Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/38700 |
Resumo: | Instituto Butantan. Laboratório de Bioquímica e Biofísica. São Paulo, SP, Brasil. |
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Modesto, Jeanne Claíne de AlbuquerqueAzevedo, Inácio Loióla Meireles Junqueira deFerreira, Ana Gisele C. NevesFritzen, MárcioOliva, Maria Luíza VilelaHo, Paulo LeePerales, JonasChudzinski-Tavassi, Ana Marisa2019-12-19T12:24:49Z2019-12-19T12:24:49Z2005MODESTO, Jeanne Claine de Albuquerque et al. Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains. Biological Chemistry, v. 386, p. 589-600, June 2005.1431-6730https://www.arca.fiocruz.br/handle/icict/3870010.1515/BC.2005.0691437-4315engDe GruyterBothrops insularisCoagulaçãoEndothelial cellMetaloproteaseAtivador de protrombinaVeneno de cobraBothrops insularisCoagulationEndothelial cellProthrombin activatorSnake venomMetalloproteaseInsularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domainsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan. Laboratório de Bioquímica e Biofísica. São Paulo, SP, Brasil.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Laboratório de Bioquímica e Biofísica. São Paulo, SP, Brasil.Universidade Federal de São Paulo. Departamento de Bioquímica. São Paulo, SP, Brasil.Instituto Butantan. Centro de Biotecnologia. São Paulo, SP, Brasil / Universidade de São Paulo. Instituto de Biociências. Instituto de Química. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Fisiologia e Farmacodinâmica. Rio de Janeiro, RJ, Brasil.Instituto Butantan. Laboratório de Bioquímica e Biofísica. São Paulo, SP, Brasil.The first low-molecular-mass metalloprotease presenting prothrombin activating activity was purified from Bothrops insularis venom and named insularinase A. It is a single-chain protease with a molecular mass of 22 639 Da. cDNA sequence analysis revealed that the disintegrin domain of the precursor protein is post-translationally processed, producing the mature insularinase A. Analysis of its deduced amino acid sequence showed a high similarity with several fibrin(ogen)olytic metalloproteases and only a moderate similarity with prothrombin activators. However, SDS-PAGE of prothrombin after activation by insularinase A showed fragment patterns similar to those generated by group A prothrombin activators, which convert prothrombin into meizothrombin independently of the prothrombinase complex. In addition, insularinase A activates factor X and hydrolyses fibrinogen and fibrin. Chelating agents fully inhibit all insularinase A activities. Insularinase A induced neither detachment nor apoptosis of human endothelial cells and was also not able to trigger an endothelial proinflammatory cell response. Nitric oxide and prostacyclin levels released by endothelial cells were significantly increased after treatment with insularinase A. Our results show that, although its primary structure is related to class P-I fibrin(ogen)olytic metalloproteases, insularinase A is functionally similar to group A prothrombin activators.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/38700/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALANAGiseleFereira_JonasPerales_etal_IOC_2005.pdfANAGiseleFereira_JonasPerales_etal_IOC_2005.pdfapplication/pdf546005https://www.arca.fiocruz.br/bitstream/icict/38700/2/ANAGiseleFereira_JonasPerales_etal_IOC_2005.pdfe24f0c5d3520428ad449455ed035164fMD52TEXTANAGiseleFereira_JonasPerales_etal_IOC_2005.pdf.txtANAGiseleFereira_JonasPerales_etal_IOC_2005.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| title |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| spellingShingle |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains Modesto, Jeanne Claíne de Albuquerque Bothrops insularis Coagulação Endothelial cell Metaloprotease Ativador de protrombina Veneno de cobra Bothrops insularis Coagulation Endothelial cell Prothrombin activator Snake venom Metalloprotease |
| title_short |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| title_full |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| title_fullStr |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| title_full_unstemmed |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| title_sort |
Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains |
| author |
Modesto, Jeanne Claíne de Albuquerque |
| author_facet |
Modesto, Jeanne Claíne de Albuquerque Azevedo, Inácio Loióla Meireles Junqueira de Ferreira, Ana Gisele C. Neves Fritzen, Márcio Oliva, Maria Luíza Vilela Ho, Paulo Lee Perales, Jonas Chudzinski-Tavassi, Ana Marisa |
| author_role |
author |
| author2 |
Azevedo, Inácio Loióla Meireles Junqueira de Ferreira, Ana Gisele C. Neves Fritzen, Márcio Oliva, Maria Luíza Vilela Ho, Paulo Lee Perales, Jonas Chudzinski-Tavassi, Ana Marisa |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Modesto, Jeanne Claíne de Albuquerque Azevedo, Inácio Loióla Meireles Junqueira de Ferreira, Ana Gisele C. Neves Fritzen, Márcio Oliva, Maria Luíza Vilela Ho, Paulo Lee Perales, Jonas Chudzinski-Tavassi, Ana Marisa |
| dc.subject.other.pt_BR.fl_str_mv |
Bothrops insularis Coagulação Endothelial cell Metaloprotease Ativador de protrombina Veneno de cobra |
| topic |
Bothrops insularis Coagulação Endothelial cell Metaloprotease Ativador de protrombina Veneno de cobra Bothrops insularis Coagulation Endothelial cell Prothrombin activator Snake venom Metalloprotease |
| dc.subject.en.pt_BR.fl_str_mv |
Bothrops insularis Coagulation Endothelial cell Prothrombin activator Snake venom Metalloprotease |
| description |
Instituto Butantan. Laboratório de Bioquímica e Biofísica. São Paulo, SP, Brasil. |
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2005 |
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2005 |
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2019-12-19T12:24:49Z |
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2019-12-19T12:24:49Z |
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info:eu-repo/semantics/article |
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article |
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MODESTO, Jeanne Claine de Albuquerque et al. Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains. Biological Chemistry, v. 386, p. 589-600, June 2005. |
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https://www.arca.fiocruz.br/handle/icict/38700 |
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1431-6730 |
| dc.identifier.doi.none.fl_str_mv |
10.1515/BC.2005.069 |
| dc.identifier.eissn.none.fl_str_mv |
1437-4315 |
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MODESTO, Jeanne Claine de Albuquerque et al. Insularinase A, a prothrombin activator from Bothrops insularis venom, is a metalloprotease derived from a gene encoding protease and disintegrin domains. Biological Chemistry, v. 386, p. 589-600, June 2005. 1431-6730 10.1515/BC.2005.069 1437-4315 |
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De Gruyter |
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De Gruyter |
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