Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil

Detalhes bibliográficos
Autor(a) principal: Dantas, Nayanne Gama Teixeira
Data de Publicação: 2015
Outros Autores: Suffys, Phillip Noel, Carvalho, Wânia da Silva, Gomes, Harrison Magdinier, Almeida, Isabela Neves, Assis, Lida Jouca de, Augusto, Claudio José, Gomgnimbou, Michel Kireopori, Refregier, Guislaine, Sola, Christophe, Miranda, Silvana Spíndola de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13393
Resumo: Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Medicina Tropical. Belo Horizonte, MG, Brasil.
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spelling Dantas, Nayanne Gama TeixeiraSuffys, Phillip NoelCarvalho, Wânia da SilvaGomes, Harrison MagdinierAlmeida, Isabela NevesAssis, Lida Jouca deAugusto, Claudio JoséGomgnimbou, Michel KireoporiRefregier, GuislaineSola, ChristopheMiranda, Silvana Spíndola de2016-03-29T17:54:47Z2016-03-29T17:54:47Z2015DANTAS, Nayanne Gama Teixeira; et al. Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil. BMC Infectious Diseases, v.15:306, 11p, Aug. 2015.1471-2334https://www.arca.fiocruz.br/handle/icict/1339310.1186/s12879-015-1057-yengBioMed CentralEpidemiologia molecularMinas GeraisMycobacterium tuberculosisResistência a medicamentosVariação genéticaMDR-TBMolecular epidemiologySpoligotypingMIRU-VNTRIS6110-RFLPMinas Gerais, BrazilGenetic diversityGenetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Medicina Tropical. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Farmácia Social. Laboratório de Biologia Molecular e Saúde Pública. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular aplicada à Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Medicina Tropical. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Farmácia Social. Laboratório de Biologia Molecular e Saúde Pública. Belo Horizonte, MG, Brasil.Fundação Ezequiel Dias. Belo Horizonte, MG, Brasil.Institut for Integrative Cell Biology. Orsay France / Centre Muraz, Bobo-Dioulasso, Burkina Faso.Institut for Integrative Cell Biology. Orsay France.Institut for Integrative Cell Biology. Orsay France.Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Medicina Tropical. Belo Horizonte, MG, Brasil.BACKGROUND: We aimed to characterize the genetic diversity of drug-resistant Mycobacterium tuberculosis (MTb) clinical isolates and investigate the molecular epidemiology of multidrug-resistant (MDR) tuberculosis from Minas Gerais State, Brazil. METHODS: One hundred and four MTb clinical isolates were assessed by IS6110-RFLP, 24-locus mycobacterial interspersed repetitive units variable-number tandem repeats (MIRU-VNTR), TB-SPRINT (simultaneous spoligotyping and rifampicin-isoniazid drug-resistance mutation analysis) and 3R-SNP-typing (analysis of single-nucleotide polymorphisms in the genes involved in replication, recombination and repair functions). RESULTS: Fifty-seven different IS6110-RFLP patterns were found, among which 50 had unique patterns and 17 were grouped into seven clusters. The discriminatory index (Hunter and Gaston, HGDI) for RFLP was 0.9937. Ninety-nine different MIRU-VNTR patterns were found, 95 of which had unique patterns and nine isolates were grouped into four clusters. The major allelic diversity index in the MIRU-VNTR loci ranged from 0.6568 to 0.7789. The global HGDI for MIRU-VNTR was 0.9991. Thirty-two different spoligotyping profiles were found: 16 unique patterns (n = 16) and 16 clustered profiles (n = 88). The HGDI for spoligotyping was 0.9009. The spoligotyped clinical isolates were phylogenetically classified into Latin-American Mediterranean (66.34 %), T (14.42 %), Haarlem (5.76 %), X (1.92 %), S (1.92 %) and U (unknown profile; 8.65 %). Among the U isolates, 77.8 % were classified further by 3R-SNP-typing as 44.5 % Haarlem and 33.3 % LAM, while the 22.2 % remaining were not classified. Among the 104 clinical isolates, 86 were identified by TB-SPRINT as MDR, 12 were resistant to rifampicin only, one was resistant to isoniazid only, three were susceptible to both drugs, and two were not successfully amplified by PCR. A total of 42, 28 and