Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil

Detalhes bibliográficos
Autor(a) principal: Rolando, Roberta Flávia Ribeiro
Data de Publicação: 2012
Outros Autores: Silva, Sidnei da, Peralta, Regina Helena Saramago, Silva, Alexandre Januário da, Cunha, Flavia de Souza, Bello, Alexandre Ribeiro, Peralta, José Mauro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36618
Resumo: Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
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spelling Rolando, Roberta Flávia RibeiroSilva, Sidnei daPeralta, Regina Helena SaramagoSilva, Alexandre Januário daCunha, Flavia de SouzaBello, Alexandre RibeiroPeralta, José Mauro2019-10-22T12:07:41Z2019-10-22T12:07:41Z2012ROLANDO, Roberta Flávia Ribeiro et al. Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 476-479, June 2012.0074-0276https://www.arca.fiocruz.br/handle/icict/3661810.1590/S0074-027620120004000061678-8060engInstituto Oswaldo Cruz, Ministério da SaúdeDetection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Centers for Disease Control and Prevention. Center for Global Health. Division of Parasitic Diseases and Malaria. Atlanta, GA, USAUniversidade Federal Fluminense. Faculdade de Medicina. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.This study reports the first genetic characterisation of Cryptosporidium isolates in Brazil using real-time polymerase chain reaction (RT-PCR). A total of 1,197 faecal specimens from children and 10 specimens from human immunodeficiency virus-infected patients were collected between 1999-2010 and screened using microscopy. Forty-eight Cryptosporidium oocyst-positive isolates were identified and analysed using a generic TaqMan assay targeting the 18S rRNA to detect Cryptosporidium species and two other TaqMan assays to identify Cryptosporidium hominis and Cryptosporidium parvum. The 18S rRNA assay detected Cryptosporidium species in all 48 of the stool specimens. The C. parvum TaqMan assay correctly identified five/48 stool samples, while 37/48 stool specimens were correctly amplified in the C. hominis TaqMan assay. The results obtained in this study support previous findings showing that C. hominis infections are more prevalent than C. parvum infections in Brazil and they demonstrate that the TaqMan RT-PCR procedure is a simple, fast and valuable tool for the detection and differentiation of Cryptosporidium species.CryptosporidiumRT-PCRMolecular epidemiologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36618/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Rolando_Roberta_etal_INI_2012.pdfve_Rolando_Roberta_etal_INI_2012.pdfapplication/pdf292362https://www.arca.fiocruz.br/bitstream/icict/36618/2/ve_Rolando_Roberta_etal_INI_2012.pdf11ca1f9fc99721796dc5e48896e2b81dMD52TEXTve_Rolando_Roberta_etal_INI_2012.pdf.txtve_Rolando_Roberta_etal_INI_2012.pdf.txtExtracted texttext/plain22743https://www.arca.fiocruz.br/bitstream/icict/36618/3/ve_Rolando_Roberta_etal_INI_2012.pdf.txtd619105534a473959bf84eb1ce55e15bMD53icict/366182019-10-23 02:02:33.896oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-10-23T05:02:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
title Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
spellingShingle Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
Rolando, Roberta Flávia Ribeiro
Cryptosporidium
RT-PCR
Molecular epidemiology
title_short Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
title_full Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
title_fullStr Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
title_full_unstemmed Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
title_sort Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil
author Rolando, Roberta Flávia Ribeiro
author_facet Rolando, Roberta Flávia Ribeiro
Silva, Sidnei da
Peralta, Regina Helena Saramago
Silva, Alexandre Januário da
Cunha, Flavia de Souza
Bello, Alexandre Ribeiro
Peralta, José Mauro
author_role author
author2 Silva, Sidnei da
Peralta, Regina Helena Saramago
Silva, Alexandre Januário da
Cunha, Flavia de Souza
Bello, Alexandre Ribeiro
Peralta, José Mauro
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rolando, Roberta Flávia Ribeiro
Silva, Sidnei da
Peralta, Regina Helena Saramago
Silva, Alexandre Januário da
Cunha, Flavia de Souza
Bello, Alexandre Ribeiro
Peralta, José Mauro
dc.subject.en.pt_BR.fl_str_mv Cryptosporidium
RT-PCR
Molecular epidemiology
topic Cryptosporidium
RT-PCR
Molecular epidemiology
description Universidade do Estado do Rio de Janeiro. Departamento de Microbiologia, Imunologia e Parasitologia. Rio de Janeiro, RJ, Brasil.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-10-22T12:07:41Z
dc.date.available.fl_str_mv 2019-10-22T12:07:41Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv ROLANDO, Roberta Flávia Ribeiro et al. Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 476-479, June 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36618
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762012000400006
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv ROLANDO, Roberta Flávia Ribeiro et al. Detection and differentiation of Cryptosporidium by real-time polymerase chain reaction in stool samples from patients in Rio de Janeiro, Brazil. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 107, n. 4, p. 476-479, June 2012.
0074-0276
10.1590/S0074-02762012000400006
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36618
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
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