Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway

Detalhes bibliográficos
Autor(a) principal: Andrade, Luiza Freire de
Data de Publicação: 2014
Outros Autores: Mourão, Marina de Moraes, Geraldo, Juliana Assis, Coelho, Fernanda Sales, Silva, Larissa Lopes, Neves, Renata Heisler, Volpini, Angela Cristina, Silva, José Roberto Machado, Araujo, Neusa, Pimenta, Rafael Nacif, Caffrey, Conor R., Oliveira, Guilherme Corrêa de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
DOI: 10.1371/journal.pntd.0002949
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9497
Resumo: Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.
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spelling Andrade, Luiza Freire deMourão, Marina de MoraesGeraldo, Juliana AssisCoelho, Fernanda SalesSilva, Larissa LopesNeves, Renata HeislerVolpini, Angela CristinaSilva, José Roberto MachadoAraujo, NeusaPimenta, Rafael NacifCaffrey, Conor R.Oliveira, Guilherme Corrêa de2015-02-13T14:55:55Z2015-02-13T14:55:55Z2014ANDRADE, Luiza Freire de et al. Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway. PLoS Neglected Tropical Diseases, v. 8, n. 6, p. 1-12, 2014.1553-7366https://www.arca.fiocruz.br/handle/icict/949710.1371/journal.pntd.0002949engPublic Library of ScienceRegulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathwayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Ensino. Biologia Parasitaria. Rio de Janeiro, RJ, Brasil / Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Medicas. Departamento de Patologia e Laboratórios. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Medicas. Departamento de Patologia e Laboratórios. Laboratório de Helmintologia Romero Lascasas Porto. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Entomologia Medica. Belo Horizonte, MG, Brasil.University of California. California Institute for Quantitative Biosciences and the Department of Pathology. San Francisco, CA, USA.Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.BACKGROUND: Protein kinases are proven targets for drug development with an increasing number of eukaryotic Protein Kinase (ePK) inhibitors now approved as drugs. Mitogen-activated protein kinase (MAPK) family members connect cell-surface receptors to regulatory targets within cells and influence a number of tissue-specific biological activities such as cell proliferation, differentiation and survival. However, the contributions of members of the MAPK pathway to schistosome development and survival are unclear. METHODOLOGY/PRINCIPAL FINDINGS: We employed RNA interference (RNAi) to elucidate the functional roles of five S. mansoni genes (SmCaMK2, SmJNK, SmERK1, SmERK2 and SmRas) involved in MAPK signaling pathway. Mice were injected with post-infective larvae (schistosomula) subsequent to RNAi and the development of adult worms observed. The data demonstrate that SmJNK participates in parasite maturation and survival of the parasites, whereas SmERK are involved in egg production as infected mice had significantly lower egg burdens with female worms presenting underdeveloped ovaries. Furthermore, it was shown that the c-fos transcription factor was overexpressed in parasites submitted to RNAi of SmERK1, SmJNK and SmCaMK2 indicating its putative involvement in gene regulation in this parasite's MAPK signaling cascade. CONCLUSIONS: We conclude that MAPKs proteins play important roles in the parasite in vivo survival, being essential for normal development and successful survival and reproduction of the schistosome parasite. Moreover SmERK and SmJNK are potential targets for drug development.Schistosoma mansoniSchistosomiasis controlMAP kinasesMAPKsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9497/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_078.pdf2014_078.pdfapplication/pdf2052169https://www.arca.fiocruz.br/bitstream/icict/9497/2/2014_078.pdfa893a56a23ecf0276c3dd34e4863af08MD52TEXT2014_078.pdf.txt2014_078.pdf.txtExtracted texttext/plain56082https://www.arca.fiocruz.br/bitstream/icict/9497/3/2014_078.pdf.txt5aa1291d87142781c88ad950deae7a72MD53icict/94972023-09-14 16:02:03.251oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T19:02:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
title Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
spellingShingle Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
Andrade, Luiza Freire de
Schistosoma mansoni
Schistosomiasis control
MAP kinases
MAPKs
title_short Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
title_full Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
title_fullStr Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
title_full_unstemmed Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
title_sort Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway
author Andrade, Luiza Freire de
author_facet Andrade, Luiza Freire de
Mourão, Marina de Moraes
Geraldo, Juliana Assis
Coelho, Fernanda Sales
Silva, Larissa Lopes
Neves, Renata Heisler
Volpini, Angela Cristina
Silva, José Roberto Machado
Araujo, Neusa
Pimenta, Rafael Nacif
Caffrey, Conor R.
Oliveira, Guilherme Corrêa de
author_role author
author2 Mourão, Marina de Moraes
Geraldo, Juliana Assis
Coelho, Fernanda Sales
Silva, Larissa Lopes
Neves, Renata Heisler
Volpini, Angela Cristina
Silva, José Roberto Machado
Araujo, Neusa
Pimenta, Rafael Nacif
Caffrey, Conor R.
Oliveira, Guilherme Corrêa de
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Luiza Freire de
Mourão, Marina de Moraes
Geraldo, Juliana Assis
Coelho, Fernanda Sales
Silva, Larissa Lopes
Neves, Renata Heisler
Volpini, Angela Cristina
Silva, José Roberto Machado
Araujo, Neusa
Pimenta, Rafael Nacif
Caffrey, Conor R.
Oliveira, Guilherme Corrêa de
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Schistosomiasis control
MAP kinases
MAPKs
topic Schistosoma mansoni
Schistosomiasis control
MAP kinases
MAPKs
description Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Rene Rachou. Centro de Excelência em Bioinformática. Belo Horizonte, MG, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-02-13T14:55:55Z
dc.date.available.fl_str_mv 2015-02-13T14:55:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANDRADE, Luiza Freire de et al. Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway. PLoS Neglected Tropical Diseases, v. 8, n. 6, p. 1-12, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9497
dc.identifier.issn.none.fl_str_mv 1553-7366
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0002949
identifier_str_mv ANDRADE, Luiza Freire de et al. Regulation of Schistosoma mansoni Development and Reproduction by the Mitogen-Activated Protein Kinase Signaling Pathway. PLoS Neglected Tropical Diseases, v. 8, n. 6, p. 1-12, 2014.
1553-7366
10.1371/journal.pntd.0002949
url https://www.arca.fiocruz.br/handle/icict/9497
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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