eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23309 |
Resumo: | Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
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Freire, Eden R.Vashisht, Ajay A.Malvezzi, Amaranta M.Zuberek, JoannaLangousis, GerasimosSaada, Edwin A.Nascimento, Janaína de F.Stepinski, JanuszDarzynkiewicz, EdwardHill, KentMelo Neto, Osvaldo P. deWohlschlegel, James A.Sturm, Nancy R.Campbell, David A.2017-11-22T14:14:50Z2017-11-22T14:14:50Z2014FREIRE, E. R. et al. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei. RNA (New York, N.Y.), v. 20, n. 8, p. 1272–1286, ago. 2014.1469-9001https://www.arca.fiocruz.br/handle/icict/2330910.1261/rna.045534.114engProteínas 14-3-3CinetoplastoPAC mRNAmotilidade socialFator de iniciação da tradução14-3-3 proteinkinetoplastidmRNA capsocial motilitytranslation initiation factorFator de Iniciação 4E em EucariotosmetabolismoFator de Iniciação 4G em EucariotosmetabolismoProteínas de Ligação ao Cap de RNAmetabolismoProcessamento Pós-Transcricional do RNASequência de aminoácidosCapuzes de RNAmetabolismoTécnicas de Inativação de GenesHumanosDados de Sequência MolecularLigação ProteicaRNA de ProtozoáriometabolismoeIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma bruceiinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.Members of the eIF4E mRNA cap-binding family are involved in translation and the modulation of transcript availability in other systems as part of a three-component complex including eIF4G and eIF4A. The kinetoplastids possess four described eIF4E and five eIF4G homologs. We have identified two new eIF4E family proteins in Trypanosoma brucei, and define distinct complexes associated with the fifth member, TbEIF4E5. The cytosolic TbEIF4E5 protein binds cap 0 in vitro. TbEIF4E5 was found in association with two of the five TbEIF4Gs. TbIF4EG1 bound TbEIF4E5, a 47.5-kDa protein with two RNA-binding domains, and either the regulatory protein 14-3-3 II or a 117.5-kDa protein with guanylyltransferase and methyltransferase domains in a potentially dynamic interaction. The TbEIF4G2/TbEIF4E5 complex was associated with a 17.9-kDa hypothetical protein and both 14-3-3 variants I and II. Knockdown of TbEIF4E5 resulted in the loss of productive cell movement, as evidenced by the inability of the cells to remain in suspension in liquid culture and the loss of social motility on semisolid plating medium, as well as a minor reduction of translation. Cells appeared lethargic, as opposed to compromised in flagellar function per se. The minimal use of transcriptional control in kinetoplastids requires these organisms to implement downstream mechanisms to regulate gene expression, and the TbEIF4E5/TbEIF4G1/117.5-kDa complex in particular may be a key player in that process. We suggest that a pathway involved in cell motility is affected, directly or indirectly, by one of the TbEIF4E5 complexes.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23309/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. eIF4F-like complexes - freire.pdfart. eIF4F-like complexes - freire.pdfapplication/pdf644818https://www.arca.fiocruz.br/bitstream/icict/23309/2/art.%20eIF4F-like%20complexes%20-%20freire.pdfd8f56da408fd587a3780a4c7d14b2417MD52TEXTart. eIF4F-like complexes - freire.pdf.txtart. eIF4F-like complexes - freire.pdf.txtExtracted texttext/plain85635https://www.arca.fiocruz.br/bitstream/icict/23309/3/art.%20eIF4F-like%20complexes%20-%20freire.pdf.txt5668065de2da0279cac62fd99e8e1e0cMD53icict/233092018-08-15 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dc.title.pt_BR.fl_str_mv |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
title |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
