Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1371/journal.pone.0037283 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19012 |
Resumo: | Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil. |
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Garza, Daniel RiosThompson, Cristiane C.Loureiro, Edvaldo Carlos BritoDutilh, Bas E.Inada, Davi ToshioSousa Junior, Edivaldo CostaCardoso, Jedson FerreiraNunes, Márcio Roberto T.Lima, Clayton Pereira Silva deSilvestre, Rodrigo Vellasco DuarteNunes, Keley Nascimento BarbosaSantos, Elisabeth C. O.Edwards, Robert A.Vicente, Ana Carolina P.Morais, Lena Lillian Canto de Sá2017-05-25T18:09:28Z2017-05-25T18:09:28Z2012GARZA, Daniel Rios; et al. Genome-Wide Study of the Defective Sucrose Fermenter Strain of Vibrio cholerae from the Latin American Cholera Epidemic. Plos One, v.7, n.5, e37283, May 2012.1932-6203https://www.arca.fiocruz.br/handle/icict/1901210.1371/journal.pone.0037283engPublic Library of ScienceEpidemia de cóleraDeficiência de Fermentador de SacaroseAmérica LatinaVibrio choleraeLatin AmericanCholera EpidemicDefective Sucrose FermenterGenome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Instituto Evandro Chagas. Secção de Bacteriologia. Ananindeua, PA, Brasil.Radboud University. Nijmegen Medical Centre. Centre for Molecular and Biomolecular Informatics. Nijmegen, The Netherlands / Radboud University. Nijmegen Medical Centre. Centre for Molecular Life Sciences. Nijmegen, The Netherlands / San Diego State University. Department of Computer Scoence. San Diego, CA, USA / San Diego State University. Department of Biology. San Diego, CA, USA.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Centro de Inovação tecnológica. Ananindeua, PA, Brasil.Instituto Evandro Chagas. Ananindeua, PA, Brasil.San Diego State University. Department of Computer Science. San Diego, CA, USA / Argonne National Laboratory. Mathematics and Computer Science Division. Argonne, Illinois, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microorganismos. Rio de Janeiro, RJ. Brasil.Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil.The 7th cholera pandemic reached Latin America in 1991, spreading from Peru to virtually all Latin American countries. During the late epidemic period, a strain that failed to ferment sucrose dominated cholera outbreaks in the Northern Brazilian Amazon region. In order to understand the genomic characteristics and the determinants of this altered sucrose fermenting phenotype, the genome of the strain IEC224 was sequenced. This paper reports a broad genomic study of this strain, showing its correlation with the major epidemic lineage. The potentially mobile genomic regions are shown to possess GC content deviation, and harbor the main V. cholera virulence genes. A novel bioinformatic approach was applied in order to identify the putative functions of hypothetical proteins, and was compared with the automatic annotation by RAST. The genome of a large bacteriophage was found to be integrated to the IEC224's alanine aminopeptidase gene. The presence of this phage is shown to be a common characteristic of the El Tor strains from the Latin American epidemic, as well as its putative ancestor from Angola. The defective sucrose fermenting phenotype is shown to be due to a single nucleotide insertion in the V. cholerae sucrose-specific transportation gene. This frame-shift mutation truncated a membrane protein, altering its structural pore-like conformation. Further, the identification of a common bacteriophage reinforces both the monophyletic and African-Origin hypotheses for the main causative agent of the 1991 Latin America cholera epidemics.