Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis

Detalhes bibliográficos
Autor(a) principal: Almeida, Isabela Neves de
Data de Publicação: 2019
Outros Autores: Vasconcellos, Sidra Ezidio Gonçalves, Figueredo, Lida Jouca de Assis, Dantas, Nayanne Gama Teixeira, Augusto, Cláudio José, Hadaad, João Paulo Amaral, Suffys, Philip Noel, Carvalho, Wânia da Silva, Miranda, Silvana Spíndola de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40548
Resumo: Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.
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spelling Almeida, Isabela Neves deVasconcellos, Sidra Ezidio GonçalvesFigueredo, Lida Jouca de AssisDantas, Nayanne Gama TeixeiraAugusto, Cláudio JoséHadaad, João Paulo AmaralSuffys, Philip NoelCarvalho, Wânia da SilvaMiranda, Silvana Spíndola de2020-03-28T17:10:51Z2020-03-28T17:10:51Z2019ALMEIDA, Isabela Neves et al. Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis. BMC Infectious Diseases, v. 18, n. 556, p. 1-9, 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/4054810.1186/s12879-019-4152-7engBMCTuberculoseMycobacterium tuberculosisGenótipoTuberculose Resistente a Múltiplos MedicamentosNucleotídeo únicoTuberculosisMycobacterium tuberculosisMultidrug-resistant tuberculosisGenotypeSingle nucleotideFrequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Escola de Veterinária. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.Background: In recent decades, Mycobacterium tuberculosis with the RDRio genotype, frequently isolated from tuberculosis patients in Rio de Janeiro, has become part of the Latin American – Mediterranean (LAM) family and has been associated with multidrug-resistant tuberculosis (MDR-TB). The aim of this study was to investigate the frequency of M. tuberculosis RDRio in the state of Minas Gerais, Brazil, and its relationship with MDR-TB. Methods: For convenience, 172 susceptible and 63 MDR M. tuberculosis isolates were taken from pulmonary samples from patients diagnosed between January 2007 and December 2011. The DNA extracted from these isolates was analyzed by spoligotyping, PCR-RFLP to characterize fbpC103/Ag85C103, multiplex PCR to detect RDRio and RD174, and MIRU-VNTR 24 loci. Results: Among the 235 isolates, the RDRio pattern was identified in 122 (51.9%) isolates (IC 0.45–0.58), with 100 (42.5%) wild-type and 13 (5.5%) mixed pattern isolates, whereas RD174 was identified in 93 of the 122 RDRio positive samples (76.3%). The LAM family and the LAM9 lineage were the most frequently identified among the isolates in this study. Among the 63 MDR isolates, 41 (65.1%) were RDRio and 28 (44.4%) RD174. Conclusion: The association of both deletions with MDR proved to be statistically significant, corroborating the few reports that have associated RDRio with MDR.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40548/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPhilipNSuffys_SEGVasconcelos_etal_IOC_2019.pdfPhilipNSuffys_SEGVasconcelos_etal_IOC_2019.pdfapplication/pdf2891235https://www.arca.fiocruz.br/bitstream/icict/40548/2/PhilipNSuffys_SEGVasconcelos_etal_IOC_2019.pdf2bb6f334e755ecd55c7914668a54beb7MD52TEXTPhilipNSuffys_SEGVasconcelos_etal_IOC_2019.pdf.txtPhilipNSuffys_SEGVasconcelos_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
title Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
spellingShingle Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
Almeida, Isabela Neves de
Tuberculose
Mycobacterium tuberculosis
Genótipo
Tuberculose Resistente a Múltiplos Medicamentos
Nucleotídeo único
Tuberculosis
Mycobacterium tuberculosis
Multidrug-resistant tuberculosis
Genotype
Single nucleotide
title_short Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
title_full Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
title_fullStr Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
title_full_unstemmed Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
title_sort Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis
author Almeida, Isabela Neves de
author_facet Almeida, Isabela Neves de
Vasconcellos, Sidra Ezidio Gonçalves
Figueredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Augusto, Cláudio José
Hadaad, João Paulo Amaral
Suffys, Philip Noel
Carvalho, Wânia da Silva
Miranda, Silvana Spíndola de
author_role author
author2 Vasconcellos, Sidra Ezidio Gonçalves
Figueredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Augusto, Cláudio José
Hadaad, João Paulo Amaral
Suffys, Philip Noel
Carvalho, Wânia da Silva
Miranda, Silvana Spíndola de
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Isabela Neves de
Vasconcellos, Sidra Ezidio Gonçalves
Figueredo, Lida Jouca de Assis
Dantas, Nayanne Gama Teixeira
Augusto, Cláudio José
Hadaad, João Paulo Amaral
Suffys, Philip Noel
Carvalho, Wânia da Silva
Miranda, Silvana Spíndola de
dc.subject.other.pt_BR.fl_str_mv Tuberculose
Mycobacterium tuberculosis
Genótipo
Tuberculose Resistente a Múltiplos Medicamentos
Nucleotídeo único
topic Tuberculose
Mycobacterium tuberculosis
Genótipo
Tuberculose Resistente a Múltiplos Medicamentos
Nucleotídeo único
Tuberculosis
Mycobacterium tuberculosis
Multidrug-resistant tuberculosis
Genotype
Single nucleotide
dc.subject.en.pt_BR.fl_str_mv Tuberculosis
Mycobacterium tuberculosis
Multidrug-resistant tuberculosis
Genotype
Single nucleotide
description Universidade Federal de Minas Gerais. Faculdade de Medicina. Laboratório de Pesquisa em Micobactérias. Belo Horizonte, MG, Brasil.
publishDate 2019
dc.date.issued.fl_str_mv 2019
dc.date.accessioned.fl_str_mv 2020-03-28T17:10:51Z
dc.date.available.fl_str_mv 2020-03-28T17:10:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA, Isabela Neves et al. Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis. BMC Infectious Diseases, v. 18, n. 556, p. 1-9, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40548
dc.identifier.issn.pt_BR.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-019-4152-7
identifier_str_mv ALMEIDA, Isabela Neves et al. Frequency of the Mycobacterium tuberculosis RDRio genotype and its association with multidrug-resistant tuberculosis. BMC Infectious Diseases, v. 18, n. 556, p. 1-9, 2019.
1471-2334
10.1186/s12879-019-4152-7
url https://www.arca.fiocruz.br/handle/icict/40548
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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