Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/9403 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
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Costa, Daniela CamargosMadureira, Ana PaulaAmaral, Lara CottaSanchez, Bruno Antônio MarinhoGomes, Luciano TeixeiraFontes, Cor Jésus FernandesLimongi, Jean EzequielBrito, Cristiana Ferreira Alves deCarvalho, Luzia Helena2015-02-02T11:15:52Z2015-02-02T11:15:52Z2014COSTA, Daniela Camargos et al. Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 109, n. 1, Feb. 2014.0074-0276https://www.arca.fiocruz.br/handle/icict/940310.1590/0074-0276140102engFundação Oswaldo CruzSubmicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de São João Del Rei. Departamento de Bioengenharia. São João Del Rey, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade Federal de Mato Grosso. Cuiabá, MT, BrasilUniversidade Federal de Mato Grosso. Cuiabá, MT, BrasilUniversidade Federal de Mato Grosso. Cuiabá, MT, BrasilCentro de Controle de Zoonoses de Uberlândia. Uberlândia, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilThe polymerase chain reaction (PCR)-based methods for the diagnosis of malaria infection are expected to accurately identify submicroscopic parasite carriers. Although a significant number of PCR protocols have been described, few studies have addressed the performance of PCR amplification in cases of field samples with submicroscopic malaria infection. Here, the reproducibility of two well-established PCR protocols (nested-PCR and real-time PCR for the Plasmodium 18 small subunit rRNA gene) were evaluated in a panel of 34 blood field samples from individuals that are potential reservoirs of malaria infection, but were negative for malaria by optical microscopy. Regardless of the PCR protocol, a large variation between the PCR replicates was observed, leading to alternating positive and negative results in 38% (13 out of 34) of the samples. These findings were quite different from those obtained from the microscopy-positive patients or the unexposed individuals; the diagnosis of these individuals could be confirmed based on the high reproducibility and specificity of the PCR-based protocols. The limitation of PCR amplification was restricted to the field samples with very low levels of parasitaemia because titrations of the DNA templates were able to detect < 3 parasites/µL in the blood. In conclusion, conventional PCR protocols require careful interpretation in cases of submicroscopic malaria infection, as inconsistent and false-negative results can occur.malariaPCRreproducibilitysubmicroscopic infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/9403/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL2014_013.pdf2014_013.pdfapplication/pdf612202https://www.arca.fiocruz.br/bitstream/icict/9403/2/2014_013.pdf0b0dfc01b7f9ecdf1fd8f04eac043720MD52TEXT2014_013.pdf.txt2014_013.pdf.txtExtracted texttext/plain40673https://www.arca.fiocruz.br/bitstream/icict/9403/3/2014_013.pdf.txt7867a3f7d45fb57ea3df767b81cd39adMD53icict/94032019-06-19 10:07:35.443oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:07:35Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| title |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| spellingShingle |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? Costa, Daniela Camargos malaria PCR reproducibility submicroscopic infection |
| title_short |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| title_full |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| title_fullStr |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| title_full_unstemmed |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| title_sort |
Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods? |
| author |
Costa, Daniela Camargos |
| author_facet |
Costa, Daniela Camargos Madureira, Ana Paula Amaral, Lara Cotta Sanchez, Bruno Antônio Marinho Gomes, Luciano Teixeira Fontes, Cor Jésus Fernandes Limongi, Jean Ezequiel Brito, Cristiana Ferreira Alves de Carvalho, Luzia Helena |
| author_role |
author |
| author2 |
Madureira, Ana Paula Amaral, Lara Cotta Sanchez, Bruno Antônio Marinho Gomes, Luciano Teixeira Fontes, Cor Jésus Fernandes Limongi, Jean Ezequiel Brito, Cristiana Ferreira Alves de Carvalho, Luzia Helena |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Costa, Daniela Camargos Madureira, Ana Paula Amaral, Lara Cotta Sanchez, Bruno Antônio Marinho Gomes, Luciano Teixeira Fontes, Cor Jésus Fernandes Limongi, Jean Ezequiel Brito, Cristiana Ferreira Alves de Carvalho, Luzia Helena |
| dc.subject.en.pt_BR.fl_str_mv |
malaria PCR reproducibility submicroscopic infection |
| topic |
malaria PCR reproducibility submicroscopic infection |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil |
| publishDate |
2014 |
| dc.date.issued.fl_str_mv |
2014 |
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2015-02-02T11:15:52Z |
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2015-02-02T11:15:52Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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COSTA, Daniela Camargos et al. Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 109, n. 1, Feb. 2014. |
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https://www.arca.fiocruz.br/handle/icict/9403 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/0074-0276140102 |
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COSTA, Daniela Camargos et al. Submicroscopic malaria parasite carriage: how reproducible are polymerase chain reaction-based methods?. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 109, n. 1, Feb. 2014. 0074-0276 10.1590/0074-0276140102 |
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Fundação Oswaldo Cruz |
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