spellingShingle |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei Freire, Eden R. Proteínas 14-3-3 Cinetoplasto PAC mRNA motilidade social Fator de iniciação da tradução 14-3-3 protein kinetoplastid mRNA cap social motility translation initiation factor Fator de Iniciação 4E em Eucariotos metabolismo Fator de Iniciação 4G em Eucariotos metabolismo Proteínas de Ligação ao Cap de RNA metabolismo Processamento Pós-Transcricional do RNA Sequência de aminoácidos Capuzes de RNA metabolismo Técnicas de Inativação de Genes Humanos Dados de Sequência Molecular Ligação Proteica RNA de Protozoário metabolismo |
title_short |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
title_full |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
title_fullStr |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
title_full_unstemmed |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
title_sort |
eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei |
author |
Freire, Eden R. |
author_facet |
Freire, Eden R. Vashisht, Ajay A. Malvezzi, Amaranta M. Zuberek, Joanna Langousis, Gerasimos Saada, Edwin A. Nascimento, Janaína de F. Stepinski, Janusz Darzynkiewicz, Edward Hill, Kent Melo Neto, Osvaldo P. de Wohlschlegel, James A. Sturm, Nancy R. Campbell, David A. |
author_role |
author |
author2 |
Vashisht, Ajay A. Malvezzi, Amaranta M. Zuberek, Joanna Langousis, Gerasimos Saada, Edwin A. Nascimento, Janaína de F. Stepinski, Janusz Darzynkiewicz, Edward Hill, Kent Melo Neto, Osvaldo P. de Wohlschlegel, James A. Sturm, Nancy R. Campbell, David A. |
author2_role |
author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Freire, Eden R. Vashisht, Ajay A. Malvezzi, Amaranta M. Zuberek, Joanna Langousis, Gerasimos Saada, Edwin A. Nascimento, Janaína de F. Stepinski, Janusz Darzynkiewicz, Edward Hill, Kent Melo Neto, Osvaldo P. de Wohlschlegel, James A. Sturm, Nancy R. Campbell, David A. |
dc.subject.other.pt_BR.fl_str_mv |
Proteínas 14-3-3 Cinetoplasto PAC mRNA motilidade social Fator de iniciação da tradução |
topic |
Proteínas 14-3-3 Cinetoplasto PAC mRNA motilidade social Fator de iniciação da tradução 14-3-3 protein kinetoplastid mRNA cap social motility translation initiation factor Fator de Iniciação 4E em Eucariotos metabolismo Fator de Iniciação 4G em Eucariotos metabolismo Proteínas de Ligação ao Cap de RNA metabolismo Processamento Pós-Transcricional do RNA Sequência de aminoácidos Capuzes de RNA metabolismo Técnicas de Inativação de Genes Humanos Dados de Sequência Molecular Ligação Proteica RNA de Protozoário metabolismo |
dc.subject.en.pt_BR.fl_str_mv |
14-3-3 protein kinetoplastid mRNA cap social motility translation initiation factor |
dc.subject.decs.pt_BR.fl_str_mv |
Fator de Iniciação 4E em Eucariotos metabolismo Fator de Iniciação 4G em Eucariotos metabolismo Proteínas de Ligação ao Cap de RNA metabolismo Processamento Pós-Transcricional do RNA Sequência de aminoácidos Capuzes de RNA metabolismo Técnicas de Inativação de Genes Humanos Dados de Sequência Molecular Ligação Proteica RNA de Protozoário metabolismo |
description |
Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil. |
publishDate |
2014 |
dc.date.issued.fl_str_mv |
2014 |
dc.date.accessioned.fl_str_mv |
2017-11-22T14:14:50Z |
dc.date.available.fl_str_mv |
2017-11-22T14:14:50Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
FREIRE, E. R. et al. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei. RNA (New York, N.Y.), v. 20, n. 8, p. 1272–1286, ago. 2014. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/23309 |
dc.identifier.issn.pt_BR.fl_str_mv |
1469-9001 |
dc.identifier.doi.none.fl_str_mv |
10.1261/rna.045534.114 |
identifier_str_mv |
FREIRE, E. R. et al. eIF4F-like complexes formed by cap-binding homolog TbEIF4E5 with TbEIF4G1 or TbEIF4G2 are implicated in post-transcriptional regulation in Trypanosoma brucei. RNA (New York, N.Y.), v. 20, n. 8, p. 1272–1286, ago. 2014. 1469-9001 10.1261/rna.045534.114 |
url |
https://www.arca.fiocruz.br/handle/icict/23309 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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