Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results

Detalhes bibliográficos
Autor(a) principal: Freixo, Maria Inês Moura
Data de Publicação: 2004
Outros Autores: Caldas, Paulo C. S., Said, Abbadi, Martins, Fátima, Brito, Rossana Coimbra, Fonsec, Leila de Souza, Saad, Maria Helena Féres
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44524
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
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spelling Freixo, Maria Inês MouraCaldas, Paulo C. S.Said, AbbadiMartins, FátimaBrito, Rossana CoimbraFonsec, Leila de SouzaSaad, Maria Helena Féres2020-11-18T20:18:28Z2020-11-18T20:18:28Z2004FREIXO, Maria Inês Moura et al. Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 107-110, Feb. 2004.0074-0276https://www.arca.fiocruz.br/handle/icict/4452410.1590/S0074-027620040001000191678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Mycobacterium tuberculosisE testTestes de Sensibilidade MicrobianaAnálise de Sequência de DNAMycobacterium tuberculosisSusceptibility testDNA sequencingE testAnálise de Sequência de DNATestes de Sensibilidade MicrobianaAntimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary resultsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Centro de Referência Prof. Hélio Fraga. Rio de Janeiro, RJ, Brasil.Suez Canal University.School of Medicine. Ismailia, Egypt.Ministério da Saúde. Centro de Referência Prof. Hélio Fraga. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Hospital Pedro Ernesto. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Medicina Tropical. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil.Mycobacterium tuberculosis strains resistant to streptomycin (SM), isoniazid (INH), and/or rifampin (RIF) as determined by the conventional Löwenstein-Jensen proportion method (LJPM) were compared with the E test, a minimum inhibitory concentration susceptibility method. Discrepant isolates were further evaluated by BACTEC and by DNA sequence analyses for mutations in genes most often associated with resistance to these drugs (rpsL, katG, inhA, and rpoB). Preliminary discordant E test results were seen in 75% of isolates resistant to SM and in 11% to INH. Discordance improved for these two drugs (63%) for SM and none for INH when isolates were re-tested but worsened for RIF (30%). Despite good agreement between phenotypic results and sequencing analyses, wild type profiles were detected on resistant strains mainly for SM and INH. It should be aware that susceptible isolates according to molecular methods might contain other mechanisms of resistance. Although reproducibility of the LJPM susceptibility method has been established, variable E test results for some M. tuberculosis isolates poses questions regarding its reproducibility particularly the impact of E test performance which may vary among laboratories despite adherence to recommended protocols. Further studies must be done to enlarge the evaluated samples and looked possible mutations outside of the hot spot sequenced gene among discrepant strains.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44524/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMariaInes_Freixo_etal_IOC_2004.pdfMariaInes_Freixo_etal_IOC_2004.pdfapplication/pdf62193https://www.arca.fiocruz.br/bitstream/icict/44524/2/MariaInes_Freixo_etal_IOC_2004.pdfd15d5e0eff3cfdf0a02b40909e72e61cMD52TEXTMariaInes_Freixo_etal_IOC_2004.pdf.txtMariaInes_Freixo_etal_IOC_2004.pdf.txtExtracted texttext/plain20422https://www.arca.fiocruz.br/bitstream/icict/44524/3/MariaInes_Freixo_etal_IOC_2004.pdf.txtc42003d1fc9ecffc92f2e3cb94a17f27MD53icict/445242020-11-19 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dc.title.pt_BR.fl_str_mv Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
title Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
spellingShingle Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
Freixo, Maria Inês Moura
Mycobacterium tuberculosis
E test
Testes de Sensibilidade Microbiana
Análise de Sequência de DNA
Mycobacterium tuberculosis
Susceptibility test
DNA sequencing
E test
Análise de Sequência de DNA
Testes de Sensibilidade Microbiana
title_short Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
title_full Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
title_fullStr Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
title_full_unstemmed Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
title_sort Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results
author Freixo, Maria Inês Moura
author_facet Freixo, Maria Inês Moura
Caldas, Paulo C. S.
Said, Abbadi
Martins, Fátima
Brito, Rossana Coimbra
Fonsec, Leila de Souza
Saad, Maria Helena Féres
author_role author
author2 Caldas, Paulo C. S.
Said, Abbadi
Martins, Fátima
Brito, Rossana Coimbra
Fonsec, Leila de Souza
Saad, Maria Helena Féres
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Freixo, Maria Inês Moura
Caldas, Paulo C. S.
Said, Abbadi
Martins, Fátima
Brito, Rossana Coimbra
Fonsec, Leila de Souza
Saad, Maria Helena Féres
dc.subject.other.pt_BR.fl_str_mv Mycobacterium tuberculosis
E test
Testes de Sensibilidade Microbiana
Análise de Sequência de DNA
topic Mycobacterium tuberculosis
E test
Testes de Sensibilidade Microbiana
Análise de Sequência de DNA
Mycobacterium tuberculosis
Susceptibility test
DNA sequencing
E test
Análise de Sequência de DNA
Testes de Sensibilidade Microbiana
dc.subject.en.pt_BR.fl_str_mv Mycobacterium tuberculosis
Susceptibility test
DNA sequencing
E test
dc.subject.decs.pt_BR.fl_str_mv Análise de Sequência de DNA
Testes de Sensibilidade Microbiana
description Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Rio de Janeiro, RJ, Brasil.
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2020-11-18T20:18:28Z
dc.date.available.fl_str_mv 2020-11-18T20:18:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv FREIXO, Maria Inês Moura et al. Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 107-110, Feb. 2004.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44524
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762004000100019
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv FREIXO, Maria Inês Moura et al. Antimicrobial susceptibility determined by the E test, Löwenstein-Jensen proportion, and DNA sequencing methods among Mycobacterium tuberculosis isolates discrepancies, preliminary results. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 1, p. 107-110, Feb. 2004.
0074-0276
10.1590/S0074-02762004000100019
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44524
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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