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.Instituto Evandro Chagas. Seção de Parasitologia. Belém, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.Visceral leishmaniasis (VL) is a serious tropical disease that affects approximately 500 thousand people worldwide every year. In the Americas, VL is caused by the parasite Leishmania (Leishmania) infantum chagasi mainly transmitted by the bite of the sand fly vector Lutzomyia longipalpis. Despite recent advances in the study of interaction between Leishmania and sand flies, very little is known about sand fly protein expression profiles. Understanding how the expression of proteins may be affected by blood feeding and/or presence of parasite in the vector's midgut might allow us to devise new strategies for controlling the spread of leishmaniasis. In this work, we report the characterization of a vacuolar ATPase subunit C from L. longipalpis by screening of a midgut cDNA library with a 220 bp fragment identified by means of differential display reverse transcriptase-polymerase chain reaction analysis. The expression of the gene varies along insect development and is upregulated in males and bloodfed L. longipalpis, compared to unfed flies.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44577/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALYM Traub-Cseko_etal_ÎOC_2007.pdfYM Traub-Cseko_etal_ÎOC_2007.pdfapplication/pdf3599015https://www.arca.fiocruz.br/bitstream/icict/44577/2/YM%20Traub-Cseko_etal_%c3%8eOC_2007.pdf75bb53855e000f84c72de3939175a86cMD52TEXTYM Traub-Cseko_etal_ÎOC_2007.pdf.txtYM Traub-Cseko_etal_ÎOC_2007.pdf.txtExtracted texttext/plain30097https://www.arca.fiocruz.br/bitstream/icict/44577/3/YM%20Traub-Cseko_etal_%c3%8eOC_2007.pdf.txt0e6fcf6e2c78cac08a04e37f932d91beMD53icict/445772020-11-23 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dc.title.pt_BR.fl_str_mv Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
title Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
spellingShingle Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
Ramalho-Ortigão, J. M.
Lutzomyia longipalpis
Expressão diferencial
Metaciclogênese
Lutzomyia longipalpis
V-ATPase
Metacyclogenesis
Differential expression
title_short Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
title_full Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
title_fullStr Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
title_full_unstemmed Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
title_sort Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion
author Ramalho-Ortigão, J. M.
author_facet Ramalho-Ortigão, J. M.
Pitaluga, A. N.
Telleria, E. L.
Marques, C.
Souza, A. A.
Traub-Cseko, Y. M.
author_role author
author2 Pitaluga, A. N.
Telleria, E. L.
Marques, C.
Souza, A. A.
Traub-Cseko, Y. M.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ramalho-Ortigão, J. M.
Pitaluga, A. N.
Telleria, E. L.
Marques, C.
Souza, A. A.
Traub-Cseko, Y. M.
dc.subject.other.pt_BR.fl_str_mv Lutzomyia longipalpis
Expressão diferencial
Metaciclogênese
topic Lutzomyia longipalpis
Expressão diferencial
Metaciclogênese
Lutzomyia longipalpis
V-ATPase
Metacyclogenesis
Differential expression
dc.subject.en.pt_BR.fl_str_mv Lutzomyia longipalpis
V-ATPase
Metacyclogenesis
Differential expression
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Laboratório de Biologia Molecular de Tripanossomatídios e Flebotomíneos. Rio de Janeiro, RJ, Brasil.
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2020-11-22T19:21:24Z
dc.date.available.fl_str_mv 2020-11-22T19:21:24Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ORTIGÃO, J. M. Ramalho et al. Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, p. 509-515, June 2007.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44577
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762007000400013
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ORTIGÃO, J. M. Ramalho et al. Cloning and characterization of a V-ATPase subunit C from the American visceral leishmaniasis vector Lutzomyia longipalpis modulated during development and blood ingestion. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 4, p. 509-515, June 2007.
0074-0276
10.1590/S0074-02762007000400013
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44577
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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