Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47682 |
Resumo: | Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. |
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Medeiros, Fernanda Alvarenga CardosoSouza Filho, Job Alves deBarbosa, José RonaldoDonato, Lucas EdelFigueiredo, Fabiano BorgesWerneck, Guilherme LoureiroPaz, Gustavo FontesThompson, MayersonMarcelino, Andreza Pain2021-06-14T20:42:43Z2021-06-14T20:42:43Z2021MEDEIROS, Fernanda Alvarenga Cardoso et al. Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil. Cad. Saúde Pública, v. 37, n. 3, p. 1–11, 2021.1678-4464https://www.arca.fiocruz.br/handle/icict/4768210.1590/0102-311X00041320porFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Brasil.Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Ministério da Saúde. Brasília, DF, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade do Estado do Rio de Janeiro. Instituto de Medicina Social. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto René Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Dogs are the main reservoirs in the domestic transmission cycle of visceral leishmaniasis, and the diagnosis is essential for the effectiveness of the control measures recommended by the Brazilian Ministry of Health. We assessed the diagnostic performance of the ELISA-Vetlisa/BIOCLIN prototype with serum samples from 200 dogs, in triplicate, including symptomatic, oligosymptomatic, asymptomatic, and healthy dogs, originated by two distinct panels (A and B) characterized by parasitological tests as the reference standard. In this study, the prototype kit showed a 99% sensitivity (95%CI: 94.5-100.0) and a 100% specificity (95%CI: 96.4-100.0). The sensitivity of the prototype kit did not vary significantly with the clinical status of the dogs. Considering the final result classification (positive or negative), agreement between the results of repeated tests was almost perfect (kappa = 0.99; 95%CI: 0.98-1.00). ELISAVetlisa/BIOCLIN is a promising option for the serological diagnosis of canine visceral leishmaniasis in Brazil.Enzyme-Linked Immunosorbent AssayLeishmaniasis, VisceralDogsDiagnosisEnsayo de Inmunoadsorción EnzimáticaLeishmaniasis VisceralPerrosTest ELISALeishmaniose viscéraleChiensDiagnosticEnsaio de Imunoadsorção EnzimáticaLeishmaniose VisceralCãesDiagnósticoinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/47682/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALPhaseII-ValidationStudyOK.pdfPhaseII-ValidationStudyOK.pdfapplication/pdf305241https://www.arca.fiocruz.br/bitstream/icict/47682/2/PhaseII-ValidationStudyOK.pdf8c74171fb8d1465ce2ecdf41013bc2c7MD52TEXTPhaseII-ValidationStudyOK.pdf.txtPhaseII-ValidationStudyOK.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| title |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| spellingShingle |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil Medeiros, Fernanda Alvarenga Cardoso Enzyme-Linked Immunosorbent Assay Leishmaniasis, Visceral Dogs Diagnosis Ensayo de Inmunoadsorción Enzimática Leishmaniasis Visceral Perros Test ELISA Leishmaniose viscérale Chiens Diagnostic Ensaio de Imunoadsorção Enzimática Leishmaniose Visceral Cães Diagnóstico |
| title_short |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| title_full |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| title_fullStr |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| title_full_unstemmed |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| title_sort |
Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil |
| author |
Medeiros, Fernanda Alvarenga Cardoso |
| author_facet |
Medeiros, Fernanda Alvarenga Cardoso Souza Filho, Job Alves de Barbosa, José Ronaldo Donato, Lucas Edel Figueiredo, Fabiano Borges Werneck, Guilherme Loureiro Paz, Gustavo Fontes Thompson, Mayerson Marcelino, Andreza Pain |
| author_role |
author |
| author2 |
Souza Filho, Job Alves de Barbosa, José Ronaldo Donato, Lucas Edel Figueiredo, Fabiano Borges Werneck, Guilherme Loureiro Paz, Gustavo Fontes Thompson, Mayerson Marcelino, Andreza Pain |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Medeiros, Fernanda Alvarenga Cardoso Souza Filho, Job Alves de Barbosa, José Ronaldo Donato, Lucas Edel Figueiredo, Fabiano Borges Werneck, Guilherme Loureiro Paz, Gustavo Fontes Thompson, Mayerson Marcelino, Andreza Pain |
| dc.subject.en.pt_BR.fl_str_mv |
Enzyme-Linked Immunosorbent Assay Leishmaniasis, Visceral Dogs Diagnosis |
| topic |
Enzyme-Linked Immunosorbent Assay Leishmaniasis, Visceral Dogs Diagnosis Ensayo de Inmunoadsorción Enzimática Leishmaniasis Visceral Perros Test ELISA Leishmaniose viscérale Chiens Diagnostic Ensaio de Imunoadsorção Enzimática Leishmaniose Visceral Cães Diagnóstico |
| dc.subject.es.pt_BR.fl_str_mv |
Ensayo de Inmunoadsorción Enzimática Leishmaniasis Visceral Perros |
| dc.subject.fr.pt_BR.fl_str_mv |
Test ELISA Leishmaniose viscérale Chiens Diagnostic |
| dc.subject.decs.pt_BR.fl_str_mv |
Ensaio de Imunoadsorção Enzimática Leishmaniose Visceral Cães Diagnóstico |
| description |
Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil. |
| publishDate |
2021 |
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2021-06-14T20:42:43Z |
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2021-06-14T20:42:43Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MEDEIROS, Fernanda Alvarenga Cardoso et al. Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil. Cad. Saúde Pública, v. 37, n. 3, p. 1–11, 2021. |
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https://www.arca.fiocruz.br/handle/icict/47682 |
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1678-4464 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0102-311X00041320 |
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MEDEIROS, Fernanda Alvarenga Cardoso et al. Phase II validation study of the rK39 ELISA prototype for the diagnosis of canine visceral leishmaniasis in Brazil. Cad. Saúde Pública, v. 37, n. 3, p. 1–11, 2021. 1678-4464 10.1590/0102-311X00041320 |
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https://www.arca.fiocruz.br/handle/icict/47682 |
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Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Brasil. |
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Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Brasil. |
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