Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi

Detalhes bibliográficos
Autor(a) principal: Ferreira, Ana Gisele da Costa Neves
Data de Publicação: 2004
Outros Autores: Andrade, Carlos Mauricio de, Santos, Marcos André Vannier dos, Perales, Jonas Enrique Aquiles, Nascimento, Hilton Jorge, Silva Junior, José Godinho da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19297
Resumo: CNPq, FAPERJ, and PAPES-Fiocruz.
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spelling Ferreira, Ana Gisele da Costa NevesAndrade, Carlos Mauricio deSantos, Marcos André Vannier dosPerales, Jonas Enrique AquilesNascimento, Hilton JorgeSilva Junior, José Godinho da2017-06-05T13:26:26Z2017-06-05T13:26:26Z2004FERREIRA, A. G. C. N. et al. Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi. The Protein Journal, v. 23, n. 1, p. 71-77, 2004.0277-8033https://www.arca.fiocruz.br/handle/icict/19297CNPq, FAPERJ, and PAPES-Fiocruz.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Ciências Biológicas. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Microscopia Eletrônica. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, BrasilUniversidade Federal do Rio de Janeiro. Instituto de Química. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil / Universidade Federal Rural. Instituto de Ciências Exatas. Departamento de Química. Rio de Janeiro, RJ, Brasil /Omp-28 isolated from Salmonella enterica serovar typhi presented a subunit molecular mass of 9,632 Da by MALDI-TOF MS. It was denatured, S-alkylated, and 1) directly submitted to Edman sequencing, 2) cleaved with CNBr, and 3) hydrolyzed either with endoproteinase Glu-C or Asp-N. The major CNBr peptide containing the C-terminal portion of Omp-28 was isolated by tricine-SDS-PAGE and electroblotted whereas Omp-28 enzymatic peptides were isolated by C18-RP-HPLC. All peptides were sequenced. This approach allowed the elucidation of the complete primary structure of Omp-28. Its amino acid sequence is identical to that deduced from part of the DNA of the “putative periplasmic transport protein” of either S. enterica serovar typhimurium and a multiple drug resistant S. enterica serovar typhi. Omp-28 homologous protein sequences were also deduced from Escherichia coli and Yersinia pestis genomic DNA. All proteins had their secondary structures predicted. Immunogold cytochemistry indicated that Omp-28 is found on the bacterium outer membrane.engPlenum Publishing CorporationSequência de aminoácidosOmp-28Proteína da membrana externaSalmonella entericaSerovar typhiAmino acid sequenceOmp-28Outer membrane proteinSalmonella entericaSerovar typhiSequência de aminoácidosSalmonella entericaComplete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19297/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFerreira AGCN Complete amino acid....pdfFerreira AGCN Complete amino 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dc.title.pt_BR.fl_str_mv Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
title Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
spellingShingle Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
Ferreira, Ana Gisele da Costa Neves
Sequência de aminoácidos
Omp-28
Proteína da membrana externa
Salmonella enterica
Serovar typhi
Amino acid sequence
Omp-28
Outer membrane protein
Salmonella enterica
Serovar typhi
Sequência de aminoácidos
Salmonella enterica
title_short Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
title_full Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
title_fullStr Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
title_full_unstemmed Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
title_sort Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi
author Ferreira, Ana Gisele da Costa Neves
author_facet Ferreira, Ana Gisele da Costa Neves
Andrade, Carlos Mauricio de
Santos, Marcos André Vannier dos
Perales, Jonas Enrique Aquiles
Nascimento, Hilton Jorge
Silva Junior, José Godinho da
author_role author
author2 Andrade, Carlos Mauricio de
Santos, Marcos André Vannier dos
Perales, Jonas Enrique Aquiles
Nascimento, Hilton Jorge
Silva Junior, José Godinho da
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Ferreira, Ana Gisele da Costa Neves
Andrade, Carlos Mauricio de
Santos, Marcos André Vannier dos
Perales, Jonas Enrique Aquiles
Nascimento, Hilton Jorge
Silva Junior, José Godinho da
dc.subject.other.pt_BR.fl_str_mv Sequência de aminoácidos
Omp-28
Proteína da membrana externa
Salmonella enterica
Serovar typhi
topic Sequência de aminoácidos
Omp-28
Proteína da membrana externa
Salmonella enterica
Serovar typhi
Amino acid sequence
Omp-28
Outer membrane protein
Salmonella enterica
Serovar typhi
Sequência de aminoácidos
Salmonella enterica
dc.subject.en.pt_BR.fl_str_mv Amino acid sequence
Omp-28
Outer membrane protein
Salmonella enterica
Serovar typhi
dc.subject.decs.pt_BR.fl_str_mv Sequência de aminoácidos
Salmonella enterica
description CNPq, FAPERJ, and PAPES-Fiocruz.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2017-06-05T13:26:26Z
dc.date.available.fl_str_mv 2017-06-05T13:26:26Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FERREIRA, A. G. C. N. et al. Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi. The Protein Journal, v. 23, n. 1, p. 71-77, 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19297
dc.identifier.issn.pt_BR.fl_str_mv 0277-8033
identifier_str_mv FERREIRA, A. G. C. N. et al. Complete amino acid sequence and location of Omp-28, an important immunogenic protein from Salmonella enterica serovar typhi. The Protein Journal, v. 23, n. 1, p. 71-77, 2004.
0277-8033
url https://www.arca.fiocruz.br/handle/icict/19297
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Plenum Publishing Corporation
publisher.none.fl_str_mv Plenum Publishing Corporation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/19297/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/19297/2/Ferreira%20AGCN%20Complete%20amino%20acid....pdf
https://www.arca.fiocruz.br/bitstream/icict/19297/3/Ferreira%20AGCN%20Complete%20amino%20acid....pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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