eight isolates had mutations in rpoB positions 531, 526 and 516, respectively. Correlating the cluster analysis with the patient data did not suggest recent transmission of MDR-TB. CONCLUSIONS: Although our results do not suggest strong transmission of MDR-TB in Minas Gerais (using a classical 100 % MDR-TB identical isolates cluster definition), use of a smoother cluster definition (>85 % similarity) does not allow us to fully eliminate this possibility; hence, around 20-30 % of the isolates we analyzed might be MDR-TB transmission cases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13393/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALharrison_gomes_etal_IOC_2015.pdfharrison_gomes_etal_IOC_2015.pdfapplication/pdf2492383https://www.arca.fiocruz.br/bitstream/icict/13393/2/harrison_gomes_etal_IOC_2015.pdf0a66e639e51a2a424802f463533af85eMD52TEXTharrison_gomes_etal_IOC_2015.pdf.txtharrison_gomes_etal_IOC_2015.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
title Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
spellingShingle Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
Dantas, Nayanne Gama Teixeira
Epidemiologia molecular
Minas Gerais
Mycobacterium tuberculosis
Resistência a medicamentos
Variação genética
MDR-TB
Molecular epidemiology
Spoligotyping
MIRU-VNTR
IS6110-RFLP
Minas Gerais, Brazil
Genetic diversity
title_short Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
title_full Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
title_fullStr Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
title_full_unstemmed Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
title_sort Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil
author Dantas, Nayanne Gama Teixeira
author_facet Dantas, Nayanne Gama Teixeira
Suffys, Phillip Noel
Carvalho, Wânia da Silva
Gomes, Harrison Magdinier
Almeida, Isabela Neves
Assis, Lida Jouca de
Augusto, Claudio José
Gomgnimbou, Michel Kireopori
Refregier, Guislaine
Sola, Christophe
Miranda, Silvana Spíndola de
author_role author
author2 Suffys, Phillip Noel
Carvalho, Wânia da Silva
Gomes, Harrison Magdinier
Almeida, Isabela Neves
Assis, Lida Jouca de
Augusto, Claudio José
Gomgnimbou, Michel Kireopori
Refregier, Guislaine
Sola, Christophe
Miranda, Silvana Spíndola de
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Dantas, Nayanne Gama Teixeira
Suffys, Phillip Noel
Carvalho, Wânia da Silva
Gomes, Harrison Magdinier
Almeida, Isabela Neves
Assis, Lida Jouca de
Augusto, Claudio José
Gomgnimbou, Michel Kireopori
Refregier, Guislaine
Sola, Christophe
Miranda, Silvana Spíndola de
dc.subject.other.pt_BR.fl_str_mv Epidemiologia molecular
Minas Gerais
Mycobacterium tuberculosis
Resistência a medicamentos
Variação genética
topic Epidemiologia molecular
Minas Gerais
Mycobacterium tuberculosis
Resistência a medicamentos
Variação genética
MDR-TB
Molecular epidemiology
Spoligotyping
MIRU-VNTR
IS6110-RFLP
Minas Gerais, Brazil
Genetic diversity
dc.subject.en.pt_BR.fl_str_mv MDR-TB
Molecular epidemiology
Spoligotyping
MIRU-VNTR
IS6110-RFLP
Minas Gerais, Brazil
Genetic diversity
description Universidade Federal de Minas Gerais. Faculdade de Medicina. Programa de Pós-Graduação em Doenças Infecciosas e Medicina Tropical. Belo Horizonte, MG, Brasil.
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-03-29T17:54:47Z
dc.date.available.fl_str_mv 2016-03-29T17:54:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv DANTAS, Nayanne Gama Teixeira; et al. Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil. BMC Infectious Diseases, v.15:306, 11p, Aug. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13393
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/s12879-015-1057-y
identifier_str_mv DANTAS, Nayanne Gama Teixeira; et al. Genetic diversity and molecular epidemiology of multidrug-resistant Mycobacterium tuberculosis in Minas Gerais State, Brazil. BMC Infectious Diseases, v.15:306, 11p, Aug. 2015.
1471-2334
10.1186/s12879-015-1057-y
url https://www.arca.fiocruz.br/handle/icict/13393
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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