Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/10872 |
Resumo: | Universidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil. |
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Menezes, Francisca Gleire Rodrigues deNeves, Soraya da SilvaSousa, Oscarina Viana deVila-Nova, Candida Machado Veira MaiaMaggioni, RodrigoTheophilo, Grace Nazareth DiogoHofer, ErnestoVieira, Regine Helena Silva dos Fernandes2015-06-17T12:05:22Z2015-06-17T12:05:22Z2014MENEZES, Francisca Gleire Rodrigues de; et al. Detection of virulence genes in environmental strains of Vibrio cholerae from Estuaries in Northeastern Brasil.Rev Inst Med Trop Sao Paulo. v.56, n.5, p.427-32, Sept. Oct. 2014.0036-4665https://www.arca.fiocruz.br/handle/icict/1087210.1590/S0036-46652014000500010engInstituto de Medicina Tropical de São PauloDetection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Ceará. Instituto de Ciências do Mar (LABOMAR). Fortaleza, CE, Brasil.The objectives of this study were to detect the presence of Vibrio cholerae in tropical estuaries (Northeastern Brazil) and to search for virulence factors in the environmental isolates. Water and sediment samples were inoculated onto a vibrio-selective medium (TCBS), and colonies with morphological resemblance to V. cholerae were isolated. The cultures were identified phenotypically using a dichotomous key based on biochemical characteristics. The total DNA extracted was amplified by PCR to detect ompW and by multiplex PCR to detect the virulence genes ctx, tcp, zot and rfbO1. The results of the phenotypic and genotypic identification were compared. Nine strains of V. cholerae were identified phenotypically, five of which were confirmed by detection of the species-specific gene ompW. The dichotomous key was efficient at differentiating environmental strains of V. cholerae. Strains of V. cholerae were found in all four estuaries, but none possessed virulence genes.CholeraEstuariesPathogenicityGenesCóleraVirulênciaGenesEstuáriosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10872/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALgrace_theophiloetal_IOC_2014.pdfapplication/pdf336297https://www.arca.fiocruz.br/bitstream/icict/10872/2/grace_theophiloetal_IOC_2014.pdfa7ae7f009b170bc01eb03d5f4584f59dMD52TEXTgrace_theophiloetal_IOC_2014.pdf.txtgrace_theophiloetal_IOC_2014.pdf.txtExtracted texttext/plain27729https://www.arca.fiocruz.br/bitstream/icict/10872/3/grace_theophiloetal_IOC_2014.pdf.txt5b142c82f23ccb39e6a3268333849affMD53icict/108722022-06-24 13:08:45.237oai:www.arca.fiocruz.br:icict/10872TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:08:45Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| title |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| spellingShingle |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil Menezes, Francisca Gleire Rodrigues de Cholera Estuaries Pathogenicity Genes Cólera Virulência Genes Estuários |
| title_short |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| title_full |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| title_fullStr |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| title_full_unstemmed |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| title_sort |
Detection of virulence genes in environmental strains of vibrio cholerae from estuaries in Northeastern Brazil |
| author |
Menezes, Francisca Gleire Rodrigues de |
| author_facet |
Menezes, Francisca Gleire Rodrigues de Neves, Soraya da Silva Sousa, Oscarina Viana de Vila-Nova, Candida Machado Veira Maia Maggioni, Rodrigo Theophilo, Grace Nazareth Diogo Hofer, Ernesto Vieira, Regine Helena Silva dos Fernandes |
| author_role |
author |
| author2 |
Neves, Soraya da Silva Sousa, Oscarina Viana de Vila-Nova, Candida Machado Veira Maia Maggioni, Rodrigo Theophilo, Grace Nazareth Diogo Hofer, Ernesto Vieira, Regine Helena Silva dos Fernandes |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Menezes, Francisca Gleire Rodrigues de Neves, Soraya da Silva Sousa, Oscarina Viana de Vila-Nova, Candida Machado Veira Maia Maggioni, Rodrigo Theophilo, Grace Nazareth Diogo Hofer, Ernesto Vieira, Regine Helena Silva dos Fernandes |
| dc.subject.en.pt_BR.fl_str_mv |
Cholera Estuaries Pathogenicity Genes |
| topic |
Cholera Estuaries Pathogenicity Genes Cólera Virulência Genes Estuários |
| dc.subject.decs.pt_BR.fl_str_mv |
Cólera Virulência Genes Estuários |
| description |
Universidade Federal do Ceará. Departamento de Engenharia da Pesca. Fortaleza, CE, Brasil. |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014 |
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2015-06-17T12:05:22Z |
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2015-06-17T12:05:22Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MENEZES, Francisca Gleire Rodrigues de; et al. Detection of virulence genes in environmental strains of Vibrio cholerae from Estuaries in Northeastern Brasil.Rev Inst Med Trop Sao Paulo. v.56, n.5, p.427-32, Sept. Oct. 2014. |
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https://www.arca.fiocruz.br/handle/icict/10872 |
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0036-4665 |
| dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/S0036-46652014000500010 |
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MENEZES, Francisca Gleire Rodrigues de; et al. Detection of virulence genes in environmental strains of Vibrio cholerae from Estuaries in Northeastern Brasil.Rev Inst Med Trop Sao Paulo. v.56, n.5, p.427-32, Sept. Oct. 2014. 0036-4665 10.1590/S0036-46652014000500010 |
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