Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.

Santos, Fred Luciano Neves; Gonçalves, Marilda de Souza; Soares, Neci Matos

Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.

Detalhes bibliográficos
Autor(a) principal:	Santos, Fred Luciano Neves
Data de Publicação:	2011
Outros Autores:	Gonçalves, Marilda de Souza, Soares, Neci Matos
Tipo de documento:	Artigo
Idioma:	eng
Título da fonte:	Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo:	https://www.arca.fiocruz.br/handle/icict/7947
Resumo:	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Health and Investigative Medicine Biotechnology . Salvador, BA, Brasil. Salvador, BA, Brasil

Metadados do item

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spelling	Santos, Fred Luciano NevesGonçalves, Marilda de SouzaSoares, Neci Matos2014-07-14T14:41:48Z2014-07-14T14:41:48Z2011SANTOS, F. L. N.; GONÇALVES, M. S.; SOARES, N. M. Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Brazilian Journal of Infectious Disease, v. 15, n. 2, p. 119-125, 2011 .1678-4391https://www.arca.fiocruz.br/handle/icict/7947engElsevierValidation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Health and Investigative Medicine Biotechnology . Salvador, BA, Brasil. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratory of Pathology and Molecular Biology. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilAmoebiasis is an infection caused by Entamoeba histolytica and is a potential health risk in countries in which health barriers are inappropriate. Since the discovery of Entamoeba dispar, the prevalence of amoebiasis has been modified. OBJECTIVE: This study has standardized the PCR technique applied for the diagnosis of different species of the E. histolytica/E. dispar complex and has evaluated the prevalence of infection among patients attending private and public clinical laboratories in Salvador City, Bahia State, Brazil. RESULTS: Analysis of 52,704 stool samples by microscopic examination demonstrated that 1,788 (3.4%) were positive for the E. histolytica/E. dispar complex and infection occurred more often in samples originated from public clinical laboratories (5.0%) than those that came from private laboratories (3.2%). PCR performed in approximately 15% (262) E. histolytica/E. dispar complex positive samples, randomly chosen, amplified 227 samples (86.6%), all of them positive for E. dispar. The non-amplified 35 samples (13.4%) were also negative for E. histolytica-specific galactose adhesin. Moreover, to exclude a probable infection caused by E. hartmanni, morphometric analysis demonstrated that non-amplified samples had cyst sizes comparable to E. histolytica/E. dispar (>10 µm). CONCLUSION: The absence of amplification of these samples indicates the presence of PCR inhibitors in the stool samples or the presence of DNA from Entamoeba species other than E. dispar, E. histolytica or E. hartmanni.AmebiasisEntamoeba histolyticaDiagnosisPrevalenceEntamoeba/genéticaEntamebíase/diagnósticoEntamebíase/parasitologiaReação em Cadeia da Polimerase/métodosBrasil/epidemiologiaDNA de Protozoário/análiseDiagnóstico DiferencialEntamoeba/classificaçãoEntamoeba/isolamento & purificaçãoEntamoeba histolytica/genéticaEntamoeba histolytica/isolamento & purificaçãoEntamebíase/epidemiologiaFezes/parasitologiaHumanosPrevalênciaSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSantos, FL. Validation and utilization....pdfSantos, FL. 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dc.title.pt_BR.fl_str_mv	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
title	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
spellingShingle	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Santos, Fred Luciano Neves Amebiasis Entamoeba histolytica Diagnosis Prevalence Entamoeba/genética Entamebíase/diagnóstico Entamebíase/parasitologia Reação em Cadeia da Polimerase/métodos Brasil/epidemiologia DNA de Protozoário/análise Diagnóstico Diferencial Entamoeba/classificação Entamoeba/isolamento & purificação Entamoeba histolytica/genética Entamoeba histolytica/isolamento & purificação Entamebíase/epidemiologia Fezes/parasitologia Humanos Prevalência Sensibilidade e Especificidade
title_short	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
title_full	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
title_fullStr	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
title_full_unstemmed	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
title_sort	Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.
author	Santos, Fred Luciano Neves
author_facet	Santos, Fred Luciano Neves Gonçalves, Marilda de Souza Soares, Neci Matos
author_role	author
author2	Gonçalves, Marilda de Souza Soares, Neci Matos
author2_role	author author
dc.contributor.author.fl_str_mv	Santos, Fred Luciano Neves Gonçalves, Marilda de Souza Soares, Neci Matos
dc.subject.en.pt_BR.fl_str_mv	Amebiasis Entamoeba histolytica Diagnosis Prevalence
topic	Amebiasis Entamoeba histolytica Diagnosis Prevalence Entamoeba/genética Entamebíase/diagnóstico Entamebíase/parasitologia Reação em Cadeia da Polimerase/métodos Brasil/epidemiologia DNA de Protozoário/análise Diagnóstico Diferencial Entamoeba/classificação Entamoeba/isolamento & purificação Entamoeba histolytica/genética Entamoeba histolytica/isolamento & purificação Entamebíase/epidemiologia Fezes/parasitologia Humanos Prevalência Sensibilidade e Especificidade
dc.subject.decs.pt_BR.fl_str_mv	Entamoeba/genética Entamebíase/diagnóstico Entamebíase/parasitologia Reação em Cadeia da Polimerase/métodos Brasil/epidemiologia DNA de Protozoário/análise Diagnóstico Diferencial Entamoeba/classificação Entamoeba/isolamento & purificação Entamoeba histolytica/genética Entamoeba histolytica/isolamento & purificação Entamebíase/epidemiologia Fezes/parasitologia Humanos Prevalência Sensibilidade e Especificidade
description	Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Health and Investigative Medicine Biotechnology . Salvador, BA, Brasil. Salvador, BA, Brasil
publishDate	2011
dc.date.issued.fl_str_mv	2011
dc.date.accessioned.fl_str_mv	2014-07-14T14:41:48Z
dc.date.available.fl_str_mv	2014-07-14T14:41:48Z
dc.type.status.fl_str_mv	info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv	info:eu-repo/semantics/article
format	article
status_str	publishedVersion
dc.identifier.citation.fl_str_mv	SANTOS, F. L. N.; GONÇALVES, M. S.; SOARES, N. M. Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Brazilian Journal of Infectious Disease, v. 15, n. 2, p. 119-125, 2011 .
dc.identifier.uri.fl_str_mv	https://www.arca.fiocruz.br/handle/icict/7947
dc.identifier.issn.none.fl_str_mv	1678-4391
identifier_str_mv	SANTOS, F. L. N.; GONÇALVES, M. S.; SOARES, N. M. Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil. Brazilian Journal of Infectious Disease, v. 15, n. 2, p. 119-125, 2011 . 1678-4391
url	https://www.arca.fiocruz.br/handle/icict/7947
dc.language.iso.fl_str_mv	eng
language	eng
dc.rights.driver.fl_str_mv	info:eu-repo/semantics/openAccess
eu_rights_str_mv	openAccess
dc.publisher.none.fl_str_mv	Elsevier
publisher.none.fl_str_mv	Elsevier
dc.source.none.fl_str_mv	reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ
instname_str	Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str	FIOCRUZ
institution	FIOCRUZ
reponame_str	Repositório Institucional da FIOCRUZ (ARCA)
collection	Repositório Institucional da FIOCRUZ (ARCA)
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Validation and utilization of PCR for differential diagnosis and prevalence determination of Entamoeba histolytica/Entamoeba dispar in Salvador City, Brazil.

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