Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP

Detalhes bibliográficos
Autor(a) principal: Carvalho, Rodrigo
Data de Publicação: 2018
Outros Autores: Vaz, Aline, Pereira, Felipe Luiz, Dorella, Fernanda, Aguiar, Eric, Chatel, Jean-Marc, Bermudez, Luis, Langella, Philippe, Fernandes, Gabriel da Rocha, Figueiredo, Henrique, Goes Neto, Aristóteles, Azevedo, Vasco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33772
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
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spelling Carvalho, RodrigoVaz, AlinePereira, Felipe LuizDorella, FernandaAguiar, EricChatel, Jean-MarcBermudez, LuisLangella, PhilippeFernandes, Gabriel da RochaFigueiredo, HenriqueGoes Neto, AristótelesAzevedo, Vasco2019-07-02T13:57:07Z2019-07-02T13:57:07Z2018CARVALHO, Rodrigo et al. Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP. Scientific Reports, v. 8, n. 1, p. 1-10, 2018.2045-2322https://www.arca.fiocruz.br/handle/icict/3377210.1038/s41598-018-33469-wengNature Publishing GroupGut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAPinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal da Bahia. Salvador, BA, Brasil.Micalis Institute. INRA, AgroParisTech. Université Paris-Saclay. Jouy-en-Josas, France.Micalis Institute. INRA, AgroParisTech. Université Paris-Saclay. Jouy-en-Josas, France.Micalis Institute. INRA, AgroParisTech. Université Paris-Saclay. Jouy-en-Josas, France.Fundação Oswaldo Cruz. Instituto Renê Rachou. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.Mucositis is an inflammatory condition of the gut, caused by an adverse effect of chemotherapy drugs, such as 5-fluorouracil (5-FU). In an attempt to develop alternative treatments for the disease, several research groups have proposed the use of probiotics, in particular, Lactic Acid Bacteria (LAB). In this context, the use of recombinant LAB, for delivering anti-inflammatory compounds has also been explored. In previous work, we demonstrated that either Lactococcus lactis NZ9000 or a recombinant strain expressing an antimicrobial peptide involved in human gut homeostasis, the Pancreatitis-associated Protein (PAP), could ameliorate 5-FU-induced mucositis in mice. However, the impact of these strains on the gut microbiota still needs to be elucidated. Therefore, in the present study, we aimed to characterize the effects of both Lactococci strains in the gut microbiome of mice through a 16 S rRNA gene sequencing metagenomic approach. Our data show 5-FU caused a significant decrease in protective bacteria and increase of several bacteria associated with pro-inflammatory traits. The Lactococci strains were shown to reduce several potential opportunistic microbes, while PAP delivery was able to suppress the growth of Enterobacteriaceae during inflammation. We conclude the strain secreting antimicrobial PAP was more effective in the control of 5-FU-dysbiosis.Mucositisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/33772/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALGut microbiome modulation during treatment.pdfGut microbiome modulation during treatment.pdfapplication/pdf6433310https://www.arca.fiocruz.br/bitstream/icict/33772/2/Gut%20microbiome%20modulation%20during%20treatment.pdff3546f8cdfbb7cdbd857e994a3c06f6cMD52TEXTGut microbiome modulation during treatment.pdf.txtGut microbiome modulation during treatment.pdf.txtExtracted texttext/plain2300https://www.arca.fiocruz.br/bitstream/icict/33772/3/Gut%20microbiome%20modulation%20during%20treatment.pdf.txt9d65657f7aaf38ffd94080e8456b11beMD53icict/337722019-07-03 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dc.title.pt_BR.fl_str_mv Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
title Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
spellingShingle Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
Carvalho, Rodrigo
Mucositis
title_short Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
title_full Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
title_fullStr Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
title_full_unstemmed Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
title_sort Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP
author Carvalho, Rodrigo
author_facet Carvalho, Rodrigo
Vaz, Aline
Pereira, Felipe Luiz
Dorella, Fernanda
Aguiar, Eric
Chatel, Jean-Marc
Bermudez, Luis
Langella, Philippe
Fernandes, Gabriel da Rocha
Figueiredo, Henrique
Goes Neto, Aristóteles
Azevedo, Vasco
author_role author
author2 Vaz, Aline
Pereira, Felipe Luiz
Dorella, Fernanda
Aguiar, Eric
Chatel, Jean-Marc
Bermudez, Luis
Langella, Philippe
Fernandes, Gabriel da Rocha
Figueiredo, Henrique
Goes Neto, Aristóteles
Azevedo, Vasco
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Carvalho, Rodrigo
Vaz, Aline
Pereira, Felipe Luiz
Dorella, Fernanda
Aguiar, Eric
Chatel, Jean-Marc
Bermudez, Luis
Langella, Philippe
Fernandes, Gabriel da Rocha
Figueiredo, Henrique
Goes Neto, Aristóteles
Azevedo, Vasco
dc.subject.en.pt_BR.fl_str_mv Mucositis
topic Mucositis
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-07-02T13:57:07Z
dc.date.available.fl_str_mv 2019-07-02T13:57:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Rodrigo et al. Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP. Scientific Reports, v. 8, n. 1, p. 1-10, 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33772
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-018-33469-w
identifier_str_mv CARVALHO, Rodrigo et al. Gut microbiome modulation during treatment of mucositis with the dairy bacterium Lactococcus lactis and recombinant strain secreting human antimicrobial PAP. Scientific Reports, v. 8, n. 1, p. 1-10, 2018.
2045-2322
10.1038/s41598-018-33469-w
url https://www.arca.fiocruz.br/handle/icict/33772
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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