New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis

Detalhes bibliográficos
Autor(a) principal: Boité, Mariana C.
Data de Publicação: 2012
Outros Autores: Mauricio, Isabel L., Miles, Michael A., Cupolillo, Elisa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18490
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.
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spelling Boité, Mariana C.Mauricio, Isabel L.Miles, Michael A.Cupolillo, Elisa2017-04-18T14:45:52Z2017-04-18T14:45:52Z2012BOITÉ, Mariana C. et al. New Insights on Taxonomy, Phylogeny and Population Genetics of Leishmania (Viannia) Parasites Based on Multilocus Sequence Analysis. PLoS Negl Trop Dis, v.6, n.11, e1888. 14p, Nov. 2012.1935-2727https://www.arca.fiocruz.br/handle/icict/1849010.1371/journal.pntd.00018881935-2735engPublic Library of ScienceTaxonomiaLeishmaniaParasitosFilogeniaGenética populacionalLeishmania (Viannia)ParasitesTaxonomyPhylogenyPopulation GeneticsNew insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. Department of Pathogen Molecular Biology. London, UK / Instituto de Higiene e Medicina Tropical. Unidade de Parasitologia e Microbiologia Médicas. Lisboa, Portugal.London School of Hygiene and Tropical Medicine. Faculty of Infectious and Tropical Diseases. Department of Pathogen Molecular Biology. London, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.The Leishmania genus comprises up to 35 species, some with status still under discussion. The multilocus sequence typing (MLST)--extensively used for bacteria--has been proposed for pathogenic trypanosomatids. For Leishmania, however, a detailed analysis and revision on the taxonomy is still required. We have partially sequenced four housekeeping genes--glucose-6-phosphate dehydrogenase (G6PD), 6-phosphogluconate dehydrogenase (6PGD), mannose phosphate isomerase (MPI) and isocitrate dehydrogenase (ICD)--from 96 Leishmania (Viannia) strains and assessed their discriminatory typing capacity. The fragments had different degrees of diversity, and are thus suitable to be used in combination for intra- and inter-specific inferences. Species-specific single nucleotide polymorphisms were detected, but not for all species; ambiguous sites indicating heterozygosis were observed, as well as the putative homozygous donor. A large number of haplotypes were detected for each marker; for 6PGD a possible ancestral allele for L. (Viannia) was found. Maximum parsimony-based haplotype networks were built. Strains of different species, as identified by multilocus enzyme electrophoresis (MLEE), formed separated clusters in each network, with exceptions. NeighborNet of concatenated sequences confirmed species-specific clusters, suggesting recombination occurring in L. braziliensis and L. guyanensis. Phylogenetic analysis indicates L. lainsoni and L. naiffi as the most divergent species and does not support L. shawi as a distinct species, placing it in the L. guyanensis cluster. BURST analysis resulted in six clonal complexes (CC), corresponding to distinct species. The L. braziliensis strains evaluated correspond to one widely geographically distributed CC and another restricted to one endemic area. This study demonstrates the value of systematic multilocus sequence analysis (MLSA) for determining intra- and inter-species relationships and presents an approach to validate the species status of some entities. Furthermore, it contributes to the phylogeny of L. (Viannia) and might be helpful for epidemiological and population genetics analysis based on haplotype/diplotype determinations and inferences.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/18490/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALelisa_cupolillo_etal_IOC_2012.pdfelisa_cupolillo_etal_IOC_2012.pdfapplication/pdf895287https://www.arca.fiocruz.br/bitstream/icict/18490/2/elisa_cupolillo_etal_IOC_2012.pdf99e39a5e52ad7b4be73823bff1cc9cd3MD52TEXTelisa_cupolillo_etal_IOC_2012.pdf.txtelisa_cupolillo_etal_IOC_2012.pdf.txtExtracted texttext/plain66744https://www.arca.fiocruz.br/bitstream/icict/18490/3/elisa_cupolillo_etal_IOC_2012.pdf.txtc6ffd6455b26c5c269630a6695bd2e20MD53icict/184902018-08-15 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dc.title.pt_BR.fl_str_mv New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
title New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
spellingShingle New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
Boité, Mariana C.
Taxonomia
Leishmania
Parasitos
Filogenia
Genética populacional
Leishmania (Viannia)
Parasites
Taxonomy
Phylogeny
Population Genetics
title_short New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
title_full New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
title_fullStr New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
title_full_unstemmed New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
title_sort New insights on taxonomy, phylogeny and population genetics of Leishmania (Viannia) parasites based on multilocus sequence analysis
author Boité, Mariana C.
author_facet Boité, Mariana C.
Mauricio, Isabel L.
Miles, Michael A.
Cupolillo, Elisa
author_role author
author2 Mauricio, Isabel L.
Miles, Michael A.
Cupolillo, Elisa
author2_role author
author
author
dc.contributor.author.fl_str_mv Boité, Mariana C.
Mauricio, Isabel L.
Miles, Michael A.
Cupolillo, Elisa
dc.subject.other.pt_BR.fl_str_mv Taxonomia
Leishmania
Parasitos
Filogenia
Genética populacional
topic Taxonomia
Leishmania
Parasitos
Filogenia
Genética populacional
Leishmania (Viannia)
Parasites
Taxonomy
Phylogeny
Population Genetics
dc.subject.en.pt_BR.fl_str_mv Leishmania (Viannia)
Parasites
Taxonomy
Phylogeny
Population Genetics
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ. Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2017-04-18T14:45:52Z
dc.date.available.fl_str_mv 2017-04-18T14:45:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BOITÉ, Mariana C. et al. New Insights on Taxonomy, Phylogeny and Population Genetics of Leishmania (Viannia) Parasites Based on Multilocus Sequence Analysis. PLoS Negl Trop Dis, v.6, n.11, e1888. 14p, Nov. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18490
dc.identifier.issn.pt_BR.fl_str_mv 1935-2727
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001888
dc.identifier.eissn.none.fl_str_mv 1935-2735
identifier_str_mv BOITÉ, Mariana C. et al. New Insights on Taxonomy, Phylogeny and Population Genetics of Leishmania (Viannia) Parasites Based on Multilocus Sequence Analysis. PLoS Negl Trop Dis, v.6, n.11, e1888. 14p, Nov. 2012.
1935-2727
10.1371/journal.pntd.0001888
1935-2735
url https://www.arca.fiocruz.br/handle/icict/18490
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/18490/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/18490/2/elisa_cupolillo_etal_IOC_2012.pdf
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