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19012/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALcristiane_thompson_etal_IOC_2012.pdfcristiane_thompson_etal_IOC_2012.pdfapplication/pdf3374042https://www.arca.fiocruz.br/bitstream/icict/19012/2/cristiane_thompson_etal_IOC_2012.pdfe677d27c5d16011d3b21f330a06a8fbcMD52TEXTcristiane_thompson_etal_IOC_2012.pdf.txtcristiane_thompson_etal_IOC_2012.pdf.txtExtracted texttext/plain45946https://www.arca.fiocruz.br/bitstream/icict/19012/3/cristiane_thompson_etal_IOC_2012.pdf.txt715db925cf1387cbf372d4340f7eecf0MD53icict/190122018-08-15 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| dc.title.pt_BR.fl_str_mv |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| title |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| spellingShingle |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic Garza, Daniel Rios Epidemia de cólera Deficiência de Fermentador de Sacarose América Latina Vibrio cholerae Latin American Cholera Epidemic Defective Sucrose Fermenter |
| title_short |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| title_full |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| title_fullStr |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| title_full_unstemmed |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| title_sort |
Genome-wide study of the defective sucrose fermenter strain of Vibrio cholerae from the Latin American cholera epidemic |
| author |
Garza, Daniel Rios |
| author_facet |
Garza, Daniel Rios Thompson, Cristiane C. Loureiro, Edvaldo Carlos Brito Dutilh, Bas E. Inada, Davi Toshio Sousa Junior, Edivaldo Costa Cardoso, Jedson Ferreira Nunes, Márcio Roberto T. Lima, Clayton Pereira Silva de Silvestre, Rodrigo Vellasco Duarte Nunes, Keley Nascimento Barbosa Santos, Elisabeth C. O. Edwards, Robert A. Vicente, Ana Carolina P. Morais, Lena Lillian Canto de Sá |
| author_role |
author |
| author2 |
Thompson, Cristiane C. Loureiro, Edvaldo Carlos Brito Dutilh, Bas E. Inada, Davi Toshio Sousa Junior, Edivaldo Costa Cardoso, Jedson Ferreira Nunes, Márcio Roberto T. Lima, Clayton Pereira Silva de Silvestre, Rodrigo Vellasco Duarte Nunes, Keley Nascimento Barbosa Santos, Elisabeth C. O. Edwards, Robert A. Vicente, Ana Carolina P. Morais, Lena Lillian Canto de Sá |
| author2_role |
author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Garza, Daniel Rios Thompson, Cristiane C. Loureiro, Edvaldo Carlos Brito Dutilh, Bas E. Inada, Davi Toshio Sousa Junior, Edivaldo Costa Cardoso, Jedson Ferreira Nunes, Márcio Roberto T. Lima, Clayton Pereira Silva de Silvestre, Rodrigo Vellasco Duarte Nunes, Keley Nascimento Barbosa Santos, Elisabeth C. O. Edwards, Robert A. Vicente, Ana Carolina P. Morais, Lena Lillian Canto de Sá |
| dc.subject.other.pt_BR.fl_str_mv |
Epidemia de cólera Deficiência de Fermentador de Sacarose América Latina |
| topic |
Epidemia de cólera Deficiência de Fermentador de Sacarose América Latina Vibrio cholerae Latin American Cholera Epidemic Defective Sucrose Fermenter |
| dc.subject.en.pt_BR.fl_str_mv |
Vibrio cholerae Latin American Cholera Epidemic Defective Sucrose Fermenter |
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Instituto Evandro Chagas. Laboratório de Microbiologia Ambiental. Ananindeua, PA, Brasil. |
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2012 |
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2012 |
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2017-05-25T18:09:28Z |
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2017-05-25T18:09:28Z |
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GARZA, Daniel Rios; et al. Genome-Wide Study of the Defective Sucrose Fermenter Strain of Vibrio cholerae from the Latin American Cholera Epidemic. Plos One, v.7, n.5, e37283, May 2012. |
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https://www.arca.fiocruz.br/handle/icict/19012 |
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1932-6203 |
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10.1371/journal.pone.0037283 |
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GARZA, Daniel Rios; et al. Genome-Wide Study of the Defective Sucrose Fermenter Strain of Vibrio cholerae from the Latin American Cholera Epidemic. Plos One, v.7, n.5, e37283, May 2012. 1932-6203 10.1371/journal.pone.0037283 |
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