Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2015 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/12055 |
Resumo: | 2016-03-31 |
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Pereira, Polyana SilvaBorghi, MirlaAlbano, Rodolpho MattosLopes, Jonathan Christian OliveiraSilveira, Melise ChavesMarques, Elizabeth AndradeOliveira, Jane Cleide RibeiroAsensi, Marise DutraAssef, Ana Paula D’Alincourt Carvalho2015-10-22T16:39:08Z2016-04-01T07:30:08Z2015PEREIRA, Polyana Silva; et al. Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil. Microbial Drug Resistance, v.21, n.2, p.234-236, Apr. 2015.1931-8448https://www.arca.fiocruz.br/handle/icict/1205510.1089/mdr.2014.0171engMary Ann Liebert, Inc. publishersCoproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2016-03-31Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ). Instituto de Biologia Roberto Alcântara Gomes. Departamento de Bioquímica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro (UERJ). Faculdade de Ciências Médicas. Departamento de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil.Laboratório Central de Saúde Pública do Rio de Janeiro Noel Nutels (LACEN/RJ). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar (LAPIH). Rio de Janeiro, RJ, Brasil.The most important resistance mechanism against b-lactam drugs is the production of carbapenemases. In this study, we report the first identification of Klebsiella pneumoniae carbapenemase (KPC)-2 and New Delhi metallo-b-lactamase (NDM)-1 in Enterobacter hormaechei subps. oharae from Brazil. The detection of carbapenemases was done by phenotypic assays, PCR, and DNA sequencing, whereas the identification was performed by conventional techniques, sequencing of the 16S rDNA gene, and hsp60-genotyping. Molecular typing was performed using pulsed-field gel electrophoresis, and antimicrobial susceptibility was surrogated by the Etest methodology. Using the whole genome sequencing approach, we searched for resistance genes, plasmid incompatibility group genes, and the genetic environment of blaNDM and blaKPC. The plasmid identification was done by restriction digests with the S1 nuclease followed by hybridization using digoxigenin labeled specific probes. The isolate was considered multiresistant, being susceptible to amikacin and polymyxin B. We observed the following resistance genes: blaCTX-M-15, blaACT-7, blaTEM-1, blaOXA-1, aadA1, aadA2, strA, strB, aac(3)-II, qnrB1, and aac(6¢)-Ib-cr and incompatibility group plasmid genes IncA/C, IncHI2, and IncN. The blaKPC gene was found associated to the transposon Tn4401 isoform b in plasmid with 50 kb (IncN) and blaNDM-1 was flanked by a truncated ISAba125 and bleMBL in plasmid with 160 kb (IncA/C). This study showed the coproduction of two important carbapenemases (KPC-2 and NDM-1) associated with mobile genetic elements of worldwide epidemiological importance (Tn4401 and ISAba125, respectively), reinforcing the idea that urgent measures are necessary to reduce and prevent the spreading of these carbapenemases primarily in the hospital settings.Enterobacter hormaecheiKPC-2Klebsiella pneumoniaeBrazilNDM-1EnterobacterKlebsiella pneumoniaeBrasilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82354https://www.arca.fiocruz.br/bitstream/icict/12055/1/license.txt8b4c200b4e10021c5683c6ccaba07169MD51ORIGINALpolyana_pereira_etal_IOC-2015.pdfpolyana_pereira_etal_IOC-2015.pdfapplication/pdf85386https://www.arca.fiocruz.br/bitstream/icict/12055/2/polyana_pereira_etal_IOC-2015.pdf7a1dee5713ed206b701d30e920869cc5MD52TEXTpolyana_pereira_etal_IOC-2015.pdf.txtpolyana_pereira_etal_IOC-2015.pdf.txtExtracted texttext/plain14804https://www.arca.fiocruz.br/bitstream/icict/12055/3/polyana_pereira_etal_IOC-2015.pdf.txtbef9cc55872057eb111e5b20da8833b1MD53icict/120552023-09-05 10:30:54.381oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-05T13:30:54Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| title |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| spellingShingle |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil Pereira, Polyana Silva Enterobacter hormaechei KPC-2 Klebsiella pneumoniae Brazil NDM-1 Enterobacter Klebsiella pneumoniae Brasil |
| title_short |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| title_full |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| title_fullStr |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| title_full_unstemmed |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| title_sort |
Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil |
| author |
Pereira, Polyana Silva |
| author_facet |
Pereira, Polyana Silva Borghi, Mirla Albano, Rodolpho Mattos Lopes, Jonathan Christian Oliveira Silveira, Melise Chaves Marques, Elizabeth Andrade Oliveira, Jane Cleide Ribeiro Asensi, Marise Dutra Assef, Ana Paula D’Alincourt Carvalho |
| author_role |
author |
| author2 |
Borghi, Mirla Albano, Rodolpho Mattos Lopes, Jonathan Christian Oliveira Silveira, Melise Chaves Marques, Elizabeth Andrade Oliveira, Jane Cleide Ribeiro Asensi, Marise Dutra Assef, Ana Paula D’Alincourt Carvalho |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pereira, Polyana Silva Borghi, Mirla Albano, Rodolpho Mattos Lopes, Jonathan Christian Oliveira Silveira, Melise Chaves Marques, Elizabeth Andrade Oliveira, Jane Cleide Ribeiro Asensi, Marise Dutra Assef, Ana Paula D’Alincourt Carvalho |
| dc.subject.en.pt_BR.fl_str_mv |
Enterobacter hormaechei KPC-2 Klebsiella pneumoniae Brazil NDM-1 |
| topic |
Enterobacter hormaechei KPC-2 Klebsiella pneumoniae Brazil NDM-1 Enterobacter Klebsiella pneumoniae Brasil |
| dc.subject.decs.pt_BR.fl_str_mv |
Enterobacter Klebsiella pneumoniae Brasil |
| description |
2016-03-31 |
| publishDate |
2015 |
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2015-10-22T16:39:08Z |
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2015 |
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2016-04-01T07:30:08Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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PEREIRA, Polyana Silva; et al. Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil. Microbial Drug Resistance, v.21, n.2, p.234-236, Apr. 2015. |
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https://www.arca.fiocruz.br/handle/icict/12055 |
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1931-8448 |
| dc.identifier.doi.none.fl_str_mv |
10.1089/mdr.2014.0171 |
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PEREIRA, Polyana Silva; et al. Coproduction of NDM-1 and KPC-2 in Enterobacter hormaechei from Brazil. Microbial Drug Resistance, v.21, n.2, p.234-236, Apr. 2015. 1931-8448 10.1089/mdr.2014.0171 |
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info:eu-repo/semantics/openAccess |
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Mary Ann Liebert, Inc. publishers |
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Mary Ann Liebert, Inc. publishers |
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